scholarly journals Comprehensive evaluation of methods for small extracellular vesicles separation from human plasma, urine and cell culture medium

2020 ◽  
Vol 10 (2) ◽  
Author(s):  
Liang Dong ◽  
Richard C. Zieren ◽  
Kengo Horie ◽  
Chi‐Ju Kim ◽  
Emily Mallick ◽  
...  
Keyword(s):  
Cell Culture ◽  
Human Plasma ◽  
Extracellular Vesicles ◽  
Culture Medium ◽  
Comprehensive Evaluation ◽  
Cell Culture Medium ◽  
Evaluation Of Methods

scholarly journals A novel three step protocol to isolate extracellular vesicles from plasma or cell culture medium with both high yield and purity

2020 ◽  
Vol 9 (1) ◽  
pp. 1791450 ◽  
Author(s):  
Xiaogang Zhang ◽  
Ellen G. F. Borg ◽  
A. Manuel Liaci ◽  
Harmjan R. Vos ◽  
Willem Stoorvogel
Keyword(s):  
Cell Culture ◽  
Extracellular Vesicles ◽  
Culture Medium ◽  
High Yield ◽  
Cell Culture Medium ◽  
Yield And Purity

Isolation of Extracellular Microvesicles from Cell Culture Medium: Comparative Evaluation of Methods

2018 ◽  
Vol 12 (2) ◽  
pp. 167-175 ◽  
Author(s):  
T. A. Shtam ◽  
R. B. Samsonov ◽  
A. V. Volnitskiy ◽  
R. A. Kamyshinsky ◽  
N. A. Verlov ◽  
...  
Keyword(s):  
Cell Culture ◽  
Comparative Evaluation ◽  
Culture Medium ◽  
Cell Culture Medium ◽  
Evaluation Of Methods

3-iodothyronamine (T1AM) is taken up and rapidly metabolized in cell culture medium only at low concentration

2020 ◽  
Author(s):  
Federica Saponaro ◽  
Marco Borsò ◽  
Sara Verlotta ◽  
Lavinia Bandini ◽  
Alessandro Saba ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture Medium ◽  
Cell Culture Medium ◽  
Low Concentration

Effect of Atmospheric-Pressure Helium Plasma Jet on Cell Culture Medium

2013 ◽  
Vol 133 (5) ◽  
pp. 278-285
Author(s):  
Norimitsu Takamura ◽  
Douyan Wang ◽  
Takao Satoh ◽  
Takao Namihira ◽  
Hisato Saitoh ◽  
...  
Keyword(s):  
Cell Culture ◽  
Atmospheric Pressure ◽  
Culture Medium ◽  
Plasma Jet ◽  
Helium Plasma ◽  
Cell Culture Medium

scholarly journals Defined MSC exosome with high yield and purity to improve regenerative activity

2021 ◽  
Vol 12 ◽  
pp. 204173142110086
Author(s):  
Jun Yong Kim ◽  
Won-Kyu Rhim ◽  
Yong-In Yoo ◽  
Da-Seul Kim ◽  
Kyoung-Won Ko ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture Medium ◽  
Density Lipoprotein ◽  
High Yield ◽  
Human Umbilical Cord ◽  
Cell Culture Medium ◽  
Human Coronary Artery ◽  
Isolation Methods ◽  
Regenerative Activity ◽  
Yield And Purity

Exosomes derived from mesenchymal stem cells (MSCs) have been studied as vital components of regenerative medicine. Typically, various isolation methods of exosomes from cell culture medium have been developed to increase the isolation yield of exosomes. Moreover, the exosome-depletion process of serum has been considered to result in clinically active and highly purified exosomes from the cell culture medium. Our aim was to compare isolation methods, ultracentrifuge (UC)-based conventional method, and tangential flow filtration (TFF) system-based method for separation with high yield, and the bioactivity of the exosome according to the purity of MSC-derived exosome was determined by the ratio of Fetal bovine serum (FBS)-derived exosome to MSC-derived exosome depending on exosome depletion processes of FBS. The TFF-based isolation yield of exosome derived from human umbilical cord MSC (UCMSC) increased two orders (92.5 times) compared to UC-based isolation method. Moreover, by optimizing the process of depleting FBS-derived exosome, the purity of UCMSC-derived exosome, evaluated using the expression level of MSC exosome surface marker (CD73), was about 15.6 times enhanced and the concentration of low-density lipoprotein-cholesterol (LDL-c), known as impurities resulting from FBS, proved to be negligibly detected. The wound healing and angiogenic effects of highly purified UCMSC-derived exosomes were improved about 23.1% and 71.4%, respectively, with human coronary artery endothelial cells (HCAEC). It suggests that the defined MSC exosome with high yield and purity could increase regenerative activity.

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