Comparative study of hydrophilic and hydrophobic ionic liquids for observing cultured human cells by scanning electron microscopy

2011 ◽  
Vol 74 (12) ◽  
pp. 1104-1108 ◽  
Author(s):  
Yasuhito Ishigaki ◽  
Yuka Nakamura ◽  
Teruaki Takehara ◽  
Takayuki Kurihara ◽  
Hironori Koga ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Ionic Liquids ◽  
Comparative Study ◽  
Human Cells ◽  
Cultured Human Cells ◽  
Hydrophobic Ionic Liquids ◽  
Scanning Electron

scholarly journals Scanning Electron Microscopy Analysis of the Anterior Capsulotomy Edge: A Comparative Study between Femtosecond Laser-Assisted Capsulotomy and Manual Capsulorhexis

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Daniele Tognetto ◽  
Chiara De Giacinto ◽  
Alberto Armando Perrotta ◽  
Tommaso Candian ◽  
Alessandro Bova ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Femtosecond Laser ◽  
Comparative Study ◽  
Surface Irregularity ◽  
Significant Difference ◽  
Group 2 ◽  
Cut Surface ◽  
Scanning Electron ◽  
Group 1

Purpose. To compare the capsule edges ultrastructure obtained by two femtosecond laser-assisted cataract surgery (FLACS) platforms and manual continuous curvilinear capsulorhexis (CCC) using scanning electron microscopy (SEM). Setting. Eye Clinic, University of Trieste, Italy. Design. Experimental comparative study. Methods. 150 anterior capsules were collected and divided into three groups as follows: Group 1 (50 capsules) obtained with manual CCC, Groups 2 and 3 (each with 50 capsules) obtained with the Catalys Laser and the LenSx Laser, respectively. All samples were imaged by means of SEM and regularity of the cut surface, and thickness of the capsule edge were evaluated and compared. Results. All femtosecond laser (FSL) capsules were perfectly circular, whereas some alteration of the circular shape was observed in the manual ones. Group 1 showed a smooth and regular capsule edge without any surface irregularity, conversely Groups 2 and 3 showed postage-stamp perforations on the capsule edge. The cut surface irregularity value in Group 2 was 1.4 ± 0.63, while it was 0.7 ± 0.49 in Group 3 (p<0.05). Group 1 had a significantly lower thickness of the capsule edge than the FSL groups (p<0.05). No statistically significant difference in the capsule edge thickness between the FSL groups was found (p=0.244). Conclusions. Despite the presence of slight cut surface irregularities, both FSL capsulotomies showed a better geometry and circularity than the manual ones. Capsulotomy specimens obtained using both FSL capsulotomies showed laser-induced alterations of the capsule edge when compared with smooth and regular edges obtained using manual CCC.

A Comparative Study of Trichomes in Angophora Cav. And Eucalyptus L'hérit. - A Question of Homology

1984 ◽  
Vol 32 (5) ◽  
pp. 561 ◽  
Author(s):  
PY Ladiges
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Comparative Study ◽  
Light Microscopy ◽  
Evolutionary Trends ◽  
Oil Glands ◽  
Ancestral Condition ◽  
Scanning Electron

The trichomes of Angophora and Eucalyptus are illustrated from scanning electron microscopy and light microscopy, and evolutionary trends are discussed. Bristle glands of Angophora and Eucalyptus subgen. Blakella and Corymbia are emergent oil glands of varying lengths. Emergent oil glands occur in all other Eucalyptus subgenera but they are most conspicuous in Blakella, Corymbia and Angophora, in which they are characterized by four cap cells each ornamented with micropapillae. Hairs in Angophora are unique, being multicellular; they are also uniseriate and scattered on the epidermis. In contrast, hairs in Eucalyptus are simple extensions, short or long, of the cells on the sides of or the cap cells of the emergent oil glands, and they are not homologous with those of Angophora. Eucalyptus setosa (subgen. Blakella) and E. brockwayi (subgen. Symphyomyrtus) are two exceptions, having unicellular hairs on the epidermis, not associated with oil glands. It is suggested that this is an ancestral condition (or secondary reversal to it).

Comparative Study of Field Emission-Scanning Electron Microscopy and Atomic Force Microscopy to Assess Self-assembled Monolayer Coverage on Any Type of Substrate

1999 ◽  
Vol 5 (6) ◽  
pp. 413-419 ◽  
Author(s):  
Bernardo R.A. Neves ◽  
Michael E. Salmon ◽  
Phillip E. Russell ◽  
E. Barry Troughton
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Atomic Force Microscopy ◽  
Comparative Study ◽  
Field Emission ◽  
Self Assembled Monolayer ◽  
Force Microscopy ◽  
Atomic Force ◽  
Self Assembled ◽  
Scanning Electron

Abstract: In this work, we show how field emission–scanning electron microscopy (FE-SEM) can be a useful tool for the study of self-assembled monolayer systems. We have carried out a comparative study using FE-SEM and atomic force microscopy (AFM) to assess the morphology and coverage of self-assembled monolayers (SAM) on different substrates. The results show that FE-SEM images present the same qualitative information obtained by AFM images when the SAM is deposited on a smooth substrate (e.g., mica). Further experiments with rough substrates (e.g., Al grains on glass) show that FE-SEM is capable of unambiguously identifying SAMs on any type of substrate, whereas AFM has significant difficulties in identifying SAMs on rough surfaces.

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