scholarly journals Perivascular cell‐derived extracellular vesicles stimulate colorectal cancer revascularization after withdrawal of antiangiogenic drugs

2021 ◽  
Vol 10 (7) ◽  
Author(s):  
Maohua Huang ◽  
Minfeng Chen ◽  
Ming Qi ◽  
Geni Ye ◽  
Jinghua Pan ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Extracellular Vesicles ◽  
Perivascular Cell ◽  
Antiangiogenic Drugs

scholarly journals Treatment with Antiangiogenic Drugs in Multiple Lines in Patients with Metastatic Colorectal Cancer: Meta-Analysis of Randomized Trials

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
R.-D. Hofheinz ◽  
U. Ronellenfitsch ◽  
S. Kubicka ◽  
A. Falcone ◽  
I. Burkholder ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Metastatic Colorectal Cancer ◽  
Kinase Inhibitors ◽  
Meta Analysis ◽  
Progression Free Survival ◽  
Optimal Sequence ◽  
Primary Endpoints ◽  
Disease Stabilization ◽  
Antiangiogenic Drugs ◽  
The Impact

Background. In metastatic colorectal cancer (mCRC), continuing antiangiogenic drugs beyond progression might provide clinical benefit. We synthesized the available evidence in a meta-analysis.Patients and Methods. We conducted a meta-analysis of studies investigating the use of antiangiogenic drugs beyond progression. Eligible studies were randomized phase II/III trials. Primary endpoints were overall survival (OS) and progression-free survival (PFS). Secondary endpoints were the impact of continuing antiangiogenic drugs (i) in subgroups, (ii) in different types of compounds targeting the VEGF-axis (monoclonal antibodies versus tyrosine kinase inhibitors), and (iii) on remission rates and prevention of progression.Results. Eight studies (3,668 patients) were included. Continuing antiangiogenic treatment beyond progression significantly improved PFS (HR 0.64; 95%-CI, 0.55–0.75) and OS (HR 0.83; 95%-CI, 0.76–0.89). PFS was significantly improved in all subgroups with comparable HR. OS was improved in all subgroups stratified by age, gender, and ECOG status. The rate of patients achieving at least stable disease was improved with an OR of 2.25 (95%-CI, 1.41–3.58).Conclusions. This analysis shows a significant PFS and OS benefit as well as a benefit regarding disease stabilization when using antiangiogenic drugs beyond progression in mCRC. Future studies should focus on the optimal sequence of administering antiangiogenic drugs.

scholarly journals TGFBR2‑dependent alterations of microRNA profiles in extracellular vesicles and parental colorectal cancer cells

2019 ◽  
Author(s):  
Fabia Fricke ◽  
Veronika Mussack ◽  
Dominik Buschmann ◽  
Ingrid Hausser ◽  
Michael Pfaffl ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Cells ◽  
Extracellular Vesicles ◽  
Colorectal Cancer Cells

scholarly journals Hsa_circ_0004831 serves as a blood-based prognostic biomarker for colorectal cancer and its potentially circRNA-miRNA-mRNA regulatory network construction

2020 ◽  
Author(s):  
Linlin Xing ◽  
Mengyan Xia ◽  
Xin Jiao ◽  
Ling Fan
Keyword(s):  
Colorectal Cancer ◽  
Extracellular Vesicles ◽  
Regulatory Network ◽  
Prognostic Biomarker ◽  
Qpcr Assay ◽  
Gene Set Enrichment Analysis ◽  
Differentially Expressed ◽  
Expression Level ◽  
Blood Samples ◽  
Tumorigenesis And Progression

Abstract Background: Colorectal cancer (CRC) is a common malignant tumor with unsatisfactory overall prognosis. CircRNAs could be promising prognostic biomarkers in cancers, and play important role in the process of tumorigenesis and progression. Here, we explored the role of hsa_circ_0004831 in blood extracellular vesicles and its prognostic value in CRC. Methods: The circRNA and mRNA expression level matrix in extracellular vesicles of CRC and normal samples were obtained from the exoRBase database. The corresponding miRNA expression level matrix in extracellular vesicles was downloaded from the BBCancer database. Differentially expressed circRNAs, miRNAs and mRNAs were identified using the limma package of R software at the cut-off criteria of fold change (FC) > 2 and adj. p < 0.05. RT-qPCR assay was conducted to measure hsa_circ_0004831 expression level in CRC blood samples. A circRNA-miRNA-mRNA regulatory network of hsa_circ_0004831 was constructed based on competitive endogenous RNA mechanism and differentially expressed genes. The mRNAs co-expressed with hsa_circ_0004831 were screened at the cut-off criteria of pearson |r| > 0.3 and p < 0.05. Gene set enrichment analysis (GSEA) based on co-expressed mRNAs was used to explore the potential molecular function of hsa_circ_0004831. Results: Differentially expressed circRNAs, miRNAs and mRNAs were identified and hsa_circ_0004831 had a FC value of 3.92 in CRC blood extracellular vesicles. The RT-qPCR assay showed that the hsa_circ_0004831 was up-regulated in CRC blood samples. The overall survival analysis found that high expression of hsa_circ_0004831 was linked with poorer prognosis. Finally, a circRNA-miRNA-mRNA regulatory network of hsa_circ_0004831 was constructed based on down-regulated miR-4326 and 12 up-regulated mRNAs. GSEA indicated that mRNAs co-expressed with hsa_circ_0004831 were involved in EMT, WNT and p53 signaling pathways.Conclusions: The study confirmed the up-regulation of hsa_circ_0004831 in CRC, and it may act as a vital prognostic biomarker. The circRNA-miRNA-mRNA regulatory network of hsa_circ_0004831 could be used to uncover the tumorigenesis and progression of CRC.

Extracellular Vesicles in Colorectal Cancer Progression, Metastasis, Diagnosis, and Therapy

2021 ◽  
pp. 401-420
Author(s):  
Mercy Merlin ◽  
Pranav Kumar Prabhakar ◽  
Dhananjay Shukla ◽  
Atul Kumar Tiwari ◽  
Saurabh Saxena
Keyword(s):  
Colorectal Cancer ◽  
Extracellular Vesicles ◽  
Cancer Progression ◽  
Diagnosis And Therapy ◽  
Colorectal Cancer Progression

scholarly journals Analysis of cancer-related mutations in extracellular vesicles RNA by Droplet Digital™ PCR

BioTechniques ◽  
2020 ◽  
Vol 69 (2) ◽  
pp. 99-107
Author(s):  
Soo Ann Yap ◽  
Agnieszka Münster-Wandowski ◽  
Anika Nonnenmacher ◽  
Ulrich Keilholz ◽  
Sandra Liebs
Keyword(s):  
Colorectal Cancer ◽  
Tumor Progression ◽  
Extracellular Vesicles ◽  
Liquid Biopsy ◽  
Mek Inhibitor ◽  
Diagnostic Information ◽  
Braf V600e ◽  
Plasma Samples ◽  
Differential Centrifugation ◽  
Potential Biomarkers

Extracellular vesicles (EVs) are taking their place as potential biomarkers in the field of liquid biopsy. In this study, EVs were isolated from plasma samples of 31 patients with colorectal cancer and melanoma via differential centrifugation and Droplet Digital™ PCR (Bio-Rad, CA, USA) was used to profile BRAF V600E/K, KRAS G12A/C/D/V and KRAS G13D mutations from EV-derived cDNA. The concordance rates with corresponding tissue were 54% and 44% in the colorectal cancer and melanoma cohort, respectively. Two patients displayed mutations in EVs not previously detected in tissue as evidence for emerging molecular resistance to anti-EGFR and BRAF/MEK inhibitor therapy prior to radiological evidence of tumor progression. We concluded that EV-derived nucleic acids may provide clinically relevant diagnostic information and mirror evolution of the disease.

scholarly journals Plasma extracellular vesicles detected by Single Molecule array technology as a liquid biopsy for colorectal cancer

2020 ◽  
Vol 9 (1) ◽  
pp. 1809765 ◽  
Author(s):  
Ping Wei ◽  
Fei Wu ◽  
Bin Kang ◽  
Xiaohua Sun ◽  
Fabienne Heskia ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Extracellular Vesicles ◽  
Single Molecule ◽  
Liquid Biopsy ◽  
Array Technology

scholarly journals Quantitation of putative colorectal cancer biomarker candidates in serum extracellular vesicles by targeted proteomics

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Takashi Shiromizu ◽  
Hideaki Kume ◽  
Mimiko Ishida ◽  
Jun Adachi ◽  
Masayuki Kano ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Extracellular Vesicles ◽  
Cancer Biomarker ◽  
Targeted Proteomics

Proteome Analysis of Colorectal Cancer Cell Line SW480 Released Extracellular Vesicles

2018 ◽  
Vol 762 ◽  
pp. 3-7
Author(s):  
Ilva Nakurte ◽  
Kaspars Jekabsons ◽  
Elina Zandberga ◽  
Arturs Abols ◽  
Aija Line ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Extracellular Vesicles ◽  
Cancer Cell Line ◽  
Surface Protein ◽  
Proteome Analysis ◽  
Research Area ◽  
Surface Proteins ◽  
Colorectal Cancer Cell Line ◽  
Protein Fragments ◽  
Early Cancer Diagnosis

The detection and profiling of disease-specific extracellular vesicles (EVs) from body fluids has been challenging research area during recent years. However, the question – can EVs surface proteins be exploited as a credible tool for early cancer diagnosis – is still not answered. Objective of the current study was to find out whether hypoxia induces differences in protein profiles of EVs released from hypoxic human colorectal cancer cells SW480 (EVHyp) and EVs released from these cells grown in normoxic conditions – EVNorm. Obtained results show differences in EVs surface protein samples. Some protein fragments were found only or mostly in EVHyp surface protein samples. Finding of one or two such EVHyp protein fragments allows us to suggest that deciphered EVHyp surface proteins might be indices of hypoxia-induced proteome changes and might serve as a hint to find a cancer specific protein.

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