Ultrastructural and immunohistochemical study of phenotypic switch in gastrointestinal smooth muscle cells

2018 ◽  
Vol 81 (11) ◽  
pp. 1233-1240 ◽  
Author(s):  
María José Luesma ◽  
Irene Cantarero ◽  
Tomás Castiella ◽  
Ana Isabel Sánchez‐Cano ◽  
Pablo Iruzubieta ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Immunohistochemical Study ◽  
Muscle Cells ◽  
Phenotypic Switch ◽  
Gastrointestinal Smooth Muscle

scholarly journals MiR-9 promotes the phenotypic switch of vascular smooth muscle cells by targeting KLF5

2019 ◽  
Author(s):  
XIAOCHUN LU ◽  
SHITANG MA ◽  
BO ZHOU ◽  
TIELING LI
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
High Glucose ◽  
Muscle Cells ◽  
Phenotypic Switch ◽  
Vsmc Proliferation ◽  
Qrt Pcr ◽  
And Migration ◽  
Proliferation And Migration

Background: Diabetic vascular smooth muscle cells (VSMCs) are characterized by increased proliferation and migration. Small non-coding microRNAs (miRNAs) have been considered critical modulators of VSMC phenotypic switch after an environmental stimulus. However, microRNA in high glucose-induced pro-inflammation and its atherogenic effect is still ambiguous. Methods: qRT-PCR was used to examine the expression of miR-9 in VSMCs. The downstream signaling protein relative to miR-9 regulation, Krüppel-like factor 5, and some marker genes of contractile VSMCs, were analyzed by western blotting and qRT-PCR. Luciferase reporter assay was used to detect the expression of KLF5, which is regulated by miR-9. To examine the function of a miR-9 inhibitor in VSMC proliferation and migration, VSMC proliferation and migration assays were performed. Results: Reduced transcriptional levels of miR-9 and expression of specific genes of contractile VSMCs were observed in the SMC cell line C-12511 treated with high glucose and SMCs, which were isolated from db/db mice. Moreover, the activity of KLF5 3′-UTR was dramatically reduced by a miR-9 mimic, and increased by a miR-9 inhibitor. The proliferation and migration of SMCs was reduced by the miR-9 mimic. Conclusion: miR-9 inhibits the proliferation and migration of SMC by targeting KLF5 in db/db mice. Keywords: miR-9; Smooth muscle cells; Proliferation; Migration; KLF5

scholarly journals Depolarization-Stimulated Contractility of Gastrointestinal Smooth Muscle in Calcium-Free Solution: A Review

2011 ◽  
Vol 2011 ◽  
pp. 1-3 ◽  
Author(s):  
Emily D. Evans ◽  
Allen W. Mangel
Keyword(s):  
Smooth Muscle ◽  
Membrane Potential ◽  
Smooth Muscle Cells ◽  
Smooth Muscles ◽  
Muscle Cells ◽  
Threshold Level ◽  
Slow Waves ◽  
Free Solution ◽  
Gastrointestinal Smooth Muscle ◽  
Small Muscle

The membrane of most gastrointestinal smooth muscles shows slow waves, slow rhythmic changes in membrane potential. Slow waves serve to bring the membrane potential of smooth muscle cells to a threshold level that elicits a second electrical event known as the spike or action potential. The inward current of the spike, in most gastrointestinal smooth muscle preparations, is carried, at least in part, by calcium. Indeed, considering the narrow diameter of smooth muscle cells, some have hypothesized that the influx of calcium during the spike is sufficient for activation of the contractile machinery. Findings consistent with this include marked reduction in contractility during exposure of muscle segments to blockers of L-type calcium channels or following reductions in external calcium levels. However, it has also been observed that following exposure of muscle segments to external bathing solutions containing no added calcium plus 5 mM EGTA to remove any remaining extracellular calcium, contractions can be triggered following membrane depolarization. It is noteworthy that in isolated smooth muscle cells or in small muscle segments, during incubation in calcium-free solution, depolarization does not induce contractions. The present paper discusses the evidence in support of depolarization-mediated contractions occurring in gastrointestinal smooth muscle segments during incubation in solutions devoid of calcium.

scholarly journals The Role of Smooth Myocytes and Macrophages in Development of Complicated Forms of Arterial Atherosclerosis

Kardiologiia ◽  
2019 ◽  
Vol 59 (1) ◽  
pp. 57-61
Author(s):  
S. S. Todorov
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Immunohistochemical Study ◽  
Muscle Cells ◽  
External Carotid ◽  
Atherosclerotic Plaques ◽  
Unstable Atherosclerotic Plaque ◽  
Immunohistochemical Studies ◽  
Smooth Myocytes

Purpose:to conduct morphohistochemical and immunohistochemical study of arterial unstable atherosclerotic plaques for assessment of the state of smooth muscle cells (SMC) and macrophages.Materials and methods.The surgical material of the peripheral arteries (femoral, popliteal, external carotid) was obtained from 50 patients aged over 60 years, followed by morphohistochemical, immunohistochemical studies.Results.Hyperplasia of secretory smooth muscle cells (SMC), and new formation of thin-walled capillary vessels was noted in unstable atherosclerotic plaques. Macrophagic infiltration was detected in the intima of arteries, in places of accumulation of foam cells.Conclusion.Unstable atherosclerotic plaque is a cellular-intercellular process with the participation of lipids, macrophages, and with predominance of SMC and newly formed vessels.

Identification of distinct molecular phenotypes in cultured gastrointestinal smooth muscle cells

1998 ◽  
Vol 115 (3) ◽  
pp. 605-617 ◽  
Author(s):  
Jacqueline Brittingham ◽  
Christopher Phiel ◽  
Wendy C. Trzyna ◽  
Vijayalakshmi Gabbeta ◽  
Kirk M. McHugh
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Gastrointestinal Smooth Muscle ◽  
Molecular Phenotypes

The epigenetic phenotypic switch of vascular smooth muscle cells involved in atherosclerosis

The Lancet ◽  
2013 ◽  
Vol 381 ◽  
pp. S34 ◽  
Author(s):  
Mark Davies ◽  
Jennifer Harman ◽  
Haixiang Yu ◽  
Martin Bennett ◽  
Helle Jørgensen
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
Phenotypic Switch

The effects of Weichangshu tablet on intracellular Ca2+ concentration in cultured rat gastrointestinal smooth muscle cells

2011 ◽  
Vol 67 (2) ◽  
pp. 265-273
Author(s):  
Shuai Zhou ◽  
Luping Zhang ◽  
Dongmei Zhao ◽  
Guoying Feng ◽  
Xiaodong Song ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Gastrointestinal Smooth Muscle
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