Contribution of protein A step towards cost of goods for continuous production of monoclonal antibody therapeutics

2021 ◽  
Author(s):  
Vikrant Bansode ◽  
Paridhi Gupta ◽  
Nikhil Kateja ◽  
Anurag S Rathore
Keyword(s):  
Monoclonal Antibody ◽  
Continuous Production ◽  
Protein A ◽  
Antibody Therapeutics

Non-protein A purification platform for continuous processing of monoclonal antibody therapeutics

2018 ◽  
Vol 1579 ◽  
pp. 60-72 ◽  
Author(s):  
Nikhil Kateja ◽  
Devashish Kumar ◽  
Suruchi Sethi ◽  
Anurag S. Rathore
Keyword(s):  
Monoclonal Antibody ◽  
Protein A ◽  
Continuous Processing ◽  
Antibody Therapeutics

Assay for islet cell antibodies. Protein A--monoclonal antibody method

Diabetes ◽  
1985 ◽  
Vol 34 (3) ◽  
pp. 300-305 ◽  
Author(s):  
S. Srikanta ◽  
A. Rabizadeh ◽  
M. A. Omar ◽  
G. S. Eisenbarth
Keyword(s):  
Monoclonal Antibody ◽  
Islet Cell ◽  
Protein A ◽  
Islet Cell Antibodies ◽  
Antibody Method

Evaluation of the Efficiency of Protein A Affinity Chromatography to Purify a Monoclonal Antibody for Cancer Treatment and its Purity Analysis

2020 ◽  
Vol 7 (2) ◽  
pp. 121-133
Author(s):  
Ayesha Akhtar ◽  
Shivakumar Arumugam ◽  
Shoaib Alam
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Affinity Chromatography ◽  
Protein A ◽  
Exchange Chromatography ◽  
Size Exclusion ◽  
High Yield ◽  
Staphylococcal Protein A ◽  
Purification Step ◽  
Purity Analysis

Background:: Protein A affinity chromatography is often employed as the most crucial purification step for monoclonal antibodies to achieve high yield with purity and throughput requirements. Introduction:: Protein A, also known as Staphylococcal protein A (SPA) is found in the cell wall of the bacteria staphylococcus aureus. It is one of the first discovered immunoglobulin binding molecules and has been extensively studied since the past few decades. The efficiency of Protein A affinity chromatography to purify a recombinant monoclonal antibody in a cell culture sample has been evaluated, which removes 99.0% of feed stream impurities. Materials and Method:: We have systematically evaluated the purification performance by using a battery of analytical methods SDS-PAGE (non-reduced and reduced sample), Cation Exchange Chromatography (CEX), Size-exclusion chromatography (SEC), and Reversed phased-Reduced Chromatography for a CHO-derived monoclonal antibody. Results and Discussion:: The analytical test was conducted to determine the impurity parameter, Host Cell Contaminating Proteins (HCP). It was evaluated to be 0.015ng/ml after the purification step; while initially, it was found to be 24.431ng/ml. Conclusion:: The tests showed a distinct decrease in the level of different impurities after the chromatography step. It can be concluded that Protein A chromatography is an efficient step in the purification of monoclonal antibodies.

scholarly journals Single Chain Variable Fragment Fused to Maltose Binding Protein: A Modular Nanocarrier Platform for the Targeted Delivery of Antitumorals

2021 ◽  
Author(s):  
Francisco J. Reche-Perez ◽  
Simona Plesselova ◽  
Eduardo De los Reyes-Berbel ◽  
Mariano Ortega-Muñoz ◽  
F. Javier Lopez-Jaramillo ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Targeted Delivery ◽  
Specific Binding ◽  
Protein A ◽  
Antibody Fragments ◽  
Single Chain Variable Fragment ◽  
Single Chain ◽  
Binding Properties ◽  
Single Chain Variable Fragments ◽  
Selective Delivery

The use of the specific binding properties of monoclonal antibody fragments such as single-chain variable fragments (ScFv) for the selective delivery of antitumor therapeutics for cancer cells is attractive due...

scholarly journals Immunoelectron microscopic localization of an oviductal antigen in hamster zona pellucida by use of a monoclonal antibody.

1988 ◽  
Vol 36 (11) ◽  
pp. 1441-1447 ◽  
Author(s):  
F W Kan ◽  
S St-Jacques ◽  
G Bleau
Keyword(s):  
Monoclonal Antibody ◽  
Zona Pellucida ◽  
Preimplantation Embryo ◽  
Protein A ◽  
Cumulus Cells ◽  
Gold Particles ◽  
Antigenic Sites ◽  
Cumulus Oophorus ◽  
Entire Thickness ◽  
Immunocytochemical Method

The zona pellucida is an extracellular matrix of glycoproteins which surrounds the mammalian oocyte and preimplantation embryo. We have recently developed monoclonal antibodies against oviductal zona pellucida of the golden hamster. We applied the post-embedding immunocytochemical method using a monoclonal antibody (IgGl,k) to determine the precise location of antigenic sites in the cumulus oophorus complex of the superovulated hamster. By applying the high-resolution protein A-gold technique, we demonstrated that the sites of immunoreactivity were exclusively in the zona pellucida encompassing the oocyte. Other structures within the oocyte and neighboring cumulus cells were not labeled by gold particles. Moreover, gold particles were evenly distributed throughout the entire thickness of the zona pellucida, indicating that this extracellular layer is at least in part made up of an antigen recognized by the monoclonal antibody that is uniformly distributed in the zona matrix.

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