Efficient use of brewer's spent grain hydrolysates in ABE fermentation by Clostridium beijerinkii . Effect of high solid loads in the enzymatic hydrolysis

2020 ◽  
Vol 95 (9) ◽  
pp. 2393-2402
Author(s):  
Pedro E Plaza ◽  
Mónica Coca ◽  
Susana Lucas ◽  
Marina Fernández-Delgado ◽  
Juan C López-Linares ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Abe Fermentation ◽  
Brewer’S Spent Grain ◽  
High Solid ◽  
Spent Grain

scholarly journals Intensifying ethanol production from brewer’s spent grain waste: Use of whole slurry at high solid loadings

2019 ◽  
Vol 53 ◽  
pp. 1-8 ◽  
Author(s):  
Tânia Pinheiro ◽  
Eduardo Coelho ◽  
Aloia Romaní ◽  
Lucília Domingues
Keyword(s):  
Ethanol Production ◽  
Brewer’S Spent Grain ◽  
High Solid ◽  
Spent Grain ◽  
Whole Slurry

Effect of a milling pre-treatment on the enzymatic hydrolysis of carbohydrates in brewer’s spent grain

2012 ◽  
Vol 116 ◽  
pp. 155-160 ◽  
Author(s):  
Piritta Niemi ◽  
Craig B. Faulds ◽  
Juhani Sibakov ◽  
Ulla Holopainen ◽  
Kaisa Poutanen ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Brewer’S Spent Grain ◽  
Spent Grain ◽  
Pre Treatment ◽  
Hydrolysis Of

scholarly journals Optimised Fractionation of Brewer’s Spent Grain for a Biorefinery Producing Sugars, Oligosaccharides, and Bioethanol

Processes ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 366
Author(s):  
Soma Bedő ◽  
Margaréta Rozbach ◽  
Leonóra Nagy ◽  
Anikó Fehér ◽  
Csaba Fehér
Keyword(s):  
Enzymatic Hydrolysis ◽  
Ethanol Yield ◽  
Desirability Function ◽  
Value Added ◽  
Fractionation Process ◽  
Acidic Hydrolysis ◽  
Brewer’S Spent Grain ◽  
Platform Chemicals ◽  
Brewing Process ◽  
Spent Grain

Brewer’s spent grain (BSG) is the main by-product of the beer brewing process. It has a huge potential as a feedstock for bio-based manufacturing processes to produce high-value bio-products, biofuels, and platform chemicals. For the valorisation of BSG in a biorefinery process, efficient fractionation and bio-conversion processes are required. The aim of our study was to develop a novel fractionation of BSG for the production of arabinose, arabino-xylooligomers, xylose, and bioethanol. A fractionation process including two-step acidic and enzymatic hydrolysis steps was investigated and optimised by a response surface methodology and a desirability function approach to fractionate the carbohydrate content of BSG. In the first acidic hydrolysis, high arabinose yield (76%) was achieved under the optimised conditions (90 °C, 1.85 w/w% sulphuric acid, 19.5 min) and an arabinose- and arabino-xylooligomer-rich supernatant was obtained. In the second acidic hydrolysis, the remaining xylan was solubilised (90% xylose yield) resulting in a xylose-rich hydrolysate. The last, enzymatic hydrolysis step resulted in a glucose-rich supernatant (46 g/L) under optimised conditions (15 w/w% solids loading, 0.04 g/g enzyme dosage). The glucose-rich fraction was successfully used for bioethanol production (72% ethanol yield by commercial baker’s yeast). The developed and optimised process offers an efficient way for the value-added utilisation of BSG. Based on the validated models, the amounts of the produced sugars, the composition of the sugar streams and solubilised oligo-saccharides are predictable and variable by changing the reaction conditions of the process.

scholarly journals EFFECT OF REACTION TIME AND SODIUM HYDROXIDE CONCENTRATION ON DELIGNIFICATION AND ENZYMATIC HYDROLYSISOF BREWER’S SPENT GRAIN FROM WO BRAZILIAN BREWERS

2021 ◽  
Vol 55 (1-2) ◽  
pp. 101-112
Author(s):  
CARLA LOBO GOMES ◽  
ELIZABETH GONÇALVES ◽  
CARLOS ALBERTO GALEANO SUAREZ ◽  
DASCIANA DE SOUSA RODRIGUES ◽  
INTI CAVALCANTI MONTANO
Keyword(s):  
Reaction Time ◽  
Enzymatic Hydrolysis ◽  
Factorial Design ◽  
Contact Time ◽  
Point Contact ◽  
Sodium Hydroxide Concentration ◽  
Brewer’S Spent Grain ◽  
Spent Grain ◽  
Hydrolysis Of

Brazil, being one of the main beer producers, generates brewer’s spent grain as a main by-product of this industry, which is mainly composed of cellulose, hemicelluloses, lignin and extractives. The alkaline pretreatment of brewer’s spent grain received from two breweries is studied in this work, namely breweries Imperial (B1) and Colombina (B2). Factorial design (22) was realized with three experiments at the central point: contact time (30, 60, 90 min) and NaOH concentration (4%, 6%, 8%). It was found that the presence of extractives causes interference in the characterization of the material. The delignification process allowed obtaining materials with lower lignin percentages when higher NaOH concentrations were used, reaching percentages of lignin loss with values between 85-95%, in both materials, but for these conditions, the losses of cellulose were considerable – of 35-43%. Enzymatic hydrolysis of the pretreated materials achieved conversions greater than 70%, emphasizing that the greatest conversions were obtained with material B1, where almost the whole cellulose was hydrolyzed.

scholarly journals Process simulation for xylitol production from brewer’s spent grain in a Colombian biorefinery. Part 1: Xylose production from arabinoxilans extracted by the alkaline pretreatment of BSG

2019 ◽  
Vol 39 (1) ◽  
Author(s):  
Andrés Alfonso Gil Montenegro ◽  
Juan Sebastian Arocha Morales ◽  
Lilia Carolina Rojas Pérez ◽  
Paulo César Narváez Rincón
Keyword(s):  
Enzymatic Hydrolysis ◽  
Dry Weight ◽  
Alkaline Pretreatment ◽  
Raw Material ◽  
Brewing Industry ◽  
Brewer’S Spent Grain ◽  
Spent Grain ◽  
Two Stages ◽  
Xylose Production ◽  
Hydrolysis Of

This work presents the simulation in Aspen Plusr of a process to obtain arabinoxylans (AX) from Brewer’s Spent Grain (BSG), which is the major byproduct of the brewing industry. The process is divided into two stages: alkaline pretreatment and enzymatic hydrolysis. These stages cover the extraction of proteins and AX from BSG using an alkaline pretreatment and enzymatic hydrolysis of the AX separated from the liquid stream to obtain xylose, i.e. the substrate required for the fermentation to xylitol. Simulation results show that xylose obtained corresponds to 8,5% of the dry weight of the raw material, obtaining a yield of 58%. Several streams of byproducts were obtained, such as proteins, polypeptides, amino acids, phenolic compounds and lignocellulosic residues that can be valorized in other processes. Simulation was performed in the context of a biorefinery in Colombia.

Ultrasound Technology Applied to Enhance Enzymatic Hydrolysis of Brewer’s Spent Grain and its Potential for Production of Fermentable Sugars

2018 ◽  
Vol 10 (8) ◽  
pp. 2157-2164 ◽  
Author(s):  
Luciana Luft ◽  
Tássia C. Confortin ◽  
Izelmar Todero ◽  
Juliana R. F. da Silva ◽  
Laura P. Tovar ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Fermentable Sugars ◽  
Brewer’S Spent Grain ◽  
Spent Grain ◽  
Ultrasound Technology ◽  
Hydrolysis Of
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