Coupled ARTP and ALE strategy to improve anaerobic cell growth and succinic acid production byEscherichia coli

2015 ◽  
Vol 91 (3) ◽  
pp. 711-717 ◽  
Author(s):  
Jiang-feng Ma ◽  
Ming-ke Wu ◽  
Chang-qing Zhang ◽  
Ai-yong He ◽  
Xiang-ping Kong ◽  
...  
Keyword(s):  
Cell Growth ◽  
Succinic Acid ◽  
Acid Production ◽  
Succinic Acid Production ◽  
Byescherichia Coli

Promising advancement in fermentative succinic acid production by yeast hosts

2021 ◽  
Vol 401 ◽  
pp. 123414 ◽  
Author(s):  
Chong Li ◽  
Khai Lun Ong ◽  
Zhiyong Cui ◽  
Zhenyu Sang ◽  
Xiaotong Li ◽  
...  
Keyword(s):  
Succinic Acid ◽  
Acid Production ◽  
Succinic Acid Production

scholarly journals Metabolic Engineering of Escherichia coli for Enhanced Production of Succinic Acid, Based on Genome Comparison and In Silico Gene Knockout Simulation

2005 ◽  
Vol 71 (12) ◽  
pp. 7880-7887 ◽  
Author(s):  
Sang Jun Lee ◽  
Dong-Yup Lee ◽  
Tae Yong Kim ◽  
Byung Hun Kim ◽  
Jinwon Lee ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Succinic Acid ◽  
In Silico ◽  
Acid Production ◽  
Gene Knockout ◽  
Fermentation Products ◽  
E Coli ◽  
Metabolic Analysis ◽  
Enhanced Production ◽  
Succinic Acid Production

ABSTRACT Comparative analysis of the genomes of mixed-acid-fermenting Escherichia coli and succinic acid-overproducing Mannheimia succiniciproducens was carried out to identify candidate genes to be manipulated for overproducing succinic acid in E. coli. This resulted in the identification of five genes or operons, including ptsG, pykF, sdhA, mqo, and aceBA, which may drive metabolic fluxes away from succinic acid formation in the central metabolic pathway of E. coli. However, combinatorial disruption of these rationally selected genes did not allow enhanced succinic acid production in E. coli. Therefore, in silico metabolic analysis based on linear programming was carried out to evaluate the correlation between the maximum biomass and succinic acid production for various combinatorial knockout strains. This in silico analysis predicted that disrupting the genes for three pyruvate forming enzymes, ptsG, pykF, and pykA, allows enhanced succinic acid production. Indeed, this triple mutation increased the succinic acid production by more than sevenfold and the ratio of succinic acid to fermentation products by ninefold. It could be concluded that reducing the metabolic flux to pyruvate is crucial to achieve efficient succinic acid production in E. coli. These results suggest that the comparative genome analysis combined with in silico metabolic analysis can be an efficient way of developing strategies for strain improvement.

Optimization of fermentation medium for succinic acid production using Basfia succiniciproducens

2021 ◽  
pp. 101914
Author(s):  
Eleni Stylianou ◽  
Chrysanthi Pateraki ◽  
Dimitrios Ladakis ◽  
Anestis Vlysidis ◽  
Apostolis Koutinas
Keyword(s):  
Succinic Acid ◽  
Acid Production ◽  
Fermentation Medium ◽  
Succinic Acid Production ◽  
Optimization Of Fermentation

Opportunities, challenges, and future perspectives of succinic acid production by Actinobacillus succinogenes

2018 ◽  
Vol 102 (23) ◽  
pp. 9893-9910 ◽  
Author(s):  
Wubliker Dessie ◽  
Fengxue Xin ◽  
Wenming Zhang ◽  
Youming Jiang ◽  
Hao Wu ◽  
...  
Keyword(s):  
Succinic Acid ◽  
Acid Production ◽  
Actinobacillus Succinogenes ◽  
Future Perspectives ◽  
Succinic Acid Production
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