Perinuclear theca during spermatozoa maturation leading to fertilization

2003 ◽  
Vol 61 (1) ◽  
pp. 76-87 ◽  
Author(s):  
Adela Müjica ◽  
Fernando Navarro-García ◽  
Enrique O. Hernández-González ◽  
María de Lourdes Juárez-Mosqueda
Keyword(s):  
Perinuclear Theca ◽  
Spermatozoa Maturation

Biogenesis of the sperm head perinuclear theca during human spermiogenesis

2009 ◽  
Vol 92 (4) ◽  
pp. 1472-1473 ◽  
Author(s):  
Cristian Alvarez Sedó ◽  
Richard Oko ◽  
Peter Sutovsky ◽  
Héctor Chemes ◽  
Vanesa Y. Rawe
Keyword(s):  
Sperm Head ◽  
Perinuclear Theca

Cryopreservation of bull spermatozoa alters the perinuclear theca

2006 ◽  
Vol 66 (8) ◽  
pp. 1969-1975 ◽  
Author(s):  
Carmen Omega Martínez ◽  
María de Lourdes Juárez-Mosqueda ◽  
Jorge Hernández ◽  
Javier Valencia
Keyword(s):  
Perinuclear Theca

scholarly journals Core Histones Are Constituents of the Perinuclear Theca of Murid Spermatozoa: An Assessment of Their Synthesis and Assembly during Spermiogenesis and Function after Gametic Fusion

2021 ◽  
Vol 22 (15) ◽  
pp. 8119
Author(s):  
Lauren E. Hamilton ◽  
Morgan Lion ◽  
Luis Aguila ◽  
João Suzuki ◽  
Genevieve Acteau ◽  
...  
Keyword(s):  
Chromatin Remodeling ◽  
De Novo ◽  
Sperm Nucleus ◽  
Sperm Head ◽  
Cell Fractionation ◽  
Cellular Processes ◽  
Functional Potential ◽  
Core Histones ◽  
Perinuclear Theca ◽  
And Function

The perinuclear theca (PT) of the eutherian sperm head is a cytoskeletal-like structure that houses proteins involved in important cellular processes during spermiogenesis and fertilization. Building upon our novel discovery of non-nuclear histones in the bovine PT, we sought to investigate whether this PT localization was a conserved feature of eutherian sperm. Employing cell fractionation, immunodetection, mass spectrometry, qPCR, and intracytoplasmic sperm injections (ICSI), we examined the localization, developmental origin, and functional potential of histones from the murid PT. Immunodetection localized histones to the post-acrosomal sheath (PAS) and the perforatorium (PERF) of the PT but showed an absence in the sperm nucleus. MS/MS analysis of selectively extracted PT histones indicated that predominately core histones (i.e., H3, H3.3, H2B, H2A, H2AX, and H4) populate the murid PT. These core histones appear to be de novo-synthesized in round spermatids and assembled via the manchette during spermatid elongation. Mouse ICSI results suggest that early embryonic development is delayed in the absence of PT-derived core histones. Here, we provide evidence that core histones are de novo-synthesized prior to PT assembly and deposited in PT sub-compartments for subsequent involvement in chromatin remodeling of the male pronucleus post-fertilization.

The mouse transcription factor Stat4 is expressed in haploid male germ cells and is present in the perinuclear theca of spermatozoa

1997 ◽  
Vol 110 (14) ◽  
pp. 1543-1553 ◽  
Author(s):  
G. Herrada ◽  
D.J. Wolgemuth
Keyword(s):  
Transcription Factor ◽  
Germ Cells ◽  
Nuclear Translocation ◽  
Biological Effects ◽  
Immunoblot Analysis ◽  
Male Germ Cells ◽  
Haploid Male ◽  
Zygotic Transcription ◽  
The Hours ◽  
Perinuclear Theca

STAT (signal transducer and activator of transcription) proteins have been shown to be essential transcription factors which mediate biological effects of cytokines. Although most of the STATs have been shown to be widely expressed, Stat4 mRNA has been detected in only a few tissues, including the testis. In the present study, immunoblot analysis confirmed that the presence of Stat4 protein was similarly restricted, with the highest level observed in testis. In situ hybridization, immunoblot, and immunohistochemistry analyses revealed that in the testis, Stat4 was abundantly and exclusively expressed in male germ cells which have completed meiosis, at the round and elongating spermatid stages. Cytolocalization at various times of spermatid differentiation showed that the level of Stat4 protein increased in parallel in both cytoplasm and nuclei. No specific nuclear translocation that would have been an indicator of Stat4 activation was observed at any stage of spermatogenic differentiation. Interestingly, the Stat4 transcription factor was localized to the condensing perinuclear theca of spermatids, a localization that was confirmed by selective biochemical extraction of thecal proteins. Since the theca is known to depolymerize in the cytoplasm of the oocyte during the hours following fertilization, we hypothesized that sperm Stat4 would represent an original paternal contribution to the fertilized egg which may be involved in the onset of zygotic transcription.

Glycerol decreases the integrity of the perinuclear theca in boar sperm

Zygote ◽  
2012 ◽  
Vol 21 (2) ◽  
pp. 172-177 ◽  
Author(s):  
Marco Antonio Arenas Núñez ◽  
María de Lourdes Juárez-Mosqueda ◽  
Oscar Gutiérrez-Pérez ◽  
Santiago René Anzaldúa Arce ◽  
Alejandro Córdova Izquierdo ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Adverse Effect ◽  
Protease Inhibitors ◽  
Free Group ◽  
Glycerol Concentration ◽  
Boar Sperm ◽  
Perinuclear Theca ◽  
First Time ◽  
Control Samples

SummaryWe evaluated the effect of glycerol on the perinuclear theca (PT) of boar sperm. Samples from six ejaculates obtained from three different boars were incubated in the detergent Brij 36-T. Spermatozoa were treated with a glycerol concentration of either 2 or 4%, and incubated for 10 or 30 min; two other samples were treated with protease inhibitors (PI; leupeptin or an inhibitor commercial cocktail), mixed with 4% glycerol, and incubated for 30 min. A third glycerol-free group was used as the control. The samples were processed for electron microscopy evaluation. The PT remained intact in 78% of the control samples while, after addition of glycerol for 30 min, the proportion of spermatozoa with disrupted or absent PT increased (P < 0.05). PT was preserved in PI samples, but PT changes increased (P < 0.05). Differences due to treatment with glycerol (2 or 4%) at 10 or 30 min were not observed. These results show, to our knowledge for the first time, the adverse effect of glycerol on the integrity of the PT.

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