A study of hydrogenated carbon fibers by scanning electron microscopy and confocal laser scanning microscopy

2009 ◽  
Vol 72 (6) ◽  
pp. 447-453
Author(s):  
Antonio Madroñero De La Cal ◽  
Juan Aguado-Serrano ◽  
Maria Luisa Rojas-Cervantes ◽  
Elena V. Rosa Adame ◽  
Belen Sarmiento Marron ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Confocal Laser Scanning Microscopy ◽  
Carbon Fibers ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Scanning Microscopy ◽  
Confocal Laser ◽  
Scanning Electron

scholarly journals Cytogenetic Characterization of the TM4 Mouse Sertoli Cell Line. II. Chromosome Microdissection, FISH, Scanning Electron Microscopy, and Confocal Laser Scanning Microscopy

2015 ◽  
Vol 147 (2-3) ◽  
pp. 135-143 ◽  
Author(s):  
Michael Schmid ◽  
Martina Guttenbach ◽  
Claus Steinlein ◽  
Gerhard Wanner ◽  
Andreas Houben
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Confocal Laser Scanning Microscopy ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Scanning Microscopy ◽  
Confocal Laser ◽  
Marker Chromosomes ◽  
Scanning Electron

The chromosomes and interphase cell nuclei of the permanent mouse Sertoli cell line TM4 were examined by chromosome microdissection, FISH, scanning electron microscopy, and confocal laser scanning microscopy. The already known marker chromosomes m1-m5 were confirmed, and 2 new large marker chromosomes m6 and m7 were characterized. The minute heterochromatic marker chromosomes m4 and m5 were microdissected and their DNA amplified by DOP-PCR. FISH of this DNA probe on TM4 metaphase chromosomes demonstrated that the m4 and m5 marker chromosomes have derived from the centromeric regions of normal telocentric mouse chromosomes. Ectopic pairing of the m4 and m5 marker chromosomes with the centromeric region of any of the other chromosomes (centromeric associations) was apparent in ∼60% of the metaphases. Scanning electron microscopy revealed DNA-protein bridges connecting the centromeric regions of normal chromosomes and the associated m4 and m5 marker chromosomes. Interphase cell nuclei of TM4 Sertoli cells did not exhibit the characteristic morphology of Sertoli cells in the testes of adult mice as shown by fluorescence microscopy and confocal laser scanning microscopy.

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