A microscopic analysis of the effects of root surface scaling with different power parameters of er,cr:YSGG laser

2015 ◽  
Vol 78 (6) ◽  
pp. 529-535 ◽  
Author(s):  
Guilherme José Pimentel Lopes de Oliveira ◽  
Mariana Aline Cominotte ◽  
Tamara PÁdua Pereira Beraldo ◽  
José Eduardo Cezar Sampaio ◽  
Rosemary Adriana Chiérici Marcantonio
Keyword(s):  
Microscopic Analysis ◽  
Root Surface ◽  
Surface Scaling

scholarly journals Granular Size Effect of Clinoptilolite on Maize Seedlings Growth

2010 ◽  
Vol 4 (1) ◽  
pp. 23-30 ◽  
Author(s):  
Alessandra Trinchera ◽  
Carlos Mario Rivera ◽  
Simona Rinaldi ◽  
Anna Salerno ◽  
Elvira Rea ◽  
...  
Keyword(s):  
Organic Fertilizer ◽  
Root Hairs ◽  
Microscopic Analysis ◽  
Root Surface ◽  
Limited Data ◽  
Root Cells ◽  
Enhanced Production ◽  
Growing Medium ◽  
Isolated Roots ◽  
Inert Substrate

Clinoptilolite has been successfully used in growing media for containerized horticultural and floricultural production. However, limited data exist on the effects and interaction between particle size and organic nutrient enrichment of the clinoptilolite. One granular (1-3 mm) and micronized (< 30 µm) clinoptilolite was added to quartz sand, an inert growing substrate, at two doses (0.1% and 3% v/v), without or with addition of wine vinasse as nutrient source at four concentrations (0 mgF×Lsubst-1, 10 mgF×Lsubst-1, 100 mgF×Lsubst-1 and 1000 mgF×Lsubst-1) to evaluate their effect on root growth for five days or the appearance of the second true leaf. Root mucigel was produced in zones where clinoptilolite particles adhered to the root surface. Microscopic analysis of isolated roots showed the increase of secondary roots and the proliferation of root hairs in maize treated with both micronized and granular clinoptilolite, with the contemporary production of root mucigel in zones where zeolite particles adhered to the root surface. It is hypothesized that the enhanced production of mucigel by root cells can favour not only the penetration of roots into the inert substrate, but also the solubilization of organic matter and nutrient availability, in particular when micronized clinoptilolite was present in the growing medium. Therefore, micronized clinoptilolite behaved as a sort of a “physical stimulant” for roots during seedlings, promoting, as a consequence, maize shoot development. Effectively, the highest increase in shoot growth was observed at the highest dose (3% v/v) of micronized zeolite, with the optimal rate of organic fertilizer (100 mgF×Lsubst-1).

Comparing fibroblast attachment in root surface scaling with Er, Cr:YSGG laser versus ultrasonic scaler: A SEM study

2017 ◽  
Vol 80 (8) ◽  
pp. 917-922
Author(s):  
Reza Fekrazad ◽  
Ghogha Lotfi ◽  
Mohammad Harandi ◽  
Sara Ayremlou ◽  
Katayoun A. M. Kalhori ◽  
...  
Keyword(s):  
Root Surface ◽  
Ultrasonic Scaler ◽  
Sem Study ◽  
Fibroblast Attachment ◽  
Surface Scaling

scholarly journals Suppression of Damping-Off Disease in Host Plants by the Rhizoplane Bacterium Lysobacter sp. Strain SB-K88 Is Linked to Plant Colonization and Antibiosis against Soilborne Peronosporomycetes

2005 ◽  
Vol 71 (7) ◽  
pp. 3786-3796 ◽  
Author(s):  
Md. Tofazzal Islam ◽  
Yasuyuki Hashidoko ◽  
Abhinandan Deora ◽  
Toshiaki Ito ◽  
Satoshi Tahara
Keyword(s):  
Sugar Beet ◽  
Microscopic Analysis ◽  
Inhibitory Effect ◽  
Root Surface ◽  
Plant Colonization ◽  
Damping Off ◽  
Electron Microscopic ◽  
Aphanomyces Cochlioides ◽  
Direct Inhibitory Effect ◽  
A Cell

ABSTRACT We previously demonstrated that xanthobaccin A from the rhizoplane bacterium Lysobacter sp. strain SB-K88 suppresses damping-off disease caused by Pythium sp. in sugar beet. In this study we focused on modes of Lysobacter sp. strain SB-K88 root colonization and antibiosis of the bacterium against Aphanomyces cochlioides, a pathogen of damping-off disease. Scanning electron microscopic analysis of 2-week-old sugar beet seedlings from seeds previously inoculated with SB-K88 revealed dense colonization on the root surfaces and a characteristic perpendicular pattern of Lysobacter colonization possibly generated via development of polar, brush-like fimbriae. In colonized regions a semitransparent film apparently enveloping the root and microcolonies were observed on the root surface. This Lysobacter strain also efficiently colonized the roots of several plants, including spinach, tomato, Arabidopsis thaliana, and Amaranthus gangeticus. Plants grown from both sugar beet and spinach seeds that were previously treated with Lysobacter sp. strain SB-K88 displayed significant resistance to the damping-off disease triggered by A. cochlioides. Interestingly, zoospores of A. cochlioides became immotile within 1 min after exposure to a SB-K88 cell suspension, a cell-free supernatant of SB-K88, or pure xanthobaccin A (MIC, 0.01 μg/ml). In all cases, lysis followed within 30 min in the presence of the inhibiting factor(s). Our data indicate that Lysobacter sp. strain SB-K88 has a direct inhibitory effect on A. cochlioides, suppressing damping-off disease. Furthermore, this inhibitory effect of Lysobacter sp. strain SB-K88 is likely due to a combination of antibiosis and characteristic biofilm formation at the rhizoplane of the host plant.

Comparing the effects of root surface scaling with ultrasound instruments and Er,Cr:YSGG laser

2007 ◽  
Vol 23 (3) ◽  
pp. 283-287 ◽  
Author(s):  
Zohre Tabibzadeh Noori ◽  
Reza Fekrazad ◽  
Behnam Eslami ◽  
Ardavan Etemadi ◽  
Shabnam Khosravi ◽  
...  
Keyword(s):  
Root Surface ◽  
Surface Scaling

Evaluation of the topical effect of alendronate on the root surface of extracted and replanted teeth. Microscopic analysis on rats' teeth

2006 ◽  
Vol 22 (1) ◽  
pp. 30-35 ◽  
Author(s):  
Adriana Lustosa-Pereira ◽  
Roberto Brandao Garcia ◽  
Ivaldo Gomes de Moraes ◽  
Norberti Bernardineli ◽  
Clovis Monteiro Bramante ◽  
...  
Keyword(s):  
Microscopic Analysis ◽  
Root Surface

scholarly journals Comparing the effects of root surface scaling with ultrasound instruments and Er,Cr:YSGG laser

2007 ◽  
Vol 23 (3) ◽  
pp. 289-289
Author(s):  
Zohre Tabibzadeh Noori ◽  
Reza Fekrazad ◽  
Behnam Eslami ◽  
Ardavan Etemadi ◽  
Shabnam Khosravi ◽  
...  
Keyword(s):  
Root Surface ◽  
Surface Scaling

Preparatory Procedures for Electron Microscopic Analysis at the Molecular Level of Resolution

1978 ◽  
Vol 36 (3) ◽  
pp. 516-520
Author(s):  
F.J. Sjostrand
Keyword(s):  
Electron Microscope ◽  
Molecular Level ◽  
Microscopic Analysis ◽  
Resolving Power ◽  
Cellular Structures ◽  
Electron Microscopic ◽  
Systematic Analysis ◽  
Technical Developments ◽  
Thin Sectioning ◽  
Obvious Reason

In the 1940's and 1950's electron microscopy conferences were attended with everybody interested in learning about the latest technical developments for one very obvious reason. There was the electron microscope with its outstanding performance but nobody could make very much use of it because we were lacking proper techniques to prepare biological specimens. The development of the thin sectioning technique with its perfectioning in 1952 changed the situation and systematic analysis of the structure of cells could now be pursued. Since then electron microscopists have in general become satisfied with the level of resolution at which cellular structures can be analyzed when applying this technique. There has been little interest in trying to push the limit of resolution closer to that determined by the resolving power of the electron microscope.

Electron Microscopic Analysis of Myonecrosis Induced by a Pure Component Isolated From Rattlesnake Venom

1976 ◽  
Vol 34 ◽  
pp. 292-293
Author(s):  
Charlotte L. Ownby ◽  
David Cameron ◽  
Anthony T. Tu
Keyword(s):  
Gel Filtration ◽  
Microscopic Analysis ◽  
The United States ◽  
Exchange Chromatography ◽  
Pure Component ◽  
Electron Microscopic Level ◽  
Electron Microscopic ◽  
Mouse Muscle ◽  
Major Health Problem ◽  
Rattlesnake Venom

In the United States the major health problem resulting from snakebite poisoning is local tissue damage, i.e. hemorrhage and myonecrosis. Since commercial antivenin does not usually prevent such damage to tissue, a more effective treatment of snakebite-induced myonecrosis is needed. To aid in the development of such a treatment the pathogenesis of myonecrosis induced by a pure component of rattlesnake venom was studied at the electron microscopic level.The pure component, a small (4,300 mol. wt.), basic (isoelectric point of 9.6) protein, was isolated from crude prairie rattlesnake (Crotalus viridis viridis) venom by gel filtration (Sephadex G-50) followed by cation exchange chromatography (Sephadex C-25), and shown to be pure by electrophoresis. Selection of the myotoxic component was based on light microscopic observations of injected mouse muscle.

Molecular and Microscopic Analysis of Altered Gene Expression in Transgenic and Gene Targeted Mice

1996 ◽  
Vol 54 ◽  
pp. 12-13
Author(s):  
W. K. Jones ◽  
J. Robbins
Keyword(s):  
Gene Expression ◽  
Pulmonary Veins ◽  
Microscopic Analysis ◽  
Mouse Tissue ◽  
Adult Heart ◽  
Heavy Chains ◽  
Altered Gene Expression ◽  
Altered Gene ◽  
Pulmonary Myocardium

Two myosin heavy chains (MyHC) are expressed in the mammalian heart and are differentially regulated during development. In the mouse, the α-MyHC is expressed constitutively in the atrium. At birth, the β-MyHC is downregulated and replaced by the α-MyHC, which is the sole cardiac MyHC isoform in the adult heart. We have employed transgenic and gene-targeting methodologies to study the regulation of cardiac MyHC gene expression and the functional and developmental consequences of altered α-MyHC expression in the mouse.We previously characterized an α-MyHC promoter capable of driving tissue-specific and developmentally correct expression of a CAT (chloramphenicol acetyltransferase) marker in the mouse. Tissue surveys detected a small amount of CAT activity in the lung (Fig. 1a). The results of in situ hybridization analyses indicated that the pattern of CAT transcript in the adult heart (Fig. 1b, top panel) is the same as that of α-MyHC (Fig. 1b, lower panel). The α-MyHC gene is expressed in a layer of cardiac muscle (pulmonary myocardium) associated with the pulmonary veins (Fig. 1c). These studies extend our understanding of α-MyHC expression and delimit a third cardiac compartment.

Correlative video enhanced differential interference contrast light microscopy (VDIC)-LM), low-voltage high-resolution SEM (LV-HR-SEM), and high-voltage electron microscopy (HVEM) in the observation of gold-labelled surface antigens and the subjacent cytoskeletal matrix

1989 ◽  
Vol 47 ◽  
pp. 904-905
Author(s):  
Ralph M. Albrecht ◽  
Scott R. Simmons ◽  
Marek Malecki
Keyword(s):  
High Resolution ◽  
Light Microscopy ◽  
Low Voltage ◽  
Microscopic Analysis ◽  
Cell Labelling ◽  
Video Technology ◽  
Electron Microscopic ◽  
Image Capture ◽  
High Voltage Electron Microscopy ◽  
Fluorescence Studies

The development of video-enhanced light microscopy (LM) as well as associated image processing and analysis have significantly broadened the scope of investigations which can be undertaken using (LM). Interference/polarization based microscopies can provide high resolution and higher levels of “detectability” especially in unstained living systems. Confocal light microscopy also holds the promise of further improvements in resolution, fluorescence studies, and 3 dimensional reconstruction. Video technology now provides, among other things, a means to detect differences in contrast difficult to detect with the human eye; furthermore, computerized image capture, processing, and analysis can be used to enhance features of interest, average images, subtract background, and provide a quantitative basis to studies of cells, cell features, cell labelling, and so forth. Improvements in video technology, image capture, and cost-effective computer image analysis/processing have contributed to the utility and potential of the various interference and confocal microscopic instrumentation.Electron microscopic technology has made advances as well. Microprocessor control and improved design have contributed to high resolution SEMs which have imaging capability at the molecular level and can operate at a range of accelerating voltages starting at 1KV. Improvements have also been seen in the HVEM and IVEM transmission instruments. As a whole, these advances in LM and EM microscopic technology provide the biologist with an array of information on structure, composition, and function which can be obtained from a single specimen. Corrrelative light microscopic analysis permits examination of living specimens and is critical where the “history” of a cell, cellular components, or labels needs to be known up to the time of chemical or physical fixation. Features such as cytoskeletal elements or gold label as small as 0.01 μm, well below the 0.2 μm limits of LM resolution, can be “detected” and their movement followed by VDIC-LM. Appropriate identification and preparation can then lead to the examination of surface detail and surface label with stereo LV-HR-SEM. Increasing the KV in the HR-SEM while viewing uncoated or thinly coated specimens can provide information from beneath the surface as well as increasing Z contrast so that positive identification of surface and subsurface colloidal gold or other heavy metal labelled/stained material is possible. Further examination of the same cells using stereo HVEM or IVEM provides information on internal ultrastructure and on the relationship of labelled material to cytoskeletal or organellar distribution, A wide variety of investigations can benefit from this correlative approach and a number of instrumentational configurations and preparative pathways can be tailored for the particular study. For a surprisingly small investment in time and technique, it is often possible to clear ambiguities or questions that arise when a finding is presented in the context of only one modality.

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