High-speed quantitative 3D imaging by dual-illumination holographic microscopy

2018 ◽  
Vol 81 (12) ◽  
pp. 1361-1365
Author(s):  
Dario Donnarumma ◽  
Nitin Rawat ◽  
Alexey Brodoline
Keyword(s):  
High Speed ◽  
3D Imaging ◽  
Holographic Microscopy

scholarly journals The human sperm beats anisotropically and asymmetrically in 3D

2019 ◽  
Author(s):  
Hermes Gadêlha ◽  
Paul Hernández-Herrera ◽  
Fernando Montoya ◽  
Alberto Darszon ◽  
Gabriel Corkidi
Keyword(s):  
Ion Channels ◽  
Mathematical Analysis ◽  
Molecular Motors ◽  
High Speed ◽  
3D Imaging ◽  
Human Sperm ◽  
Travelling Wave ◽  
Fundamental Question ◽  
Sperm Flagellum ◽  
Straight Line

The canonical beating of the human sperm flagellum is postulated to be symmetric. This is despite the reported asymmetries inherent to the flagellar axonemal structure, from distribution and activation of molecular motors to, even, the localisation of regulatory ion channels. This raises a fundamental question: how symmetric beating is possible within such intrinsically asymmetric flagellar complex? Here, we employ high-speed 3D imaging with mathematical analysis capable of resolving the flagellar movement in 4D (3D+time). This reveals that the human sperm beating is both anisotropic and asymmetric, and composed by a superposition of two transversal waves: an asymmetric travelling wave and a symmetric standing wave. This novel anisotropic travelling-pulsation mechanism induces sperm rolling self-organisation and causes a flagellar kinematic illusion, so that the beat appears to be symmetric if observed with 2D microscopy. The 3D beating anisotropy thus regularises the intrinsic flagellar asymmetry to achieve symmetric side-to-side movement and straight-line swimming.

Fast 3D imaging algorithm for high-speed spinning targets based on GRT-CLEAN

2018 ◽  
Vol 33 (3) ◽  
pp. 228-238
Author(s):  
王 会 WANG Hui ◽  
巨 欢 JU Huan ◽  
方 阳 FANG Yang ◽  
李荣旭 LI Rong-xu ◽  
王保平 WANG Bao-ping
Keyword(s):  
High Speed ◽  
3D Imaging ◽  
Imaging Algorithm ◽  
Spinning Targets

High-speed 3D imaging of the beating heart using temporal estimation

1987 ◽  
Vol 39 (3) ◽  
pp. 279-290 ◽  
Author(s):  
R.S. Acharya ◽  
P.B. Heffernan ◽  
R.A. Robb ◽  
H. Wechsler
Keyword(s):  
High Speed ◽  
3D Imaging ◽  
Beating Heart ◽  
Temporal Estimation

scholarly journals High-speed Imaging at 3 Tesla: A Technical and Clinical Review with an Emphasis on Whole-brain 3D Imaging

2004 ◽  
Vol 3 (4) ◽  
pp. 177-187 ◽  
Author(s):  
Shinji NAGANAWA ◽  
Hisashi KAWAI ◽  
Hiroshi FUKATSU ◽  
Takeo ISHIGAKI ◽  
Tomomi KOMADA ◽  
...  
Keyword(s):  
Clinical Review ◽  
High Speed ◽  
3D Imaging ◽  
3 Tesla ◽  
High Speed Imaging ◽  
Whole Brain
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