Isobutyrate biosynthesis via methanol chain elongation: converting organic wastes to platform chemicals

2016 ◽  
Vol 92 (6) ◽  
pp. 1370-1379 ◽  
Author(s):  
Wei-Shan Chen ◽  
Shengle Huang ◽  
David PBTB Strik ◽  
Cees JN Buisman
Keyword(s):  
Organic Wastes ◽  
Chain Elongation ◽  
Platform Chemicals

scholarly journals Three Novel Clostridia Isolates Produce n-Caproate and iso-Butyrate from Lactate: Comparative Genomics of Chain-Elongating Bacteria

2020 ◽  
Vol 8 (12) ◽  
pp. 1970
Author(s):  
Bin Liu ◽  
Denny Popp ◽  
Nicolai Müller ◽  
Heike Sträuber ◽  
Hauke Harms ◽  
...  
Keyword(s):  
Comparative Genomics ◽  
Energy Conservation ◽  
Core Genome ◽  
Anaerobic Fermentation ◽  
Batch Cultivation ◽  
Chain Elongation ◽  
Hydrogen Formation ◽  
Lactate Oxidation ◽  
Platform Chemicals ◽  
Genome Heterogeneity

The platform chemicals n-caproate and iso-butyrate can be produced by anaerobic fermentation from agro-industrial residues in a process known as microbial chain elongation. Few lactate-consuming chain-elongating species have been isolated and knowledge on their shared genetic features is still limited. Recently we isolated three novel clostridial strains (BL-3, BL-4, and BL-6) that convert lactate to n-caproate and iso-butyrate. Here, we analyzed the genetic background of lactate-based chain elongation in these isolates and other chain-elongating species by comparative genomics. The three strains produced n-caproate, n-butyrate, iso-butyrate, and acetate from lactate, with the highest proportions of n-caproate (18%) for BL-6 and of iso-butyrate (23%) for BL-4 in batch cultivation at pH 5.5. They show high genomic heterogeneity and a relatively small core-genome size. The genomes contain highly conserved genes involved in lactate oxidation, reverse β-oxidation, hydrogen formation and either of two types of energy conservation systems (Rnf and Ech). Including genomes of another eleven experimentally validated chain-elongating strains, we found that the chain elongation-specific core-genome encodes the pathways for reverse β-oxidation, hydrogen formation and energy conservation, while displaying substantial genome heterogeneity. Metabolic features of these isolates are important for biotechnological applications in n-caproate and iso-butyrate production.

scholarly journals Highly selective recovery of medium chain carboxylates from co-fermented organic wastes using anion exchange with CO2-expanded methanol desorption

2020 ◽  
Author(s):  
Clara Fernando-Foncillas
Keyword(s):  
Anion Exchange ◽  
Organic Wastes ◽  
Chain Elongation ◽  
Selective Recovery ◽  
Medium Chain ◽  
The Right

Contribution to the International Chain Elongation Conference 2020 | ICEC 2020. An abstract can be found in the right column.

Stable production of platform chemicals from the continuous hydrodeoxygenation of a raw bio-oil using enhanced carbon-based catalysts

2020 ◽  
Author(s):  
Idoia Hita ◽  
Tomas Cordero-Lanzac ◽  
Francisco J. Garcia-Mateos ◽  
Jose Rodriguez-Mirasol ◽  
Tomas Cordero ◽  
...  
Keyword(s):  
Platform Chemicals ◽  
Stable Production ◽  
Bio Oil ◽  
Carbon Based

Stable production of platform chemicals from the continuous hydrodeoxygenation of a raw bio-oil using enhanced carbon-based catalysts

2020 ◽  
Author(s):  
Idoia Hita ◽  
Tomas Cordero-Lanzac ◽  
Francisco J. Garcia-Mateos ◽  
Jose Rodriguez-Mirasol ◽  
Tomas Cordero ◽  
...  
Keyword(s):  
Platform Chemicals ◽  
Stable Production ◽  
Bio Oil ◽  
Carbon Based

Unravelling a microbial synergy to boost caproate production via carboxylates chain elongation with ethanol

2019 ◽  
Vol 26 (2) ◽  
pp. 63-71
Author(s):  
Ling Leng ◽  
Ying Wang ◽  
Peixian Yang ◽  
Takashi Narihiro ◽  
Masaru Konishi Nobu ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
Microbial Consortia ◽  
Chain Elongation ◽  
Desulfovibrio Vulgaris ◽  
Rrna Gene ◽  
Selective Enrichment ◽  
Microbial Network ◽  
Cost Efficient ◽  
Rrna Gene Sequencing

Chain elongation of volatile fatty acids for medium chain fatty acids production (e.g. caproate) is an attractive approach to treat wastewater anaerobically and recover resource simultaneously. Undefined microbial consortia can be tailored to achieve chain elongation process with selective enrichment from anaerobic digestion sludge, which has advantages over pure culture approach for cost-efficient application. Whilst the metabolic pathway of the dominant caproate producer, Clostridium kluyveri, has been annotated, the role of other coexisting abundant microbiomes remained unclear. To this end, an ethanol-acetate fermentation inoculated with fresh digestion sludge at optimal conditions was conducted. Also, physiological study, thermodynamics and 16 S rRNA gene sequencing to elucidate the biological process by linking the system performance and dominant microbiomes were integrated. Results revealed a possible synergistic network in which C. kluyveri and three co-dominant species, Desulfovibrio vulgaris, Fusobacterium varium and Acetoanaerobium sticklandii coexisted. D. vulgaris and A. sticklandii (F. varium) were likely to boost the carboxylates chain elongation by stimulating ethanol oxidation and butyrate production through a syntrophic partnership with hydrogen (H2) serving as an electron messenger. This study unveils a synergistic microbial network to boost caproate production in mixed culture carboxylates chain elongation.

Impacts of Organic Wastes on Plankton Population of Woji Creek, Port Harcourt, Rivers State, Nigeria

2018 ◽  
Vol 5 (01-02 & Sp1) ◽  
Author(s):  
Utibe I. Daniel ◽  
◽  
Christiana C. Duru ◽  
Obemeata Oriakpono ◽  
M.O.E. Iwuala
Keyword(s):  
Organic Wastes ◽  
Port Harcourt ◽  
Rivers State ◽  
Plankton Population
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