Collagen arrangement in space of Disse correlates with fluid flow in normal and cirrhotic rat livers

2014 ◽  
Vol 78 (2) ◽  
pp. 187-193 ◽  
Author(s):  
Raksawan Poonkhum ◽  
Udomsri Showpittapornchai ◽  
Wisuit Pradidarcheep
Keyword(s):  
Fluid Flow ◽  
Space Of Disse ◽  
Rat Livers

An ultrastructural study on the role of Kupffer cells in the process of infection by Plasmodium berghei sporozoites in rats

Parasitology ◽  
1983 ◽  
Vol 86 (2) ◽  
pp. 231-242 ◽  
Author(s):  
J. F. G. M. Meis ◽  
J. P. Verhave ◽  
P. H. K. Jap ◽  
J. H. E. Th. Meuwissen
Keyword(s):  
Kupffer Cells ◽  
Plasmodium Berghei ◽  
Brown Norway ◽  
Liver Sinusoids ◽  
Transmission Electron ◽  
Space Of Disse ◽  
Rat Livers ◽  
Norway Rats

SUMMARYThe interactions in vivo between Plasmodium berghei sporozoites and Kupffer cells in rat livers were studied by transmission electron microscopy. By 10 and 15 min after inoculation, sporozoites were both free in the liver sinusoids and inside endocytotic vacuoles of the Kupffer cells. The latter cells were very active in phagocytosing sporozoites, bacteria and red blood cells. The sporozoites retained their integrity inside the endocytotic vacuoles and no signs of lysosomal digestion were observed. Sporozoites seen within endocytotic vacuoles 1 h after inoculation were still morphologically intact, although bristle-coated vesicles fused with the vacuole membrane. Evidence is presented which suggests that Kupffer cells transport sporozoites towards the space of Disse and adjacent hepatocytes. No sporozoites were seen to penetrate an endothelial cell or its narrow fenestrae. It is proposed that Kupffer cell passage, rather than gaps in the sinusoidal lining, represents the normal route that sporozoites take to circumvent the endothelial barrier. The localization of exo-erythrocytic forms was made easier by the use of Brown Norway rats in which many more parasites develop than in the Wistar rats. The distribution pattern of the parasites was found to be mainly around the ‘periportal’ zones of the acini of liver tissue.

Carbon monoxide overproduced by heme oxygenase-1 causes a reduction of vascular resistance in perfused rat liver

1999 ◽  
Vol 277 (5) ◽  
pp. G1088-G1096 ◽  
Author(s):  
Yoshiyuki Wakabayashi ◽  
Rina Takamiya ◽  
Akira Mizuki ◽  
Takanori Kyokane ◽  
Nobuhito Goda ◽  
...  
Keyword(s):  
Carbon Monoxide ◽  
Vascular Resistance ◽  
Heme Oxygenase ◽  
Heme Oxygenase 1 ◽  
Basal Resistance ◽  
Endothelin 1 ◽  
Vascular Hyporeactivity ◽  
Space Of Disse ◽  
The Relationship ◽  
Rat Livers

This study aimed to examine whether livers overexpressing heme oxygenase (HO)-1 could alter the vascular resistance through the vasorelaxing action of carbon monoxide (CO). The relationship among HO-1 expression, CO generation, and the vascular resistance was assessed in perfused rat livers pretreated with hemin, an inducer of HO-1. At 18 h after the hemin treatment, livers displayed marked increases in HO-1 expression in hepatocytes and venous CO flux and a reduction of the basal resistance. The reduction of the resistance in hemin-treated livers was canceled by administration of oxyhemoglobin, a reagent trapping both CO and nitric oxide (NO), but not by methemoglobin, which captures NO but not CO. Liposome-encapsulated oxyhemoglobin, which cannot access the space of Disse, did not cause vasoconstriction. Furthermore, these livers became less sensitive to endothelin-1, a vasoconstrictive peptide, than the untreated controls through mechanisms involving CO. On the other hand, at 12 or 24 h after the treatment when the HO-1 induction was not accompanied by CO overproduction, neither a decrease in the basal resistance nor vascular hyporeactivity to endothelin-1 was observed. These results suggest that CO overproduced in the extrasinusoidal compartment is a determinant of the HO-1-mediated vasorelaxation in the liver.

Ultrastructural changes in isolated rat liver perfused with cyclic heptapeptide toxins from norwegian freshwater cyanobacteria (blue-green algae)

1987 ◽  
Vol 45 ◽  
pp. 858-859
Author(s):  
A.S. Dabholkar ◽  
W.W. Carmichael ◽  
K. Berg ◽  
J. Wyman
Keyword(s):  
Ultrastructural Changes ◽  
Sprague Dawley ◽  
Isolated Rat Liver ◽  
Blue Green Algae ◽  
Sprague Dawley Rats ◽  
Cyclic Heptapeptide ◽  
Intracellular Changes ◽  
Oscillatoria Agardhii ◽  
Rat Livers ◽  
Isolated Rat

Intracellular changes in the hepatocytes of isolated rat livers perfused with cyclic heptapeptide toxins are described. The toxins used are 1) -Ala-Leu- β-methyl isoAsp-Arg-ADDA-isoGlu-mdha (M.W. 944) from Microcystis aeruginosa- Lake Akersvatn, Norway; 2) -Ala-Arg-isoAsp-Arg-ADDA-isoGlu-mdha (M.W. 1023) from Oscillatoria agardhii var. - Lake Kolbatnvatn, Norway; 3) -Ala-Arg-isoAsp-Arg-ADDA-isoGlu-dha (M.W. 1009) from Oscillatoria agardhii var. isothrix - Lake Froylandsvatn, Norway. Approximate LD intraperitoneal mouse for the toxins is 50, 500 and 1000 μg/kg respectively.Livers were removed from male Sprague Dawley rats and perfused for 15 min with a blood-free perfusate (50 ml) followed by 60 min with perfusate containing i) 25, 50, or 200 μg of M. aeruginosa toxin ii) 50, 250, 500 or 1000 μg of O. agardhii var. toxin and iii) 1000, 2000, 2500 or 5000 μg of O. agardhii var. isothrix toxin. Control livers were perfused for 75 min with the blood-free perfusate.

Short stroke linear motor controls fluid flow

1998 ◽  
Vol 08 (PR2) ◽  
pp. Pr2-805-Pr2-808
Author(s):  
A. Basak
Keyword(s):  
Fluid Flow ◽  
Linear Motor ◽  
Motor Controls
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