Morphological changes in vero cells postinfection with dengue virus type‐2

2011 ◽  
Vol 74 (4) ◽  
pp. 314-319 ◽  
Author(s):  
Seema Zargar ◽  
Tanveer A. Wani ◽  
S. K. Jain
Keyword(s):  
Dengue Virus ◽  
Virus Type ◽  
Morphological Changes ◽  
Vero Cells ◽  
Dengue Virus Type 2

scholarly journals Zinc accelerates dengue virus type 2-induced apoptosis in Vero cells

FEBS Letters ◽  
2002 ◽  
Vol 524 (1-3) ◽  
pp. 20-24 ◽  
Author(s):  
Norazizah Shafee ◽  
Sazaly AbuBakar
Keyword(s):  
Dengue Virus ◽  
Virus Type ◽  
Vero Cells ◽  
Induced Apoptosis ◽  
Dengue Virus Type 2

scholarly journals Chloroquine Inhibits Dengue Virus Type 2 Replication in Vero Cells but Not in C6/36 Cells

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Kleber Juvenal Silva Farias ◽  
Paula Renata Lima Machado ◽  
Benedito Antônio Lopes da Fonseca
Keyword(s):  
Dengue Virus ◽  
Virus Type ◽  
Mammalian Cells ◽  
Plaque Assay ◽  
Antiviral Drug ◽  
Vero Cells ◽  
Significant Difference ◽  
Dengue Virus Type 2

Dengue viruses are the most important arthropod-borne viruses in terms of morbidity and mortality in the world. Since there is no dengue vaccine available for human use, we have set out to investigate the use of chloroquine as an antiviral drug against dengue. Chloroquine, an amine acidotropic drug known to affect intracellular exocytic pathways by increasing endosomal pH, was used in the in vitro treatment of Vero and C6/36 cells infected with dengue virus type 2 (DENV-2). Real-time RT-PCR and plaque assays were used to quantify the DENV-2 load in infected Vero and C6/36 cells after chloroquine treatment. Our results showed that a dose of 50 μg/ml of chloroquine was not toxic to the cells and induced a statistically significant inhibition of virus production in infected Vero cells when compared to untreated cells. In C6/36 cells, chloroquine does not induce a statistically significant difference in viral replication when compared to untreated cells, showing that this virus uses an unlikely pathway of penetration in these cells, and results were also confirmed by the plaque assay (PFU). These data suggest that the inhibition of virus infection induced by chloroquine is due to interference with acidic vesicles in mammalian cells.

scholarly journals Flavone Enhances Dengue Virus Type-2 (NGC Strain) Infectivity and Replication in Vero Cells

Molecules ◽  
2012 ◽  
Vol 17 (3) ◽  
pp. 2437-2445 ◽  
Author(s):  
Keivan Zandi ◽  
Rafidah Lani ◽  
Pooi-Fong Wong ◽  
Boon-Teong Teoh ◽  
Sing-Sin Sam ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Virus Type ◽  
Vero Cells ◽  
Dengue Virus Type 2

Antibodies that block virus attachment to vero cells are a major component of the human neutralizing antibody response against dengue virus type 2

1995 ◽  
Vol 45 (4) ◽  
pp. 451-461 ◽  
Author(s):  
Run-Tao He ◽  
Songli Wang ◽  
Robert Anderson ◽  
Bruce L. Innis ◽  
Ananda Nisalak ◽  
...  
Keyword(s):  
Antibody Response ◽  
Dengue Virus ◽  
Virus Type ◽  
Neutralizing Antibody ◽  
Vero Cells ◽  
Virus Attachment ◽  
Dengue Virus Type 2

scholarly journals Brazilian Dengue Virus Type 2-Associated Renal Involvement in a Murine Model: Outcomes after Infection by Two Lineages of the Asian/American Genotype

Pathogens ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1084
Author(s):  
Fernanda Cunha Jácome ◽  
Gabriela Cardoso Caldas ◽  
Arthur da Costa Rasinhas ◽  
Ana Luisa Teixeira de Almeida ◽  
Daniel Dias Coutinho de Souza ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Virus Type ◽  
Asian American ◽  
Murine Model ◽  
Morphological Changes ◽  
Renal Involvement ◽  
Denv Infection ◽  
Severe Dengue ◽  
Dengue Virus Type 2

Dengue virus type 2 (DENV-2) is, traditionally, the most studied serotype due to its association with explosive outbreaks and severe cases. In Brazil, almost 20 years after the first introduction in the 1990s, a new lineage (Lineage II) of the DENV-2 Asian/American genotype emerged and caused an epidemic with severe cases and hospitalizations. Severe dengue includes multiple organ failure, and renal involvement can be potentially related to increased mortality. In order to better understand the role of DENV infection in renal injury, here we aimed to investigate the outcomes of infection with two distinct lineages of DENV-2 Asian/American genotype in the kidney of a murine model. BALB/c mice were infected with Lineages I and II and tissues were submitted to histopathology, immunohistochemistry, histomorphometry and ultrastructural analysis. Blood urea nitrogen (BUN) was detected in blood sample accessed by cardiac puncture. A tendency in kidney weight increase was observed in mice infected with both lineages, but urea levels, on average, were increased only in mice infected with Lineage II. The DENV antigen was detected in the tissue of mice infected with Lineage II and morphological changes were similar to those observed in human dengue cases. Furthermore, the parameters such as organ weight, urea levels and morphometric analysis, showed significant differences between the two lineages in the infected BALB/c, which was demonstrated to be a suitable experimental model for dengue pathophysiology studies in kidneys.

scholarly journals ANTI-DENGUE TYPE 2 VIRUS ACTIVITIES OF ZINC (II) COMPLEX COMPOUNDS WITH 2-(2,4 -DIHYDROXYPHENYL)-3,5,7-TRIHYDROXYCROMEN-4-ONE LIGANDS IN VERO CELLS

2019 ◽  
Vol 7 (5) ◽  
pp. 105 ◽  
Author(s):  
Teguh Hari Sucipto ◽  
Harsasi Setyawati ◽  
Siti Churrotin ◽  
Ilham Harlan Amarullah ◽  
Sri Sumarsih ◽  
...  
Keyword(s):  
Antiviral Activity ◽  
Dengue Virus ◽  
Virus Type ◽  
Complex Compound ◽  
Vero Cells ◽  
Complex Compounds ◽  
Dengue Type 2 Virus ◽  
Dengue Virus Type 2 ◽  
Metal Ligand

Dengue virus (DENV) is a disease that is transmitted through Aedes aegypti and Aedes albopictus mosquitoes, and is spread in tropical and sub-tropical regions. Now, dengue or antiviral vaccines for humans do not yet exist, but there are great efforts to achieve this goal. Complex compounds are reported to fungicidal, bactericidal and antiviral activity. Antiviral activity against DENV is an important alternative to the characterization and development of drugs candidate. The purpose of this study was to study zinc(II) compounds with 2-(2,4-dihydroxyphenyl)-3,5,7-trihydroxycromen-4-one ligand on DENV-2 replication in Vero cells. Vero cell lines (African green monkey kidney) was used in this study, maintained and propagated in Minimum Essential Eagle Medium containing 10% fetal bovine serum at 37°C in 5% CO2. The activity of dengue virus was carried out by enzyme-immunosorbent assay (ELISA) method and CellTiter96® Non-Radioactive Proliferation. The value of activity inhibition (IC50) of complex compounds with variations of mol metal: ligand 1:2, 1:3, and 1:4 against dengue virus type 2 (DENV2) was 2.44 μg/ml, 2.75 μg/ml, respectively and 2.00 μg/ml, also the toxicity value (CC50) of complex compounds with variation mol metal: ligand 1:4 for Vero cells is 3.59 μg/ml. The results of this study were indicate that these properties have been shown to inhibit anti-dengue type 2 virus (DENV-2), but are also toxic in Vero cells. Including previous study about complex compound interaction with dengue virus type 2 activity, Zn(II) more reactive compound then Cu(II), and Co(II). The comparison with Cu(II) complex compound, it has been revealed that Co(II) and Zn(II) is more toxic, was found to be nontoxic to human erythrocyte cells even at a concentration of 500 μg/ml.

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