scholarly journals Mesoporous iron oxide nanoparticles prepared by polyacrylic acid etching and their application in gene delivery to mesenchymal stem cells

2013 ◽  
Vol 76 (9) ◽  
pp. 936-941 ◽  
Author(s):  
Binrui Cao ◽  
Penghe Qiu ◽  
Chuanbin Mao
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Iron Oxide ◽  
Gene Delivery ◽  
Iron Oxide Nanoparticles ◽  
Polyacrylic Acid ◽  
Acid Etching ◽  
Oxide Nanoparticles

Uniform iron oxide nanoparticles reduce the required amount of polyethylenimine in the gene delivery to mesenchymal stem cells

2021 ◽  
Author(s):  
Donghang Xu ◽  
Yuanqin Su ◽  
Qianhao Xu ◽  
Ting Huang ◽  
Zhilan Chen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Iron Oxide ◽  
Gene Delivery ◽  
Charge Density ◽  
Iron Oxide Nanoparticles ◽  
Electrostatic Interactions ◽  
Positive Charge ◽  
Viral Gene ◽  
Oxide Nanoparticles

Abstract Cationic polyethylenimine (PEI) is regarded as the “golden standard” of non-viral gene vectors. However, the superiority of PEI with high positive charge density also induces its major drawback of cytotoxicity, which restricts its application for an effective and safe gene delivery to stem cells. To redress this shortcoming, herein, a magnetic gene complex containing uniform iron oxide nanoparticles (UIONPs), plasmid DNA, and free PEI is prepared through electrostatic interactions for the gene delivery to bone marrow-derived mesenchymal stem cells (BM-MSCs). Results show that UIONPs dramatically promote the gene delivery to BM-MSCs using the assistance of magnetic force. In addition, decreasing the free PEI nitrogen to DNA phosphate (N/P) ratio from 10 to 6 has little adverse impact on the transgene expression levels (over 300 times than that of PEI alone at the N/P ratio of 6) and significantly reduces the cytotoxicity to BM-MSCs. Further investigations confirmed that the decrease of free PEI has little influence on the cellular uptake after applying external magnetic forces, but that the reduced positive charge density decreases the cytotoxicity. The present study demonstrates that the magnetic gene delivery not only contributes to the enhanced gene delivery efficiency but also helps to reduce required amount of PEI, providing a potential strategy for an efficient and safe gene delivery to stem cells.

Uniformly sized iron oxide nanoparticles for efficient gene delivery to mesenchymal stem cells

2018 ◽  
Vol 552 (1-2) ◽  
pp. 443-452 ◽  
Author(s):  
Qianhao Xu ◽  
Tianyuan Zhang ◽  
Qiyue Wang ◽  
Xinchi Jiang ◽  
Ai Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Iron Oxide ◽  
Gene Delivery ◽  
Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Efficient Gene

scholarly journals Iron oxide nanoparticles promote the migration of mesenchymal stem cells to injury sites

2019 ◽  
Vol Volume 14 ◽  
pp. 573-589 ◽  
Author(s):  
Xiuying Li ◽  
Zhenhong Wei ◽  
Huiying Lv ◽  
Liya Wu ◽  
Yingnan Cui ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles

Dextran-coated iron oxide nanoparticles turn protumor mesenchymal stem cells (MSCs) into antitumor MSCs

RSC Advances ◽  
2016 ◽  
Vol 6 (51) ◽  
pp. 45553-45561 ◽  
Author(s):  
Tsai-Hua Chung ◽  
Chia-Chu Hsieh ◽  
Jong-Kai Hsiao ◽  
Szu-Chun Hsu ◽  
Ming Yao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Tumor Tropism

dex-IO NPs can activate the antitumor mechanism (tumor tropism) but inactivate protumor mechanisms to transform protumor MSCs (pT-MSCs) into antitumor MSCs (aT-MSCs).

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