miR‐873‐3p targets HDAC4 to stimulate matrix metalloproteinase‐13 expression upon parathyroid hormone exposure in rat osteoblasts

2020 ◽  
Vol 235 (11) ◽  
pp. 7996-8009 ◽  
Author(s):  
Desai Malavika ◽  
Srinivasan Shreya ◽  
Vembar Raj Priya ◽  
Muthukumar Rohini ◽  
Zhiming He ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Matrix Metalloproteinase ◽  
Matrix Metalloproteinase 13 ◽  
Hormone Exposure ◽  
Rat Osteoblasts

scholarly journals Parathyroid hormone‐induced down‐regulation of miR‐532‐5p for matrix metalloproteinase‐13 expression in rat osteoblasts

2018 ◽  
Vol 119 (7) ◽  
pp. 6181-6193 ◽  
Author(s):  
Vishal Mohanakrishnan ◽  
Arumugam Balasubramanian ◽  
Gokulnath Mahalingam ◽  
Nicola Chennell Partridge ◽  
Ilangovan Ramachandran ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Matrix Metalloproteinase ◽  
Down Regulation ◽  
Matrix Metalloproteinase 13 ◽  
Rat Osteoblasts

Parathyroid hormone increases the expression level of matrix metalloproteinase-13 in vivo

2001 ◽  
Vol 19 (4) ◽  
pp. 207-212 ◽  
Author(s):  
Motoyuki Uchida ◽  
Hideyuki Yamato ◽  
Yumiko Nagai ◽  
Hiroshi Yamagiwa ◽  
Tadashi Hayami ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Matrix Metalloproteinase ◽  
Expression Level ◽  
Matrix Metalloproteinase 13

Nmp4/CIZ regulation of matrix metalloproteinase 13 (MMP-13) response to parathyroid hormone in osteoblasts

2004 ◽  
Vol 287 (2) ◽  
pp. E289-E296 ◽  
Author(s):  
Rita Shah ◽  
Marta Alvarez ◽  
Daniel R. Jones ◽  
Kitti Torrungruang ◽  
Andrew J. Watt ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Matrix Metalloproteinase ◽  
Osteoblast Differentiation ◽  
Matrix Protein ◽  
Type I Collagen ◽  
Zinc Finger Protein ◽  
Regulatory Region ◽  
Type I ◽  
Matrix Metalloproteinase 13 ◽  
Response Region

Parathyroid hormone (PTH) regulation of matrix metalloproteinase-13 ( MMP-13) expression in osteoblasts contributes to normal bone turnover. The PTH response region of the rat MMP-13 gene spans nucleotides (nt) −148 to −38 and supports binding of numerous transcription factors, including Runx2, necessary for osteoblast differentiation, c-Fos/c-Jun, and Ets-1. These trans-acting proteins mediate hormone induction via incompletely defined combinatorial interactions. Within this region, adjacent to the distal Runx2 site, is a homopolymeric(dA:dT) element (−119/−110 nt) that conforms to the consensus site for the novel transcription factor nuclear matrix protein-4/cas interacting zinc finger protein (Nmp4/CIZ). This protein regulates bone cell expression of type I collagen and suppresses BMP2-enhanced osteoblast differentiation. The aim of this study was to determine whether Nmp4/CIZ contributes to MMP-13 basal transcription and PTH responsiveness in osteoblasts. Electrophoretic mobility shift analysis confirms Nmp4/CIZ binding within the MMP-13 PTH response region. Mutation of the Nmp4/CIZ element decreases basal activity of an MMP-13 promoter-reporter construct containing the first 1329 nt of the 5′-regulatory region, and overexpression of Nmp4/CIZ protein enhances the activity of the wild-type promoter. The same mutation of the homopolymeric(dA:dT) element enhances the MMP-13 response to PTH and PGE2. Overexpression of Nmp4/CIZ diminishes hormone induction. Mutation of both the homopolymeric(dA:dT) element and the adjacent Runx2 site further augments the PTH response. On the basis of these data and previous studies, we propose that Nmp4/CIZ is a component of a multiprotein assemblage or enhanceosome within the MMP-13 PTH response region and that, within this context, Nmp4/CIZ promotes both basal expression and hormonal synergy.

scholarly journals HDAC4 Represses Matrix Metalloproteinase-13 Transcription in Osteoblastic Cells, and Parathyroid Hormone Controls This Repression

2010 ◽  
Vol 285 (13) ◽  
pp. 9616-9626 ◽  
Author(s):  
Emi Shimizu ◽  
Nagarajan Selvamurugan ◽  
Jennifer J. Westendorf ◽  
Eric N. Olson ◽  
Nicola C. Partridge
Keyword(s):  
Parathyroid Hormone ◽  
Matrix Metalloproteinase ◽  
Osteoblastic Cells ◽  
Matrix Metalloproteinase 13
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