scholarly journals Pim1 knockout alleviates sarcopenia in aging mice via reducing adipogenic differentiation of PDGFRα + mesenchymal progenitors

2021 ◽  
Author(s):  
Guo‐kai Shang ◽  
Lu Han ◽  
Zhi‐hao Wang ◽  
Ming Song ◽  
Di Wang ◽  
...  
Keyword(s):  
Adipogenic Differentiation ◽  
Mesenchymal Progenitors

scholarly journals Mesenchymal Progenitors Derived from Different Locations in Long Bones Display Diverse Characteristics

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Weiguang Lu ◽  
Bo Gao ◽  
Jing Fan ◽  
Pengzhen Cheng ◽  
Yaqian Hu ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Adipogenic Differentiation ◽  
Osteoclast Formation ◽  
Long Bones ◽  
Differentiation Potential ◽  
Mesenchymal Progenitors ◽  
Multiple Differentiation ◽  
Osteoclastic Differentiation ◽  
Proliferation Assays

Mesenchymal progenitors within bone marrow have multiple differentiation potential and play an essential role in the maintenance of adult skeleton homeostasis. Mesenchymal progenitors located in bone regions other than the bone marrow also display bone-forming properties. However, owing to the differences in each distinct microenvironment, the mesenchymal characteristics of skeletal progenitor cells within different regions of long bones may show some differences. In order to clearly elucidate these differences, we performed a comparative study on mesenchymal progenitors from different regions of long bones. Here, we isolated mesenchymal progenitors from the periosteum, endosteum, and bone marrow of rat long bones. The three groups exhibited similar cellular morphologies and expressed the typical surface markers associated with mesenchymal stem cells. Interestingly, after cell proliferation assays and bidirectional differentiation analysis, periosteal mesenchymal progenitors showed a higher proliferative ability and adipogenic differentiation potential. In contrast, endosteal mesenchymal progenitors were more prone to osteogenic differentiation. Using in vitro osteoclast culture systems, conditioned media from different mesenchymal progenitor cultures were used to induce osteoclastic differentiation. Osteoclast formation was found to be significantly promoted by the secretion of RANKL and IL-6 by endosteal progenitors. Overall, our results provide strong evidence for the importance of selecting the appropriate source of skeletal progenitors for applications in future skeleton regeneration therapies.

scholarly journals Loss of Gsα Early in the Osteoblast Lineage Favors Adipogenic Differentiation of Mesenchymal Progenitors and Committed Osteoblast Precursors

2014 ◽  
Vol 29 (11) ◽  
pp. 2414-2426 ◽  
Author(s):  
Partha Sinha ◽  
Piia Aarnisalo ◽  
Rhiannon Chubb ◽  
Noriaki Ono ◽  
Keertik Fulzele ◽  
...  
Keyword(s):  
Adipogenic Differentiation ◽  
Mesenchymal Progenitors ◽  
Osteoblast Precursors ◽  
Osteoblast Lineage

scholarly journals Msx2 Promotes Osteogenesis and Suppresses Adipogenic Differentiation of Multipotent Mesenchymal Progenitors

2003 ◽  
Vol 278 (46) ◽  
pp. 45969-45977 ◽  
Author(s):  
Su-Li Cheng ◽  
Jian-Su Shao ◽  
Nichole Charlton-Kachigian ◽  
Arleen P. Loewy ◽  
Dwight A. Towler
Keyword(s):  
Adipogenic Differentiation ◽  
Mesenchymal Progenitors

scholarly journals Galectin-3 promotes the adipogenic differentiation of PDGFRα+ cells and ectopic fat formation in regenerating muscle

Development ◽  
2022 ◽  
Author(s):  
Naoki Takada ◽  
Masaki Takasugi ◽  
Yoshiki Nonaka ◽  
Tomonori Kamiya ◽  
Kazuaki Takemura ◽  
...  
Keyword(s):  
Cell Population ◽  
Muscle Regeneration ◽  
Adipogenic Differentiation ◽  
Mesenchymal Progenitors ◽  
Intermuscular Adipose Tissue ◽  
Pathological Conditions ◽  
Promising Target ◽  
Galectin 3 ◽  
Prevalence Of Obesity ◽  
Lifestyle Related Diseases

Worldwide prevalence of obesity is associated with the increase of lifestyle-related diseases. The accumulation of intermuscular adipose tissue (IMAT) is considered a major problem whereby obesity leads to sarcopenia and metabolic disorders and thus is a promising target for treating these pathological conditions. However, whereas obesity-associated IMAT is suggested to originate from PDGFRα+ mesenchymal progenitors, processes underlying their adipogenesis remain largely unexplored. Here, we comprehensively investigated intra- and extracellular changes associated with these processes using single-cell RNA sequencing (scRNA-Seq) and mass spectrometry. Our scRNA-Seq analysis identified a small PDGFRα+ cell population in obese mice directed strongly toward adipogenesis. Proteomic analysis showed that the appearance of this cell population is accompanied by an increase in galectin-3 in interstitial environments, which was found to activate adipogenic PPARγ signals in PDGFRα+ cells. Moreover, IMAT formation during muscle regeneration was significantly suppressed in galectin-3 KO mice. Our findings, together with these multi-omics datasets, could unravel microenvironmental networks during muscle regeneration highlighting possible therapeutic targets against IMAT formation in obesity.

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