Endothelial cell–Matrix interactions

2003 ◽  
Vol 60 (1) ◽  
pp. 13-22 ◽  
Author(s):  
Erika Iivanainen ◽  
Veli-Matti Kähäri ◽  
Jyrki Heino ◽  
Klaus Elenius
Keyword(s):  
Endothelial Cell ◽  
Cell Matrix ◽  
Matrix Interactions ◽  
Cell Matrix Interactions

Endothelial Cell–Matrix Interactions in Neovascularization

2008 ◽  
Vol 14 (1) ◽  
pp. 19-32 ◽  
Author(s):  
Megan E. Francis ◽  
Shiri Uriel ◽  
Eric M. Brey
Keyword(s):  
Endothelial Cell ◽  
Cell Matrix ◽  
Matrix Interactions ◽  
Cell Matrix Interactions

scholarly journals Induction of Caspase-Mediated Cell Death by Matrix Metalloproteinases in Cerebral Endothelial Cells after Hypoxia—Reoxygenation

2004 ◽  
Vol 24 (7) ◽  
pp. 720-727 ◽  
Author(s):  
Sun-Ryung Lee ◽  
Eng H. Lo
Keyword(s):  
Endothelial Cells ◽  
Cell Death ◽  
Endothelial Cell ◽  
Matrix Metalloproteinases ◽  
Caspase 3 ◽  
Cell Matrix ◽  
Matrix Interactions ◽  
Endothelial Cell Death ◽  
Cell Matrix Interactions ◽  
Hypoxia Reoxygenation

Matrix metalloproteinases (MMPs) may contribute to the pathophysiology of cerebral ischemia by degrading matrix components in the neurovascular unit. In this study, the authors document a pathway by which MMPs interfere with cell—matrix interactions and trigger caspase-mediated cytotoxicity in brain endothelial cells. Hypoxia—reoxygenation induced endothelial cytotoxicity. Cytoprotection with zDEVD-fmk confirmed that cell death was partly caspase mediated. The temporal profile of caspase-3 activation was matched by elevations in MMP-2 and MMP-9. MMP inhibitors significantly decreased caspase-3 activation and reduced endothelial cell death. Degradation of matrix fibronectin confirmed the presence of extracellular proteolysis. Increasing integrin-linked kinase signaling with the β1 integrin-activating antibody (8A2) ameliorated endothelial cytotoxicity. The results suggest that MMP-9 and MMP-2 contribute to caspase-mediated brain endothelial cell death after hypoxia—reoxygenation by disrupting cell—matrix interactions and homeostatic integrin signaling.

scholarly journals PECAM-1 regulates proangiogenic properties of endothelial cells through modulation of cell-cell and cell-matrix interactions

2010 ◽  
Vol 299 (6) ◽  
pp. C1468-C1484 ◽  
Author(s):  
SunYoung Park ◽  
Terri A. DiMaio ◽  
Elizabeth A. Scheef ◽  
Christine M. Sorenson ◽  
Nader Sheibani
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Cell Adhesion ◽  
Endothelial Cell ◽  
Immunoglobulin Superfamily ◽  
Capillary Morphogenesis ◽  
Cell Matrix ◽  
Matrix Interactions ◽  
Cell Matrix Interactions ◽  
Cell Cell

Platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31) is a member of the immunoglobulin superfamily of cell adhesion molecules with important roles in angiogenesis and inflammation. However, the molecular and cellular mechanisms, and the role that specific PECAM-1 isoforms play in these processes, remain elusive. We recently showed attenuation of retinal vascular development and neovascularization in PECAM-1-deficient (PECAM-1−/−) mice. To gain further insight into the role of PECAM-1 in these processes, we isolated primary retinal endothelial cells (EC) from wild-type (PECAM-1+/+) and PECAM-1−/− mice. Lack of PECAM-1 had a significant impact on endothelial cell-cell and cell-matrix interactions, resulting in attenuation of cell migration and capillary morphogenesis. Mechanistically these changes were associated with a significant decrease in expression of endothelial nitric oxide synthase (eNOS) and nitric oxide (NO) bioavailability in PECAM-1−/− retinal EC. PECAM-1−/− retinal EC also exhibited a lower rate of apoptosis under basal and challenged conditions, consistent with their increased growth rate. Furthermore, reexpression of PECAM-1 was sufficient to restore migration and capillary morphogenesis of null cells in an isoform-specific manner. Thus PECAM-1 expression modulates proangiogenic properties of EC, and these activities are significantly influenced by alternative splicing of its cytoplasmic domain.

scholarly journals Endothelial cell-matrix interactions determine maturation of dendritic cells

2007 ◽  
Vol 37 (7) ◽  
pp. 1773-1784 ◽  
Author(s):  
Heiko Methe ◽  
Shmuel Hess ◽  
Elazer R. Edelman
Keyword(s):  
Dendritic Cells ◽  
Endothelial Cell ◽  
Cell Matrix ◽  
Matrix Interactions ◽  
Cell Matrix Interactions

Endothelial Cell–Matrix Interactions in Neovascularization

2008 ◽  
pp. 110306233438005
Author(s):  
Megan E. Francis ◽  
Shiri Uriel ◽  
Eric M. Brey
Keyword(s):  
Endothelial Cell ◽  
Cell Matrix ◽  
Matrix Interactions ◽  
Cell Matrix Interactions

Endothelial Cell-Matrix Interactions in Health and Disease

1991 ◽  
pp. 197-202
Author(s):  
Elisabetta Dejana ◽  
A. Zanetti ◽  
C. Dominguez-Jimenez ◽  
G. Conforti
Keyword(s):  
Endothelial Cell ◽  
Cell Matrix ◽  
Matrix Interactions ◽  
Health And Disease ◽  
Cell Matrix Interactions
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