Design of two natural deproteinized bovine bone scaffolds and evaluation of the effect of initial cell seeding density on repairing bone defects

2021 ◽  
Author(s):  
Peng Chen ◽  
Jie Xie ◽  
Jianxi Zhu ◽  
Yihe Hu
Keyword(s):  
Bone Defects ◽  
Seeding Density ◽  
Bovine Bone ◽  
Cell Seeding ◽  
Initial Cell ◽  
Bone Scaffolds ◽  
Cell Seeding Density ◽  
Deproteinized Bovine Bone

Effect of Initial Cell-Seeding Density on Postoperative Cell Number and Dispersion

2005 ◽  
Vol 11 (11-12) ◽  
pp. 1898-1904 ◽  
Author(s):  
Jason Shearn ◽  
Lauren Hellmann ◽  
Gregory Boivin
Keyword(s):  
Cell Number ◽  
Seeding Density ◽  
Cell Seeding ◽  
Initial Cell ◽  
Cell Seeding Density

scholarly journals Effect of initial cell seeding density on 3D-engineered silk fibroin scaffolds for articular cartilage tissue engineering

Biomaterials ◽  
2011 ◽  
Vol 32 (34) ◽  
pp. 8927-8937 ◽  
Author(s):  
Sarmistha Talukdar ◽  
Quynhhoa T. Nguyen ◽  
Albert C. Chen ◽  
Robert L. Sah ◽  
Subhas C. Kundu
Keyword(s):  
Tissue Engineering ◽  
Articular Cartilage ◽  
Silk Fibroin ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Seeding Density ◽  
Cell Seeding ◽  
Initial Cell ◽  
Cell Seeding Density

Size Regulation of Chondrocyte Spheroids Using a PDMS-Based Cell Culture Chip

2013 ◽  
Vol 25 (4) ◽  
pp. 644-649 ◽  
Author(s):  
Takahisa Anada ◽  
◽  
Osamu Suzuki
Keyword(s):  
Tissue Engineering ◽  
Cell Differentiation ◽  
Chondrogenic Differentiation ◽  
Seeding Density ◽  
Cell Seeding ◽  
Initial Cell ◽  
Cell Seeding Density ◽  
Tissue Engineering Approach ◽  
Cellular Aggregates

Cartilage self-repair is limited due to a lack of blood supply and the low mitosis rate of chondrocytes. A tissue engineering approach using cells and biomaterials has the potential to treat cartilage injury. Threedimensional cellular aggregates are an excellent model for mimicking condensation and chondrogenic differentiation in vitro. We developed a technique for constructing spheroids utilizing a polydimethylsiloxane (PDMS)-based culture chip. The objective of this study is to determine how the initial cell density on a culture chip affects the chondrogenic ATDC5 cell differentiation. We demonstrate how culture chips having arrays of multicavities are able to generate high numbers of uniform spheroids rapidly and simultaneously with narrow size distribution. Spheroids are collected easily and noninvasively. Higher cell seeding density on the culture chip enhances chondrogenic cell differentiation. These results suggest the usefulness of this chip in engineering 3D cellular constructs with high functionality for tissue engineering.

scholarly journals Injectable, redox-polymerized carboxymethylcellulose hydrogels promote nucleus pulposus-like extracellular matrix elaboration by human MSCs in a cell density-dependent manner

2018 ◽  
Vol 33 (4) ◽  
pp. 576-589 ◽  
Author(s):  
Devika M Varma ◽  
Michelle S DiNicolas ◽  
Steven B Nicoll
Keyword(s):  
Extracellular Matrix ◽  
Minimally Invasive ◽  
Nucleus Pulposus ◽  
Seeding Density ◽  
Human Mscs ◽  
Dependent Manner ◽  
Cell Seeding ◽  
Swelling Properties ◽  
Matrix Deposition ◽  
Cell Seeding Density

Low back pain is a major cause for disability and is closely linked to intervertebral disc degeneration. Mechanical and biological dysfunction of the nucleus pulposus in the disc has been found to initiate intradiscal degenerative processes. Replacing or enriching the diseased nucleus pulposus with an injectable, stem cell-laden biomaterial that mimics its material properties can provide a minimally invasive strategy for biological and structural repair of the tissue. In this study, injectable, in situ-gelling carboxymethylcellulose hydrogels were developed for nucleus pulposus tissue engineering using encapsulated human marrow-derived mesenchymal stromal cells (hMSCs). With the goal of obtaining robust extracellular matrix deposition and faster construct maturation, two cell-seeding densities, 20 × 106 cells/ml and 40 × 106 cells/ml, were examined. The constructs were fabricated using a redox initiation system to yield covalently crosslinked, cell-seeded hydrogels via radical polymerization. Chondrogenic culture of the hydrogels over 35 days exhibited high cell viability along with deposition of proteoglycan and collagen-rich extracellular matrix, and mechanical and swelling properties similar to native human nucleus pulposus. Further, the matrix production and distribution in the carboxymethylcellulose hydrogels was found to be strongly influenced by hMSC-seeding density, with the lower cell-seeding density yielding a more favorable nucleus pulposus-specific matrix phenotype, while the rate of construct maturation was less dependent on the cell-seeding density. These findings are the first to demonstrate the utility of redox-polymerized carboxymethylcellulose hydrogels as hMSC carriers for potential minimally invasive treatment strategies for nucleus pulposus replacement.

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