Oxidative stress in oocyte aging and female reproduction

2021 ◽  
Author(s):  
Ling Wang ◽  
Jinhua Tang ◽  
Lei Wang ◽  
Feng Tan ◽  
Huibin Song ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Female Reproduction ◽  
Oocyte Aging

scholarly journals A novel and compact review on the role of oxidative stress in female reproduction

2018 ◽  
Vol 16 (1) ◽  
Author(s):  
Jiayin Lu ◽  
Zixu Wang ◽  
Jing Cao ◽  
Yaoxing Chen ◽  
Yulan Dong
Keyword(s):  
Oxidative Stress ◽  
Female Reproduction

scholarly journals Applications of Melatonin in Female Reproduction in the Context of Oxidative Stress

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Yonghui Jiang ◽  
Huangcong Shi ◽  
Yue Liu ◽  
Shigang Zhao ◽  
Han Zhao
Keyword(s):  
Oxidative Stress ◽  
Polycystic Ovary ◽  
Oocyte Quality ◽  
Early Embryo ◽  
Female Reproduction ◽  
Adverse Conditions ◽  
Wide Range

Oxidative stress has been recognized as one of the causal mediators of female infertility by affecting the oocyte quality and early embryo development. Improving oxidative stress is essential for reproductive health. Melatonin, a self-secreted antioxidant, has a wide range of effects by improving mitochondrial function and reducing the damage of reactive oxygen species (ROS). This minireview illustrates the applications of melatonin in reproduction from four aspects: physiological ovarian aging, vitrification freezing, in vitro maturation (IVM), and oxidative stress homeostasis imbalance associated with polycystic ovary syndrome (PCOS), emphasising the role of melatonin in improving the quality of oocytes in assisted reproduction and other adverse conditions.

Role of NADPH and hydrogen peroxide in modulating oxidative stress and oocyte aging

2004 ◽  
Vol 82 ◽  
pp. S111
Author(s):  
P.T. Goud ◽  
A.P. Goud ◽  
H.M. Abu-Soud ◽  
M.P. Diamond
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Oocyte Aging

scholarly journals Impact of Cigarette Smoking on the Expression of Oxidative Stress-Related Genes in Cumulus Cells Retrieved from Healthy Women Undergoing IVF

2021 ◽  
Vol 22 (23) ◽  
pp. 13147
Author(s):  
Fani Konstantinidou ◽  
Maria Cristina Budani ◽  
Annalina Sarra ◽  
Liborio Stuppia ◽  
Gian Mario Tiboni ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cigarette Smoking ◽  
Promoter Methylation ◽  
Statistical Significance ◽  
Cumulus Cells ◽  
Female Reproduction ◽  
Stress Genes ◽  
Smoking Exposure ◽  
Stress Related Genes ◽  
The Impact

The female reproductive system represents a sensitive target of the harmful effects of cigarette smoke, with folliculogenesis as one of the ovarian processes most affected by this exposure. The aim of this study was to analyze the impact of tobacco smoking on expression of oxidative stress-related genes in cumulus cells (CCs) from smoking and non-smoking women undergoing IVF techniques. Real time PCR technology was used to analyze the gene expression profile of 88 oxidative stress genes enclosed in a 96-well plate array. Statistical significance was assessed by one-way ANOVA. The biological functions and networks/pathways of modulated genes were evidenced by ingenuity pathway analysis software. Promoter methylation analysis was performed by pyrosequencing. Our results showed a down-regulation of 24 genes and an up-regulation of 2 genes (IL6 and SOD2, respectively) involved in defense against oxidative damage, cell cycle regulation, as well as inflammation in CCs from smoking women. IL-6 lower promoter methylation was found in CCs of the smokers group. In conclusion, the disclosed overall downregulation suggests an oxidant-antioxidant imbalance in CCs triggered by cigarette smoking exposure. This evidence adds a piece to the puzzle of the molecular basis of female reproduction and could help underlay the importance of antioxidant treatments for smoking women undergoing IVF protocols.

scholarly journals Oxidative Stress as Cause, Consequence, or Biomarker of Altered Female Reproduction and Development in the Space Environment

2018 ◽  
Vol 19 (12) ◽  
pp. 3729 ◽  
Author(s):  
Jon Steller ◽  
Jeffrey Alberts ◽  
April Ronca
Keyword(s):  
Oxidative Stress ◽  
Oxidative Damage ◽  
Causative Agent ◽  
Cosmic Radiation ◽  
Developmental Outcomes ◽  
Space Environment ◽  
Reproductive Outcomes ◽  
Female Reproduction ◽  
The Relationship

Oxidative stress has been implicated in the pathophysiology of numerous terrestrial disease processes and associated with morbidity following spaceflight. Furthermore, oxidative stress has long been considered a causative agent in adverse reproductive outcomes. The purpose of this review is to summarize the pathogenesis of oxidative stress caused by cosmic radiation and microgravity, review the relationship between oxidative stress and reproductive outcomes in females, and explore what role spaceflight-induced oxidative damage may have on female reproductive and developmental outcomes.

scholarly journals Determination of oxidative stress balance in follicular fluid

2018 ◽  
Vol 42 (1-2) ◽  
pp. 51-58
Author(s):  
Teja Fabjan ◽  
Eda Vrtačnik-Bokal ◽  
Kristina Kumer ◽  
Joško Osredkar
Keyword(s):  
Oxidative Stress ◽  
Follicular Fluid ◽  
Polycystic Ovary ◽  
Oocyte Retrieval ◽  
Control Group ◽  
Reactive Oxygen Metabolites ◽  
Female Reproduction ◽  
Vitro Fertilization ◽  
Significant Difference

AbstractBackground:The role of oxidative stress in female reproduction is an area that needs more investigation. This study aims to assess the oxidative stress levels in follicular fluid (FF) samples of women undergoing in vitro fertilization (IVF) and to relate them to different diagnoses.Methods:A total of 199 woman were enrolled; 37 of them were diagnosed with polycystic ovary syndrome (PCOS), 71 with endometriosis and 41 with tubal factor infertility (TFI) and 50 of the patients were used as a control group. A sample of FF was collected from each patient at an oocyte retrieval day and analyzed for oxidative stress by measuring a class of reactive oxygen metabolites (ROMs) (dROMs test) and also analyzed for the plasma antioxidant test (PAT).Results:The data analyzed in FF were related to different diagnoses. Groups were not significantly different in age and body mass index (BMI), except for the PCOS group. There was a significant difference between dROMs and PAT levels in FF of patients vs. control group. The same finding was seen when the dROM/PAT ratio was used.Conclusions:We conclude that the evaluation of oxidative stress in FF needs more investigation with regard to markers in the follicular microenvironment.

186 Protective effect of resveratrol on invitro fertilization of ovine oocytes matured under cadmium exposure

2020 ◽  
Vol 32 (2) ◽  
pp. 221
Author(s):  
A. Piras ◽  
F. Ariu ◽  
N. Martino ◽  
A. Maltana ◽  
I. Menéndez-Blanco ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Laser Scanning ◽  
Peripheral Region ◽  
Cadmium Exposure ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Cortical Granules ◽  
Female Reproduction ◽  
Major Mechanism ◽  
Normal Fertilization

Cadmium (Cd) is one of the most toxic environmental and industrial heavy metals, with adverse effect on female reproduction. Earlier invitro studies have demonstrated that exposure to Cd during IVM impairs oocyte fertilization through the induction of oxidative stress (Martino et al. 2017 Reprod. Toxicol. 69, 132-145). The aim of this study was to evaluate the effect of supplementing IVM medium with resveratrol (Res), a phytoalexin with antioxidant activity, on IVF of ovine oocytes matured under Cd exposure and to evaluate whether this effect could be mediated by alterations of cortical granules (CGs) distribution and/or intracellular levels of reactive oxygen species (ROS). Cumulus-oocyte complexes collected from slaughterhouse-derived ovaries of prepubertal ewes were invitro matured with 0μM CdCl2 (control), 2μM CdCl2 (Cd group), or 2μM CdCl2 + 1μM Res (Cd-Res). Groups of MII oocytes were invitro fertilized with ram semen (Bogliolo et al. 2011 Reprod. Fertil. Dev. 23, 809-817). After 16h, oocytes were fixed with absolute ethanol, stained with Hoechst 33342, and classified as follows: normally fertilized (two pronuclei), polyspermic (more than two pronuclei), and abnormally fertilized (asynchronous pronucleus formation). Levels of ROS and distribution of CG in MII oocytes (n=25 each group) were evaluated by confocal laser scanning microscopy after staining with 2’,7’dichlorodihydrofluorescein diacetate and Alexa Fluor 488-conjugated lectin peanut agglutinin, respectively (Jiao et al. 2013 PLoS One 8, 58018). Data of fertilization and CGs distribution were analysed by Chi-squared test and ROS levels (mean±standard error) by Kruskal-Wallis test with STATA\IC 11.0 (Stata Corp.). Oocytes matured in presence of Res had higher normal fertilization and lower polyspermy rates than those matured without Res (Table 1). Levels of ROS in oocytes were lower in the Cd-Res group than in the Cd group (74.4±1.3 vs. 122.4±1.5 pixel/oocyte; P<0.05), and similar to those of the control (71.1±2.3 pixel/oocyte). The ratio of oocytes of Cd-Res group with CGs normally distributed in the peripheral region was higher than that of Cd group (91.3% vs. 65.2%; P<0.05) and comparable to that of control oocytes (95.8%). Collectively, our results showed that resveratrol protects oocytes from Cd-induced alteration of normal fertilization by preventing oxidative stress and improving the major mechanism responsible for blockage of polyspermic fertilization. Table 1.Effect of resveratrol on IVF rate of oocytes matured under cadmium exposure Group No. of total oocytes No. of MII oocytes No. of fertilized oocytes (%) Normal Polyspermy Abnormal Control 136 105 (77.2) 59 (56.2)a 23 (21.9)a 6 (5.7) Cd 146 106 (72.6) 39 (36.8)b 38 (35.8)b 7 (6.6) Cd-Res 114 88 (77.2) 47 (53.4)a 16 (18.2)a 7 (7.9) a,bValues in the same column with different superscripts differ significantly (P<0.05). This project was supported by FDS 2016 (CUP J86C18000780005).

scholarly journals Medicinal Plants and Female Reproduction Disorders due to Oxidative Stress

2020 ◽  
Vol 02 (04) ◽  
Author(s):  
Deutcheu Nienga Sorelle ◽  
Ngoula Ferdinand ◽  
Vemo Bertin Narcisse ◽  
Tchoumboue .
Keyword(s):  
Oxidative Stress ◽  
Medicinal Plants ◽  
Female Reproduction
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