Immunomodulatory activity of Ganoderma lucidum immunomodulatory protein via PI3K/Akt and MAPK signaling pathways in RAW264.7 cells

2019 ◽  
Vol 234 (12) ◽  
pp. 23337-23348 ◽  
Author(s):  
Qi‐Zhang Li ◽  
Yu‐Zhou Chang ◽  
Zhu‐Mei He ◽  
Lei Chen ◽  
Xuan‐Wei Zhou
Keyword(s):  
Signaling Pathways ◽  
Ganoderma Lucidum ◽  
Mapk Signaling ◽  
Raw264.7 Cells ◽  
Immunomodulatory Activity ◽  
Immunomodulatory Protein ◽  
Mapk Signaling Pathways

scholarly journals Immunomodulatory activity of Ganoderma lucidum immunomodulatory protein via PI3K/Akt and MAPK signaling pathways in macrophage RAW264.7 cells

2018 ◽  
Author(s):  
Xuanwei Zhou ◽  
Qizhang Li ◽  
Yuzhou Chang ◽  
Liu-Ding-Ji Li ◽  
Xiaoyu Du ◽  
...  
Keyword(s):  
Ganoderma Lucidum ◽  
Kinase Inhibitor ◽  
Mapk Signaling ◽  
Raw264.7 Cells ◽  
In Vitro Assays ◽  
Immunomodulatory Activity ◽  
Inos Mrna ◽  
Sequencing Analysis ◽  
Mrna Levels ◽  
Immunomodulatory Protein

Ganoderma lucidum, a traditional edible and medicinal fungus, holds an important status in health care systems in China and other Asian countries. Fungal immunomodulatory protein (FIP), one of the active ingredients isolated from G. lucidum, is a class of naturally occurring proteins and possesses potential biological functions. This study was conducted to explore the molecular mechanism of its immunomodulatory potency in immune responses of macrophages. In vitro assays of biological activity indicated that rFIP-glu significantly activated macrophage RAW264.7 cells, and possessed the ability of pro- and anti-inflammation the cells. RNA sequencing analysis showed that macrophage activation involved Toll-like receptors and mitogen-activated protein kinases pathways. Furthermore, qRT-PCR indicated that phosphoinositide 3 kinase inhibitor LY294002 blocked the mRNA levels of MCP-1, MEK1/2 inhibitor U0126 reduced the mRNA levels of TNF-α and MCP-1, and JNK inhibitor SP600125 prevented the up-regulation of iNOS mRNA in the rFIP-glu-induced cells. FIP-glu mediated these inflammatory effects not through a general pathway, instead through a different pathway for different inflammatory mediator. These data indicate the possibility that rFIP-glu has an important immune-regulation function and thus has potential therapeutic uses.

Ganoderma lucidum polysaccharide inhibits UVB-induced melanogenesis by antagonizing cAMP/PKA and ROS/MAPK signaling pathways

2018 ◽  
Vol 234 (5) ◽  
pp. 7330-7340 ◽  
Author(s):  
Shuanghai Hu ◽  
Jinhua Huang ◽  
Shiyao Pei ◽  
Yujie Ouyang ◽  
Yufang Ding ◽  
...  
Keyword(s):  
Signaling Pathways ◽  
Ganoderma Lucidum ◽  
Mapk Signaling ◽  
Mapk Signaling Pathways

scholarly journals Frutescone O from Baeckea frutescens Blocked TLR4-Mediated Myd88/NF-κB and MAPK Signaling Pathways in LPS Induced RAW264.7 Macrophages

2021 ◽  
Vol 12 ◽  
Author(s):  
Xiaobing Lin ◽  
Junhan Zhang ◽  
Decai Fan ◽  
Jiqin Hou ◽  
Hao Wang ◽  
...  
Keyword(s):  
Signaling Pathways ◽  
Molecular Mechanisms ◽  
Mapk Signaling ◽  
Raw264.7 Cells ◽  
Raw264.7 Macrophages ◽  
Anti Inflammatory ◽  
Baeckea Frutescens ◽  
Myd88 Signaling ◽  
Myeloid Differentiation Factor ◽  
Mapk Signaling Pathways

Frutescone O was isolated from the aerial parts of Baeckea frutescens L., which was commonly used as a folk medicinal material for treating anti-inflammatory disease in South East Asia. This study aimed to investigate the anti-inflammatory activity and related signaling cascade of Frutescone O (Fru) in LPS induced RAW264.7 cells. The anti-inflammation activity of Frutescone O was determined according to the inhibitory effects on the secretion of nitric oxide (NO), expression of inducible NO synthase, and pro-inflammatory cytokines. The regulation of Myeloid differentiation factor 88 (Myd88), inhibition of NF-κB, and MAPK pathways were further investigated for molecular mechanisms. Fru significantly decreased the expression of iNOS and the production of NO in LPS-stimulated RAW264.7 cells. It also dose-dependently suppressed LPS induced expression of IL-1β, IL-6, and TNF-α. Furthermore, Fru remarkably inhibited the upregulation of NF-κB (p50) expression in the nucleus and the phosphorylation ratio of p38, JNK, ERK, and Myd88 signaling protein. The molecular docking and cellular thermal shift assay (CETSA) results indicated that Fru participated in a robust and stable interaction with the active site of TLR4-MD2. Thus, Fru suppressed the LPS induced inflammation in RAW264.7 cells by blocking the TLR4 mediated signal transduction through the NF-κB and MAPK signaling pathways and inhibiting the Myd88 and iNOS expression.

Macrophage-stimulating activity of European eel (Anguilla anguilla) peptides in RAW264.7 cells mediated via NF-κB and MAPK signaling pathways

2020 ◽  
Vol 11 (12) ◽  
pp. 10968-10978
Author(s):  
Lijun Yao ◽  
Peng Yang ◽  
Wenqi Luo ◽  
Shuiming Li ◽  
Yan Wu ◽  
...  
Keyword(s):  
Signaling Pathways ◽  
Anguilla Anguilla ◽  
Mapk Signaling ◽  
Raw264.7 Cells ◽  
European Eel ◽  
Immunostimulatory Effect ◽  
Effective Component ◽  
Mapk Signaling Pathways

This is the first study to investigate the immunostimulatory effect of eel peptide (EP) from Anguilla anguilla in RAW264.7 cells, and the most effective component of EP mainly consisted of collagen-derived Hyp-containing peptides.

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