Effect of different fermentation parameters on bioethanol production from corn meal hydrolyzates by free and immobilized cells ofSaccharomyces cerevisiaevar.ellipsoideus

2009 ◽  
Vol 84 (4) ◽  
pp. 497-503 ◽  
Author(s):  
Svetlana Nikolić ◽  
Ljiljana Mojović ◽  
Marica Rakin ◽  
Dušanka Pejin ◽  
Viktor Nedović
Keyword(s):  
Immobilized Cells ◽  
Bioethanol Production ◽  
Corn Meal ◽  
Fermentation Parameters

Bioethanol Production by Pichia stipitis Immobilized on Water Hyacinth and Thin-Shell Silk Cocoon

2021 ◽  
Author(s):  
Suchata Kirdponpattara ◽  
Santi Chuetor ◽  
Malinee Sriariyanun ◽  
Muenduen Phisalaphong
Keyword(s):  
Water Hyacinth ◽  
Thin Shell ◽  
Immobilized Cells ◽  
High Surface ◽  
Pichia Stipitis ◽  
High Porosity ◽  
Bioethanol Production ◽  
Immobilized Cell ◽  
Silk Cocoon ◽  
Cell Carriers

Cell immobilization technique was applied in this study in order to examine effect of immobilized Pichia stipitis TISTR5806 on bioethanol production. Water hyacinth (WH) and thin-shell silk cocoon (CC) were used as cell carriers. Characteristics of the cell carriers were examined to explain the mechanism of bioethanol production. Carrier sizes and weights were optimized to improve bioethanol production. Moreover, stabilities of immobilized cells and carriers were evaluated. Because of high porosity, high surface area and good swelling ability of WH, cell immobilized on 1 g WH with 1 cm length produced the highest ethanol concentration at 13.3 g/L. Five cycles of a repeated batch of immobilized cell (IC) system on WH showed stable performance in ethanol production (8.2–10.4 g/L) with large numbers of the immobilized cells. The interaction between the immobilized cells and the WH surface were discovered.

scholarly journals Selenium supplementation during fermentation with sugar beet molasses and Saccharomyces cerevisiae to increase bioethanol production

2019 ◽  
Vol 8 (1) ◽  
pp. 622-628 ◽  
Author(s):  
Sara Faramarzi ◽  
Younes Anzabi ◽  
Hoda Jafarizadeh-Malmiri
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Sugar Beet ◽  
Sodium Selenite ◽  
Fermentation Process ◽  
Selenium Supplementation ◽  
Bioethanol Production ◽  
Beet Molasses ◽  
Sugar Beet Molasses ◽  
Fermentation Parameters ◽  
Microbial Strain

Abstract A bench scale submerged fermentation process was used to bioethanol produce using sugar beet molasses and Saccharomyces cerevisiae, as substrate and microbial strain, respectively. Effects of selenium amount on growth of S. cerevisiae and bioethanol production were evaluated. The obtained results indicated that growth of S. cerevisiae (manifested as turbidity intensity) in the samples containing 0, 5, 10, 15, 20 and 25 μg sodium selenite, during aerobic process, was 0.1707, 0.1678, 0.1679, 0.1664, 0.1627 and 0.160% a.u./h (after 14 h incubation), respectively. Statistical analysis based on compression test indicated that there were insignificant (p > 0.05) differences between growth rate of the yeast in the fermented samples containing S. cerevisiae and 5 to 25 μg selenium salt. Response surface methodology was utilized to evaluate effects of two fermentation parameters namely, amount of selenium (5-25 μg) and substrate brix (10-25°Bx) on the concentration (g/L) of produced bioethanol. Obtained results revealed that maximum bioethanol concentration (55 g/L) was achieved using 15 μg selenium and molasses with 25°Bx. Furthermore, results have also indicated that, without using selenium and using molasses with 25°Bx, bioethanol with concentration of 29 g/L was produced.

scholarly journals IMPROVEMENT OF BIOETHANOL PRODUCTION BY USING Saccharomyces cerevisiae [Meyen ex E.C. Hansen] IMMOBILIZED ON PRETREATED SUGARCANE BAGASSE

REAKTOR ◽  
2018 ◽  
Vol 18 (03) ◽  
pp. 127 ◽  
Author(s):  
Sita Heris Anita ◽  
Wibowo Mangunwardoyo ◽  
Yopi Yopi
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Physical Properties ◽  
Sugarcane Bagasse ◽  
Immobilized Cells ◽  
Ethanol Yield ◽  
Steam Treatment ◽  
Bioethanol Production ◽  
Whole Cell ◽  
Whole Cell Biocatalyst ◽  
Pretreated Sugarcane Bagasse

Pretreated of sugarcane bagasse was used as a carrier for immobilization of Saccharomyces cerevisiae. Pretreatments were carried out by steaming, pressurized steam, and combination both of procedure.  The objectives of this research was to investigate the effect of pretreatment on sugarcane bagasse to cells adsorption and bioethanol production.  Immobilization process was conducted in a ratio of 2.5 g carrier/50 mL cell suspension.  Whole cell biocatalyst as much as 1% (w/v) was used as inoculum for bioethanol fermentation.  The best pretreated sugarcane bagasse for carrier of immobilized cells was obtained using steam treatment for 30 minutes.  Those treatment improved the physical properties of carrier and increased the cell retention up to 10.05 mg/g.  The use of whole cell biocatalyst after steaming pretreatment also enhanced ethanol yield 1.5 times higher than control. Keywords: bioethanol; immobilization; pretreatment; steam treatment; sugarcane bagasse

Bioethanol Production From Canola Straw Using a Continuous Flow Immobilized Cell System

2012 ◽  
Author(s):  
Anil Mathew ◽  
Mitch Crook ◽  
Keith Chaney ◽  
Andrea Humphries
Keyword(s):  
Continuous Flow ◽  
Immobilized Cells ◽  
Continuous Fermentation ◽  
Energy Generation ◽  
Volumetric Productivity ◽  
Production Costs ◽  
Biodiesel Production ◽  
Bioethanol Production ◽  
Capital Costs ◽  
Canola Straw

Global cultivation of canola increased by approximately 22% between 2000 and 2009, due to increased demand for canola oil for biodiesel production and as an edible oil. In 2009 over 290,000 km2 of canola was cultivated globally. In contrast to oilseed, the commercial market for canola straw is minimal and it is generally ploughed back into the field. The high carbohydrate content (greater than 50 % by dry weight) of canola straw suggests it would be a good feedstock for second-generation bioethanol production. There are four major steps involved in bioethanol production from lignocellulosic materials: (i) pretreatment, (ii) hydrolysis, (iii) fermentation, and (iv) further purification to fuel grade bioethanol through distillation and dehydration. Previous research demonstrated a glucose yield of (440.6 ± 14.9) g kg−1 when canola straw was treated using alkaline pretreatment followed by enzymatic hydrolysis. Whilst bioethanol can be produced using cells free in solution, cell immobilization provides the opportunity to reduce bioethanol production costs by minimizing the extent to which down-stream processing is required, and increasing cellular stability against shear forces. Furthermore, the immobilization process can reduce substrate and product inhibition, which enhances the yield and volumetric productivity of bioethanol production during fermentation, improves operational stability and increases cell viability ensuring cells can be used for several cycles of operation. Previous research used cells of Saccharomyces cerevisiae immobilized in Lentikat® discs to convert glucose extracted from canola straw to bioethanol. In batch mode a yield of (165.1 ± 0.1) g bioethanol kg−1 canola straw was achieved. Continuous fermentation is advantageous in comparison to batch fermentation. The amount of unproductive time (e.g. due to filling, emptying and cleaning) is reduced leading to increased volumetric productivity. The higher volumetric productivity of continuous fermentation means that smaller reactor vessels can be used to produce the same amount of product. This reduces the capital costs associated with a fermentation plant. Research demonstrated a higher bioethanol yield was attained (224.7 g bioethanol kg−1 canola straw) when glucose was converted to bioethanol using immobilized cells in packed-bed continuous flow columns. On an energy generation basis, conversion of 1 kg of canola straw to bioethanol resulted in an energy generation of 6 MJ, representing approximately 35% energy recovery from canola straw. The amount of energy recovered from canola straw could be improved by increasing the amount of energy recovered as bioethanol and by utilising the process by-products in a biorefinery concept.

Production of bioethanol from corn meal hydrolyzates by free and immobilized cells of Saccharomyces cerevisiae var. ellipsoideus

2010 ◽  
Vol 34 (10) ◽  
pp. 1449-1456 ◽  
Author(s):  
Svetlana Nikolić ◽  
Ljiljana Mojović ◽  
Dušanka Pejin ◽  
Marica Rakin ◽  
Maja Vukašinović
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Immobilized Cells ◽  
Corn Meal

scholarly journals Citrus pulp in lamb diets: intake, digestibility, and ruminal parameters

2015 ◽  
Vol 36 (5) ◽  
pp. 3421 ◽  
Author(s):  
Eduardo Lucas Terra Peixoto ◽  
Mirton José Frota Morenz ◽  
Carlos Elysio Moreira da Fonseca ◽  
Elizabeth Dos Santos Moura ◽  
Karla Rodrigues de Lima ◽  
...  
Keyword(s):  
Dry Matter ◽  
Rumen Fluid ◽  
Latin Square ◽  
Neutral Detergent Fiber ◽  
Average Weight ◽  
Corn Meal ◽  
Ruminal Fermentation ◽  
Ruminal Ph ◽  
Citrus Pulp ◽  
Fermentation Parameters

<p>This study aimed to evaluate the viability of replacing corn meal with citrus pulp (0, 25, 50, 75, and 100 % dry matter of corn meal) by evaluating several nutritional parameters such as intake and digestibility of nutrients, and ruminal fermentation parameters. The diets were formulated to be isoproteic with a roughage:concentrate ratio of 60:40. Five crossbred lambs with an initial average weight of 26.1 ± 1.8 kg were used and distributed in a 5 x 5 Latin Square design. For digestibility of nutrients was carried out to feed, orts, and feces collection. The evaluated nutrients were dry matter, crude protein, ether extract, ash, neutral detergent fiber, fiber acid detergent and lignin. Were determined nitrogen and carbohydrate fractions, and ruminal fermentation parameters (N-NH 3 and ruminal pH). The results were subjected to analysis of variance and regression analysis (t-test; ? = 0.05). Citrus pulp inclusion in the diets did not affect intake and digestibility of nutrients, or the pH and the NH3-N content of the rumen fluid. Citrus pulp can be used as a total substitute for corn in concentrate or up to 26.5% in the total ration for lambs (dry basis). </p>

Bioethanol production from mixed sugars by Scheffersomyces stipitis free and immobilized cells, and co-cultures with Saccharomyces cerevisiae

2013 ◽  
Vol 30 (6) ◽  
pp. 591-597 ◽  
Author(s):  
Isabella De Bari ◽  
Paola De Canio ◽  
Daniela Cuna ◽  
Federico Liuzzi ◽  
Angela Capece ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Immobilized Cells ◽  
Bioethanol Production ◽  
Scheffersomyces Stipitis ◽  
Mixed Sugars

scholarly journals A microwave-assisted liquefaction as a pretreatment for the bioethanol production by the simultaneous saccharification and fermentation of corn meal

2008 ◽  
Vol 14 (4) ◽  
pp. 231-234 ◽  
Author(s):  
Svetlana Nikolic ◽  
Ljiljana Mojovic ◽  
Marica Rakin ◽  
Dusanka Pejin ◽  
Dragisa Savic
Keyword(s):  
Simultaneous Saccharification And Fermentation ◽  
Ethanol Concentration ◽  
Microwave Treatment ◽  
Ethanol Productivity ◽  
Bioethanol Production ◽  
Corn Meal ◽  
Microwave Assisted ◽  
Batch System ◽  
Ssf Process ◽  
Simultaneous Saccharification

A microwave-assisted liquefaction as a pretreatment for the bioethanol production by the simultaneous saccharification and fermentation (SSF) of corn meal using Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system was studied. An optimal power of microwaves of 80 W and the 5-min duration of the microwave treatment were selected by following the concentration of glucose released from the corn meal suspensions at hidromodul of 1:3 (corn meal to water ratio) in the liquefaction step. The results indicated that the microwave pretreatment could increase the maximum ethanol concentration produced in the SSF process for 13.4 %. Consequently, a significant increase of the ethanol productivity on substrate (YP/S), as well as the volumetric ethanol productivity (P) in this process, could be achieved.

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