scholarly journals A beginner's guide to atomic force microscopy probing for cell mechanics

2016 ◽  
Vol 80 (1) ◽  
pp. 75-84 ◽  
Author(s):  
Núria Gavara
Keyword(s):  
Atomic Force Microscopy ◽  
Cell Mechanics ◽  
Force Microscopy ◽  
Atomic Force

Quantitative mechanical analysis of indentations on layered, soft elastic materials

Soft Matter ◽  
2019 ◽  
Vol 15 (8) ◽  
pp. 1776-1784 ◽  
Author(s):  
Bryant L. Doss ◽  
Kiarash Rahmani Eliato ◽  
Keng-hui Lin ◽  
Robert Ros
Keyword(s):  
Atomic Force Microscopy ◽  
Elastic Modulus ◽  
Cell Mechanics ◽  
Biological Samples ◽  
Mechanical Analysis ◽  
Elastic Materials ◽  
Mechanical Heterogeneity ◽  
Popular Method ◽  
Force Microscopy ◽  
Atomic Force

Atomic force microscopy (AFM) is becoming an increasingly popular method for studying cell mechanics, however the existing analysis tools for determining the elastic modulus from indentation experiments are unable to quantitatively account for mechanical heterogeneity commonly found in biological samples.

scholarly journals Investigating cell mechanics with atomic force microscopy

2015 ◽  
Vol 12 (104) ◽  
pp. 20140970 ◽  
Author(s):  
Kristina Haase ◽  
Andrew E. Pelling
Keyword(s):  
Atomic Force Microscopy ◽  
Cell Mechanics ◽  
Normal Cell ◽  
Mechanical Characterization ◽  
Nonlinear Models ◽  
Single Cells ◽  
Mechanistic Models ◽  
Force Microscopy ◽  
Atomic Force ◽  
Inelastic Responses

Transmission of mechanical force is crucial for normal cell development and functioning. However, the process of mechanotransduction cannot be studied in isolation from cell mechanics. Thus, in order to understand how cells ‘feel’, we must first understand how they deform and recover from physical perturbations. Owing to its versatility, atomic force microscopy (AFM) has become a popular tool to study intrinsic cellular mechanical properties. Used to directly manipulate and examine whole and subcellular reactions, AFM allows for top-down and reconstitutive approaches to mechanical characterization. These studies show that the responses of cells and their components are complex, and largely depend on the magnitude and time scale of loading. In this review, we generally describe the mechanotransductive process through discussion of well-known mechanosensors. We then focus on discussion of recent examples where AFM is used to specifically probe the elastic and inelastic responses of single cells undergoing deformation. We present a brief overview of classical and current models often used to characterize observed cellular phenomena in response to force. Both simple mechanistic models and complex nonlinear models have been used to describe the observed cellular behaviours, however a unifying description of cell mechanics has not yet been resolved.

scholarly journals Investigation into local cell mechanics by atomic force microscopy mapping and optical tweezer vertical indentation

2015 ◽  
Vol 27 (6) ◽  
pp. 065102 ◽  
Author(s):  
G Coceano ◽  
M S Yousafzai ◽  
W Ma ◽  
F Ndoye ◽  
L Venturelli ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Cell Mechanics ◽  
Optical Tweezer ◽  
Force Microscopy ◽  
Atomic Force ◽  
Local Cell

High-throughput measurements of cell mechanics using atomic force microscopy with micro-patterned substrates

MHS2013 ◽  
2013 ◽  
Author(s):  
Ryosuke Takahashi ◽  
Kaori Kuribayashi-Shigetomi ◽  
Masahiro Tsuchiya ◽  
Agus Subagyo ◽  
Kazuhisa Sueoka ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
High Throughput ◽  
Cell Mechanics ◽  
Patterned Substrates ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals The temperature dependence of cell mechanics measured by atomic force microscopy

2009 ◽  
Vol 6 (2) ◽  
pp. 025009 ◽  
Author(s):  
R Sunyer ◽  
X Trepat ◽  
J J Fredberg ◽  
R Farré ◽  
D Navajas
Keyword(s):  
Atomic Force Microscopy ◽  
Temperature Dependence ◽  
Cell Mechanics ◽  
Force Microscopy ◽  
Atomic Force
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