Exploring molecular motors and switches at the single-molecule level

2004 ◽  
Vol 65 (4-5) ◽  
pp. 194-204 ◽  
Author(s):  
M. Capitanio ◽  
F. Vanzi ◽  
C. Broggio ◽  
R. Cicchi ◽  
D. Normanno ◽  
...  
Keyword(s):  
Single Molecule ◽  
Molecular Motors ◽  
Single Molecule Level

Molecular Motors: Force and Movement Generated by Single Myosin II Molecules

Physiology ◽  
2002 ◽  
Vol 17 (5) ◽  
pp. 213-218 ◽  
Author(s):  
Caspar Rüegg ◽  
Claudia Veigel ◽  
Justin E. Molloy ◽  
Stephan Schmitz ◽  
John C. Sparrow ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Single Molecule ◽  
Molecular Motors ◽  
Molecular Motor ◽  
Myosin Ii ◽  
Force Production ◽  
Myosin Isoforms ◽  
Single Molecule Level ◽  
Recent Developments ◽  
Muscle Myosin

Muscle myosin II is an ATP-driven, actin-based molecular motor. Recent developments in optical tweezers technology have made it possible to study movement and force production on the single-molecule level and to find out how different myosin isoforms may have adapted to their specific physiological roles.

scholarly journals Molecular motors: forty years of interdisciplinary research

2011 ◽  
Vol 22 (21) ◽  
pp. 3936-3939 ◽  
Author(s):  
James A. Spudich
Keyword(s):  
Single Molecule ◽  
Molecular Motors ◽  
Atp Hydrolysis ◽  
Molecular Motor ◽  
Single Molecule Level ◽  
In Vitro Motility ◽  
Nonmuscle Cell ◽  
Nonmuscle Cells ◽  
City Plan

A mere forty years ago it was unclear what motor molecules exist in cells that could be responsible for the variety of nonmuscle cell movements, including the “saltatory cytoplasmic particle movements” apparent by light microscopy. One wondered whether nonmuscle cells might have a myosin-like molecule, well known to investigators of muscle. Now we know that there are more than a hundred different molecular motors in eukaryotic cells that drive numerous biological processes and organize the cell's dynamic city plan. Furthermore, in vitro motility assays, taken to the single-molecule level using techniques of physics, have allowed detailed characterization of the processes by which motor molecules transduce the chemical energy of ATP hydrolysis into mechanical movement. Molecular motor research is now at an exciting threshold of being able to enter into the realm of clinical applications.

scholarly journals Nucleosome-remodelling machines and other molecular motors observed at the single-molecule level

FEBS Journal ◽  
2011 ◽  
Vol 278 (19) ◽  
pp. 3596-3607 ◽  
Author(s):  
Christophe Lavelle ◽  
Elise Praly ◽  
David Bensimon ◽  
Eric Le Cam ◽  
Vincent Croquette
Keyword(s):  
Single Molecule ◽  
Molecular Motors ◽  
Single Molecule Level

scholarly journals Energetic costs, precision, and efficiency of a biological motor in cargo transport

2017 ◽  
Author(s):  
Wonseok Hwang ◽  
Changbong Hyeon
Keyword(s):  
Single Molecule ◽  
Molecular Motors ◽  
High Speed ◽  
Uncertainty Relation ◽  
Energetic Cost ◽  
Minimal Amount ◽  
Single Molecule Level ◽  
Cargo Transport ◽  
Biological Motor ◽  
Cellular Condition

AbstractMolecular motors play key roles in organizing the interior of cells. An efficient motor in cargo transport would travel with a high speed and a minimal error in transport time (or distance) while consuming minimal amount of energy. The travel distance and its variance of motor are, however, physically constrained by energy consumption, the principle of which has recently been formulated into thethermodynamic uncertainty relation. Here, we reinterpret the uncertainty measure (𝒬) defined in the thermodynamic uncertainty relation such that a motor efficient in cargo transport is characterized with a small 𝒬. Analyses on the motility data from several types of molecular motors show that 𝒬 is a nonmonotic function of ATP concentration and load (f). For kinesin-1, 𝒬 is locally minimized at [ATP] ≈ 200μM andf≈ 4 pN. Remarkably, for the mutant with a longer neck-linker this local minimum vanishes, and the energetic cost to achieve the same precision as the wild-type increases significantly, which underscores the importance of molecular structure in transport properties. For the biological motors studied here, their value of 𝒬 is semi-optimized under the cellular condition ([ATP] ≈ 1 mM,f= 0 − 1 pN). We find that among the motors, kinesin-1 at single molecule level is the most efficient in cargo transport.

Single-molecule fluorescence to study molecular motors

2007 ◽  
Vol 40 (1) ◽  
pp. 87-111 ◽  
Author(s):  
Hyokeun Park ◽  
Erdal Toprak ◽  
Paul R. Selvin
Keyword(s):  
Fluorescence Imaging ◽  
Single Molecule ◽  
Molecular Motors ◽  
Atp Hydrolysis ◽  
Imaging Techniques ◽  
Single Molecule Fluorescence ◽  
New Techniques ◽  
Single Molecule Level ◽  
Living Organisms ◽  
Run Lengths

AbstractMolecular motors, which use energy from ATP hydrolysis to take nanometer-scale steps with run-lengths on the order of micrometers, have important roles in areas such as transport and mitosis in living organisms. New techniques have recently been developed to measure these small movements at the single-molecule level. In particular, fluorescence imaging has contributed to the accurate measurement of this tiny movement. We introduce three single-molecule fluorescence imaging techniques which can find the position of a fluorophore with accuracy in the range of a few nanometers. These techniques are named after Hollywood animation characters: Fluorescence Imaging with One Nanometer Accuracy (FIONA), Single-molecule High-REsolution Colocalization (SHREC), and Defocused Orientation and Position Imaging (DOPI). We explain new understanding of molecular motors obtained from measurements using these techniques.

Studying Molecular Motors on the Single Molecule Level

2003 ◽  
pp. 273-292 ◽  
Author(s):  
Y. Ishii ◽  
A. H. Iwane ◽  
H. Yokota ◽  
Y. Inoue ◽  
T. Wazawa ◽  
...  
Keyword(s):  
Single Molecule ◽  
Molecular Motors ◽  
Single Molecule Level

Atomic Force Microscopy (AFM) for Topography and Recognition Imaging at Single Molecule Level

2013 ◽  
pp. 102-112
Author(s):  
Memed Duman ◽  
Andreas Ebner ◽  
Christian Rankl ◽  
Jilin Tang ◽  
Lilia A. Chtcheglova ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Single Molecule ◽  
Single Molecule Level ◽  
Force Microscopy ◽  
Atomic Force ◽  
Recognition Imaging
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