Butanol production by Clostridium acetobutylicum ATCC 824 by electro‐fermentation in culture medium supplemented with butyrate and neutral red

2021 ◽  
Author(s):  
Karlo Guerrero ◽  
Roberto Gallardo ◽  
Ernesto González ◽  
Fabián Veliz ◽  
Raúl Conejeros ◽  
...  
Keyword(s):  
Clostridium Acetobutylicum ◽  
Culture Medium ◽  
Neutral Red ◽  
Butanol Production ◽  
Clostridium Acetobutylicum Atcc 824

scholarly journals Expression of abrB310 and sinR, and Effects of Decreased abrB310 Expression on the Transition from Acidogenesis to Solventogenesis, in Clostridium acetobutylicum ATCC 824

2005 ◽  
Vol 71 (4) ◽  
pp. 1987-1995 ◽  
Author(s):  
Miles C. Scotcher ◽  
Frederick B. Rudolph ◽  
George N. Bennett
Keyword(s):  
Antisense Rna ◽  
Clostridium Acetobutylicum ◽  
Growth Conditions ◽  
Butanol Production ◽  
Transcription Start ◽  
Transcription Start Sites ◽  
Consensus Motifs ◽  
Sporulation Initiation ◽  
Transient Elevation ◽  
Clostridium Acetobutylicum Atcc 824

ABSTRACT The transcription factors sinR and abrB are involved in the control of sporulation initiation in Bacillus subtilis. We identified a single homologue to sinR and three highly similar homologues to abrB, designated abrB310, abrB1941, and abrB3647, in Clostridium acetobutylicum ATCC 824. Using reporter vectors, we showed that the promoters of abrB1941 and abrB3647 were not active under the growth conditions tested. The abrB310 promoter was strongly active throughout growth and exhibited a transient elevation of expression at the onset of solventogenesis. Primer extension assays showed that two transcripts of abrB310 and a single, extremely weak transcript for sinR are expressed. Potential −35 and −10 consensus motifs are readily identifiable surrounding the transcription start sites of abrB310 and sinR, with a single putative 0A box present within the promoter of abrB310. In strains of C. acetobutylicum transformed with plasmids to elevate sinR expression or decrease sinR expression, no significant differences in growth or in acid or solvent production were observed compared to the control strains. In C. acetobutylicum strain 824(pAS310), which expressed an antisense RNA construct targeted against abrB310, the acids acetate and butyrate accumulated to approximately twice the normal concentration. This accumulation corresponded to a delay and decrease in acetone and butanol production. It was also found that sporulation in strain 824(pAS310) was delayed but that the morphology of sporulating cells and spores was normal. Based upon these observations, we propose that abrB310 may act as a regulator at the transition between acidogenic and solventogenic growth.

scholarly journals The significance of aspartate on NAD(H) biosynthesis and ABE fermentation in Clostridium acetobutylicum ATCC 824

AMB Express ◽  
2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Zhengping Liao ◽  
Xitong Yang ◽  
Hongxin Fu ◽  
Jufang Wang
Keyword(s):  
Clostridium Acetobutylicum ◽  
Wild Type Strain ◽  
De Novo ◽  
Reference Value ◽  
Butanol Production ◽  
Wild Type ◽  
De Novo Synthesis ◽  
Fermentation Performance ◽  
Engineering Strategy ◽  
Clostridium Acetobutylicum Atcc 824

Abstract The co-factor NADH plays an important role in butanol biosynthesis. In this study, we found that aspartate could effectively improve the butanol production of Clostridium acetobutylicum ATCC 824. Further study showed that aspartate could be used as the precursor of NADH de novo synthesis in C. acetobutylicum ATCC 824. When 2 g/L aspartate was added, the transcription levels of essential genes (nadA, nadB and nadC) for NADH de novo synthesis were significantly higher than that of without aspartate addition. The levels of intracellular NAD+, NADH, total NAD(H) and the ratio of NADH/NAD+ were also significantly increased, which were 63.9 ± 8.0%, 85.0 ± %, 77.7 ± 8.0% and 12.7 ± 2.9% higher than those of without aspartate addition, respectively. Furthermore, the butanol production was improved by overexpressing the NADH de novo synthesis genes, and the fermentation performance could be further enhanced by strengthening the VB1 biosynthesis and NADH de novo synthesis pathway simultaneously. As a result, the butanol titer of the engineered strain 824(thiCGE–nadC) reached 13.96 ± 0.11 g/L, 7.2 ± 0.4%, 18.1 ± 0.1%, 34.1 ± 0.1% higher than that of 824(thiCGE), 824(nadC) and the wild type strain, respectively. This study has a reference value for the NADH related researches of other microbes, and the engineering strategy used in this study provides a new idea for construction of efficient fuel-producing strains.

Expression of Cloned Homologous Fermentative Genes in Clostridium Acetobutylicum ATCC 824

1992 ◽  
Vol 10 (2) ◽  
pp. 190-195 ◽  
Author(s):  
Lee D. Mermelstein ◽  
Neil E. Welker ◽  
George N. Bennett ◽  
Eleftherios T. Papoutsakis
Keyword(s):  
Clostridium Acetobutylicum ◽  
Clostridium Acetobutylicum Atcc 824

Two stage biodiesel and hydrogen production from molasses by oleaginous fungi and Clostridium acetobutylicum ATCC 824

2014 ◽  
Vol 39 (7) ◽  
pp. 3185-3197 ◽  
Author(s):  
Magdy Mohamed Khalil Bagy ◽  
Mohamed Hemida Abd-Alla ◽  
Fatthy Mohamed Morsy ◽  
Elhagag Ahmed Hassan
Keyword(s):  
Hydrogen Production ◽  
Clostridium Acetobutylicum ◽  
Two Stage ◽  
Oleaginous Fungi ◽  
Clostridium Acetobutylicum Atcc 824

Integrated ABE fermentation–adsorption process for enhanced butanol production by Clostridium acetobutylicum

2018 ◽  
Vol 44 ◽  
pp. S41
Author(s):  
F. Raganati ◽  
A. Procentese ◽  
G. Olivieri ◽  
M.E. Russo ◽  
P. Salatino ◽  
...  
Keyword(s):  
Clostridium Acetobutylicum ◽  
Adsorption Process ◽  
Abe Fermentation ◽  
Butanol Production
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