Circulating microRNAs in patients with suspected acute myocardial infarction: Methodological issues on early diagnostic value

2019 ◽  
Vol 235 (7-8) ◽  
pp. 5856-5857
Author(s):  
Mounesan Leila ◽  
Sabour Siamak
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Diagnostic Value ◽  
Methodological Issues ◽  
Circulating Micrornas ◽  
Suspected Acute Myocardial Infarction ◽  
Early Diagnostic

scholarly journals Plasma miR-22-5p, miR-132-5p, and miR-150-3p Are Associated with Acute Myocardial Infarction

2019 ◽  
Vol 2019 ◽  
pp. 1-13 ◽  
Author(s):  
Huixian Li ◽  
Pengxiang Zhang ◽  
Fangjiang Li ◽  
Guili Yuan ◽  
Xiaoyuan Wang ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Plasma Levels ◽  
Troponin I ◽  
Early Stage ◽  
Diagnostic Value ◽  
Diagnostic Efficacy ◽  
Diagnostic Biomarkers ◽  
Circulating Micrornas ◽  
Novel Biomarkers

Circulating microRNAs (miRNAs) are potential biomarkers for cardiovascular diseases. Our study aimed to determine whether miR-22-5p, miR-132-5p, and miR-150-3p represent novel biomarkers for acute myocardial infarction (AMI). Plasma samples were isolated from 35 AMI patients and 55 matched controls. Total RNA was extracted, and quantitative real-time PCR and ELISA were performed to investigate the expressions of miRNAs and cardiac troponin I (cTnI), respectively. We found that plasma levels of miR-22-5p and miR-150-3p were significantly higher during the early stage of AMI and their expression levels peaked earlier than cTnI. Conversely, circulating miR-132-5p was sustained at a low level during the early phase of AMI. All three circulating miRNAs were correlated with plasma cTnI levels. A receiver operating characteristic (ROC) analysis suggested that each single miRNA had considerable diagnostic efficacy for AMI. Moreover, combining the three miRNAs improved their diagnostic efficacy. Furthermore, neither heparin nor medications for coronary heart disease (CHD) affected plasma levels of miR-22-5p and miR-132-5p, but circulating miR-150-3p was downregulated by medications for CHD. We concluded that plasma miR-22-5p, miR-132-5p, and miR-150-3p may serve as candidate diagnostic biomarkers for early diagnosis of AMI. Moreover, a panel consisting of these three miRNAs may achieve a higher diagnostic value.

Serum LD-1 activity in suspected acute myocardial infarction.

1983 ◽  
Vol 29 (6) ◽  
pp. 1057-1060 ◽  
Author(s):  
W Gerhardt ◽  
S Hofvendahl ◽  
L Ljungdahl ◽  
J Waldenström ◽  
N Tryding ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Lactate Dehydrogenase ◽  
Diagnostic Value ◽  
Suspected Acute Myocardial Infarction

Abstract We have studied the diagnostic value of measuring lactate dehydrogenase (LD) isoenzyme 1 in serum of 331 cases of suspected acute myocardial infarction (AMI). At a discriminatory level of 200 U/L (Scandinavian Committee on Enzymes, recommended method for the determination of LD) LD 1 verified the diagnosis in 96% of the AMI cases and excluded it in 96% of the not-AMI cases when samples were drawn 24-72 h after onset of pain. The correlation between 24-h S-LD-1 and 16-h S-CK B activities was 0.94 in the AMI cases. We found that quantitation of serum LD-1 is diagnostically more reliable than the serum LD-1/LD ratio.

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