Use of scanning electron microscopy to analyze sculpturing pattern and internal features of pollen grain wall in some members of Astragaleae (subfamily: Papilionoidae)

2021 ◽  
Author(s):  
Neelam Rashid ◽  
Muhammad Zafar ◽  
Mushtaq Ahmad ◽  
Khafsa Malik ◽  
Syed Nasar Shah ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Grain ◽  
Pollen Grain Wall ◽  
Scanning Electron

scholarly journals The ultrastructure of pollen grain surface in allotetraploid petunia (Petunia hybrida hort. superbissima) as revealed by scanning electron microscopy

2015 ◽  
Vol 46 (4) ◽  
pp. 599-601
Author(s):  
S. Muszyński ◽  
J. Kocoń ◽  
W. Guzowski ◽  
M. Bieguński
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Petunia Hybrida ◽  
Pollen Grain ◽  
Characteristic Pattern ◽  
Grain Surface ◽  
Pollen Grain Wall ◽  
Scanning Electron

The ultrastructure of pollen grain surface in allotetraploid petunias was analyzed by scanning electron microscopy. The pollen grain wall is developed into characteristic pattern of convulations.

scholarly journals Pollen grain surface in Vaccinium myrtillus as seen in scanning electron microscopy

2014 ◽  
Vol 50 (4) ◽  
pp. 583-584
Author(s):  
Józef Kocoń ◽  
Kazimierz Pliszka ◽  
Stanisław Muszyński
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Grains ◽  
Pollen Grain ◽  
Vaccinium Myrtillus ◽  
Grain Surface ◽  
Scanning Electron

Pollen grain surface of <em>Vaccinium myrtillus</em> L. was analyzed by scanning electron microscopy. Pollen grains remain in tetrahedral tetrads. Grain surface is verrucose, consisting of thick, irregularly shaped muri, surrounding small, round or oval lumina. The surface of the muri is fissured, and minute papillae can also be noted.

Stipa ×fallax (Poaceae: Pooideae: Stipeae), a new natural hybrid from Tajikistan, and a new combination in Stipa drobovii

Phytotaxa ◽  
2017 ◽  
Vol 303 (2) ◽  
pp. 141 ◽  
Author(s):  
MARCIN NOBIS ◽  
ARKADIUSZ NOWAK ◽  
AGNIESZKA NOBIS ◽  
SYLWIA NOWAK ◽  
JUSTYNA ŻABICKA ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Central Asia ◽  
Field Observation ◽  
Parental Species ◽  
Pollen Grain ◽  
Numerical Analyses ◽  
New Combination ◽  
Natural Hybrid ◽  
Scanning Electron

Stipa ×fallax nothosp. nov. (Poaceae), from western Pamir Alai Mts (Tajikistan), is described and illustrated. Field observation, numerical analyses of morphology, and pollen grain viability data show that it originated from hybridization between S. drobovii and S. macroglossa subsp. macroglossa, species representing sections Smirnovia and Stipa, respectively. Stipa ×fallax is morphologically close to S. ×alaica and S. ×hissarica, but is distinguished by its shortly pilose lower part of the awn and densely pubescent leaves. Characters distinguishing S. ×fallax from its parental species as well as similar hybrid taxa in section Smirnovia that also grow in Central Asia are presented. The micromorphology of lemmas and leaves of S. ×fallax and its parental species was examined by scanning electron microscopy. We also propose the new combination Stipa drobovii var. iskanderkulica (Tzvelev) M.Nobis & A.Nowak.

scholarly journals Pollen morphology of Prototulbaghia Vosa: A comparative palynological study of the Southern African Alliaceae.

2013 ◽  
Vol 32 (1) ◽  
Author(s):  
Melissa Andriessen ◽  
Madeleen Struwig ◽  
Stefan J. Siebert
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Morphology ◽  
Pollen Grain ◽  
Evolutionary Significance ◽  
Monotypic Genus ◽  
Taxonomic Characteristic ◽  
Unknown Event ◽  
Palynological Study ◽  
Scanning Electron

The  Southern  African  Alliaceae  Borkh.  is  represented  by  four genera (Allium L., Nothoscordum Kunth, Tulbaghia L. and Prototulbaghia Vosa) and 28 species. The  pollen  morphology  of  the  endangered  monotypic  genus Prototulbaghia has  not  been described  before.  A  comparative  study  of  the  pollen  morphology  of Prototulbaghia  siebertii Vosa, Nothoscordum  borbonicum Kunth, Tulbaghia  simmleri P.Beauv.  and T.  violaceae Harv.  is presented in this article. Scanning electron microscopy, as well as light microscopy, were used to examine the pollen. The pollen morphology of the species can be described as perprolate and monosulcate, and the surface sculpture as reticulate and heterobrochate. However, the pollen of Prototulbaghia siebertii displays a unique characteristic as the grains are folded in their breadth with the tips touching, hence causing the grain to display a triangular and disulcate appearance. It might be possible to ascribe this fold to the process of harmomegathy or a still unknown event that occurs during the development of the pollen grain. This phenomenon should be further investigated to determine the cause of folding and whether it is a unique taxonomic  characteristic  of  this  genus,  and  if  it  could  be  of  evolutionary  significance  for the Alliaceae.

Pollen Presenter and Pollen Morphology of Banksia L.f. (Proteaceae)

1993 ◽  
Vol 41 (5) ◽  
pp. 439 ◽  
Author(s):  
M Sedgley ◽  
M Sierp ◽  
MA Wallwork ◽  
AM Fuss ◽  
K Thiele
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Morphology ◽  
Pollen Grain ◽  
Cylindrical Structures ◽  
Scanning Electron

The morphology of the pollen presenter and pollen was studied in 79 taxa of Banksia using scanning electron microscopy. There were three major types of pollen presenter. Elongated and cylindrical structures were characteristic of the series Banksia, Crocinae, Tetragonae, Coccineae and some species of the Cyrtostylis and Abietinae. Short and cylindrical pollen presenters were found in the series Salicinae, Quercinae, Prostratae and some species of the Cyrtostylis. Short and ovoid pollen presenters were found in the series Grandes and Dryandroideae, and in the species B. brownii and B. ericifolia of the Spicigerae and B. pulchella of the Abietinae. The pollen-receptive stigma cells of all taxa were enclosed within a groove, which in the majority of species was obliquely terminal and longitudinal. Subterminal grooves, all of which were longitudinal, except that of B. speciosa which was located laterally, were observed in a few species. Pollen of all taxa had two germination pores, at opposite ends of a cylindrical, ovoid or crescent-shaped grain. There was a higher level of between species and between group consistency in pollen grain than in pollen presenter structure. The results have implications for both the interspecific fertility relationships and taxonomy of the genus.

scholarly journals Pollen Morphology of Pinus mugo Turra × Pinus sylvestris L. Hybrids and Parental Species in an Experimental Culture

2015 ◽  
Vol 57 (1) ◽  
pp. 149-160 ◽  
Author(s):  
Joanna Bykowska ◽  
Małgorzata Klimko
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Morphology ◽  
Parental Species ◽  
Pollen Grains ◽  
Grain Morphology ◽  
Pollen Grain ◽  
Pinus Mugo ◽  
Pollen Grain Morphology ◽  
Scanning Electron

Abstract Pollen grains of Pinus mugo, P. sylvestris and P. ×rhaetica (= P. mugo × P. sylvestris) were examined by light and scanning electron microscopy. The pollen grains were bisaccate and monosulcate. The corpus-saccus attachment was distinct. The pollen corpus exine sculpture was verrucate-rugulate and deeply sculptured. The surface of the tectum was covered with small grana and it was perforate. The saccus sexine ornamentation was reticulate and irregularly perforate. The tectum surface characters in the proximal and distal view of the corpus and saccus were less variable and they did not provide good criteria to identify the species under study. However, some differences were observed in the size of elevation on the corpus between pollen grains of the same species and between the parental species. This study of the pollen grain morphology of the corpus and saccus provided some important new data.

Pollen Morphology of the Genus Tragopogon (Asteraceae)

2021 ◽  
Vol 63 (1-2) ◽  
pp. 31-43
Author(s):  
H. Azizi ◽  
M. Sheidai ◽  
V. Mozaffarian ◽  
Z. Noormohammadi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Morphology ◽  
Polar View ◽  
Pollen Grain ◽  
The Other ◽  
Prolate Spheroidal ◽  
Old World ◽  
Oblate Spheroidal ◽  
Scanning Electron

Tragopogon L. (Cichorioideae, Lactuceae, Scorzonerinae) is an Old World genus with 150 species. Pollen morphology has proved useful in the systematics of some genera and species of Asteraceae as well as in that of some of its genera and species. The pollen morphology of 24 taxa of the genus Tragopogon was investigated in detail by scanning electron microscopy (SEM). The pollen grain type ranged from suboblate, oblate-spheroidal to prolate-spheroidal in equatorial view and hexagonal, obtuse-hexagonal to hexagonal-angular in polar view. In this study separation of the species of the sections Majores, Profundisulcati, Sosnovsky, Chromopappus, Rubriflori according to Flora Iranica is presented from the other species of Tragopogon. T. jezdianus, T. porphyrocephalus, T. rezaiyensis are suggested to belong to Rubriflori section. The results indicate that the palynological characters of the genus Tragopogon are valuable for taxonomic applications and are useful for classification.

Leaf Micromorphology and Pollen Grain Characteristics of Nicotiana glauca. R.C. Graham and Nicotiana tabacum. L.(Solanaceae, Nicotianoideae)

2021 ◽  
pp. 25-33
Author(s):  
Farag M. El-Mokasabi ◽  
Abdelbaset M. Asker ◽  
Raja M. El-Mabrouk
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Nicotiana Tabacum ◽  
Light Microscope ◽  
Pollen Grain ◽  
Nicotiana Glauca ◽  
Leaf Venation ◽  
Leaf Micromorphology ◽  
Nicotiana Tabacum L ◽  
Scanning Electron

The micromorphological variation of the leaf and pollen grain characteristics in two species of Nicotiana was examined using a light microscope (LM) and scanning electron microscopy (SEM). These species are Nicotiana glauca R.C. Graham (wild species) and Nicotiana tabacum L. (cultivated species). The leaves are simple. The other leaf micromorphological features presented variation. The leaf architectural characteristics were also observed. Leaf venation is pinnate brochidodromous. The epidermal cell shape, on the abaxial leaf surface, is polygonal to irregular in Nicotiana glauca but it is so adaxially in all the two species the cells are irregular on both the epidermal layers. The anticlinal walls are either straight or sinuous. The leaves are amphistomatic. The stomata are commonly anomocytic to anisocytic. Foliar trichomes are glandular and non-glandular. Some of the foliar micromorphological characters as viewed under SEM were beneficial in species identification. The pollen shape is prolate with aperture tricolpate without opercula, the colpi shallow sunken with their ends acute. The exine sculpture is reticulate with granulate or granulate-microperforated to fine granulate and irregularly reticulate to perforate. Keywords: Leaf micromorphology- Pollen grains - Light microscope (LM); Scanning electron microscopy (SEM)- Nicotiana Solanaceae -Nicotianoideae.

Fine Structure of Pollen Grain Ektexine of Maize, Teosinte and Tripsacum

1972 ◽  
Vol 30 ◽  
pp. 226-227
Author(s):  
Umesh C. Banerjee ◽  
Elso S. Barghoorn
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Zea Mays ◽  
Fine Structure ◽  
Light Microscope ◽  
Pollen Grains ◽  
Pollen Grain ◽  
Wild Relatives ◽  
Distilled Water ◽  
Scanning Electron

Using light microscope (LM) it was found very difficult to distinguish the ektexine (outer sculptured layer of exine) pattern of maize (Zea mays L.) pollen grains from that of its wild relatives teosinte (Euchlaena mexicana Schrad.) and tripsacum (Tripsacum spp.). At the magnifications obtained by LM, the pollen grain ektexine is faintly granular or netted. By the use of electron microscopy, however, it is possible to characterize their pollen ektexine patterns.For scanning electron microscopy (SEM) acetolysed pollen grains were used. After acetolysis the pollen samples were washed several times in glass-distilled water to remove traces of acids. Each sample was dispersed in a drop of distilled water placed on specimen holders.

Pollen grain micromorphology in some genera of Ornithogaleae (Asparagaceae, Scilloideae) and its relative significance

Phytotaxa ◽  
2020 ◽  
Vol 458 (4) ◽  
pp. 242-256
Author(s):  
MEHDI HEIDARIAN ◽  
SEYED MOHAMMAD MAHDI HAMDI ◽  
MOHAMMAD MEHDI DEHSHIRI ◽  
TAHER NEJADSATTARI ◽  
SEYED MOHAMMAD MASOUMI
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Grains ◽  
Pollen Grain ◽  
Relative Significance ◽  
Phylogenetic Studies ◽  
Transmission Electron ◽  
Exine Ornamentation ◽  
Tem Transmission Electron Microscopy ◽  
Scanning Electron

In this research, the pollen micromorphology of nine species belonging to Honorius, Loncomelos, Ornithogalum and Stellarioides are observed by LM (Light Microscopy), SEM (Scanning Electron Microscopy) and two species by TEM (Transmission Electron Microscopy). Lately, the phylogenetic studies have determined nineteen monophyletic genera in subfamily Ornithogaloideae (=tribe Ornithogaleae). Our results show that the pollen grains characters such as shape, size, exine ornamentation on equatorial surface, sulcus margin exine ornamentation, sulcus membrane ornamentation and lumina number on exine surface are varied among all studied genera. The dendrogram of palynological characters analyzed on LM and SEM observation by MVSP (Multi Variate Statistical Package) software partly supports the monophyletic genera. According to the obtained palynological dendrogram, Loncomelos arcuatum and Ornithogalum sintenisii are placed in one subtype. Based on the sporoderm structure (exine thickness and the endexine type), L. arcuatum differs from O. sintenisii. Finally, the palynological characters can be useful for clarifying the taxonomic relationship in this subfamily.

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