scholarly journals EGFR‐rich extracellular vesicles derived from highly metastatic nasopharyngeal carcinoma cells accelerate tumour metastasis through PI3K/AKT pathway‐suppressed ROS

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Fei Li ◽  
Xin Zhao ◽  
Rui Sun ◽  
Jinxin Ou ◽  
Junyu Huang ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Extracellular Vesicles ◽  
Carcinoma Cells ◽  
Akt Pathway ◽  
Tumour Metastasis ◽  
Metastatic Nasopharyngeal Carcinoma

scholarly journals Tumor-derived extracellular vesicles inhibit HGF/c-Met and EGF/EGFR pathways to accelerate the radiosensitivity of nasopharyngeal carcinoma cells via microRNA-142-5p delivery

2022 ◽  
Vol 8 (1) ◽  
Author(s):  
Changyu Zhu ◽  
Xiaolei Jiang ◽  
Hua Xiao ◽  
Jianmei Guan
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Extracellular Vesicles ◽  
Nude Mice ◽  
Peripheral Blood ◽  
Cell Apoptosis ◽  
Expression Patterns ◽  
Carcinoma Cells ◽  
Luciferase Reporter ◽  
Relationship Of ◽  
Dual Luciferase Reporter Assay

AbstractRadioresistance prevails as one of the largest obstacles in the clinical treatment of nasopharyngeal carcinoma (NPC). Meanwhile, tumor-derived extracellular vesicles (TEVs) possess the ability to manipulate radioresistance in NPC. However, its mechanism remains to be further explored. Therefore, the current study set out to explore the mechanism of microRNA (miR)-142-5p delivered by TEVs in regard to the radiosensitivity of NPC. Firstly, peripheral blood samples were collected from patients with radioresistance and radiosensitivity, followed by RT-qPCR detection of miR-142-5p expression. A dual-luciferase reporter assay was carried out to elucidate the targeting relationship of miR-142-5p with HGF and EGF. In addition, radiotherapy-resistant NPC cell models were established by screening NPC cells with gradient increasing radiation exposure, and co-incubated with EVs isolated from miR-142-5p mimic-transfected NPC cells, followed by overexpression of HGF and EGF. Moreover, cell viability was detected by means of MTS, cell proliferation with a colony formation assay, cell apoptosis with flow cytometry, and expression patterns of related genes with the help of Western blot analysis. NPC xenotransplantation models in nude mice were also established by subcutaneous injection of 5-8FR cells to determine apoptosis, tumorigenicity, and radiosensitivity in nude mice. It was found that miR-142-5p was poorly expressed in peripheral blood from NPC patients with radioresistance. Mechanistic experimentation illustrated that miR-142-5p inversely targeted HGF and EGF to inactivate the HGF/c-Met and EGF/EGFR pathways, respectively. NPC cell apoptosis was observed to be augmented, while their radioresistance and proliferation were restricted by EVs-miR-142-5p or HGF silencing, or EGF silencing. Furthermore, EVs-miR-142-5p inhibited growth and radioresistance and accelerated the apoptosis of radiotherapy-resistant NPC cells in nude mice by inhibiting the HGF/c-Met and EGF/EGFR pathways. Collectively, our findings indicated that TEVs might inhibit the HGF/c-Met and EGF/EGFR pathways by delivering miR-142-5p into radiotherapy-resistant NPC cells to enhance radiosensitivity in NPC.

scholarly journals Anti-apoptotic role of TWIST and its association with Akt pathway in mediating taxol resistance in nasopharyngeal carcinoma cells

2007 ◽  
Vol 120 (9) ◽  
pp. 1891-1898 ◽  
Author(s):  
Xiaomeng Zhang ◽  
Qi Wang ◽  
Ming-Tat Ling ◽  
Yong Chuan Wong ◽  
Steve C.L. Leung ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Carcinoma Cells ◽  
Akt Pathway ◽  
Taxol Resistance

Differentiation of highly metastatic nasopharyngeal carcinoma cells using multiphoton microscopy

2016 ◽  
Author(s):  
Zhenlin Zhan ◽  
Zhenzhen Sun ◽  
Jingwen Li ◽  
Qing Ye ◽  
Shuangmu Zhuo ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Multiphoton Microscopy ◽  
Carcinoma Cells ◽  
Metastatic Nasopharyngeal Carcinoma

scholarly journals LMP1‐positive extracellular vesicles promote radioresistance in nasopharyngeal carcinoma cells through P38 MAPK signaling

2019 ◽  
Vol 8 (13) ◽  
pp. 6082-6094 ◽  
Author(s):  
Zhibao Zhang ◽  
Xuehui Yu ◽  
Zhuan Zhou ◽  
Bo Li ◽  
Jinwu Peng ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
P38 Mapk ◽  
Extracellular Vesicles ◽  
Mapk Signaling ◽  
Carcinoma Cells
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