Combinational therapy of lithium and human neural stem cells in rat spinal cord contusion model

2019 ◽  
Vol 234 (11) ◽  
pp. 20742-20754 ◽  
Author(s):  
Atiyeh Mohammadshirazi ◽  
Hoda Sadrosadat ◽  
Razieh Jaberi ◽  
Masoomeh Zareikheirabadi ◽  
Sara Mirsadeghi ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Neural Stem Cells ◽  
Rat Spinal Cord ◽  
Human Neural Stem Cells ◽  
Combinational Therapy ◽  
Spinal Cord Contusion

Recovery of CNS Pathway Innervating the Sciatic Nerve Following Transplantation of Human Neural Stem Cells in Rat Spinal Cord Injury

2011 ◽  
Vol 32 (1) ◽  
pp. 149-157 ◽  
Author(s):  
Kwang-Bok Lee ◽  
Jung Hoon Choi ◽  
Kyunghee Byun ◽  
Kwang Hoon Chung ◽  
Ji Hyeon Ahn ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Spinal Cord Injury ◽  
Sciatic Nerve ◽  
Neural Stem Cells ◽  
Rat Spinal Cord ◽  
Human Neural Stem Cells ◽  
Cord Injury

scholarly journals Immunosuppressants Affect Human Neural Stem Cells In Vitro but Not in an In Vivo Model of Spinal Cord Injury

2013 ◽  
Vol 2 (10) ◽  
pp. 731-744 ◽  
Author(s):  
Christopher J. Sontag ◽  
Hal X. Nguyen ◽  
Noriko Kamei ◽  
Nobuko Uchida ◽  
Aileen J. Anderson ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Spinal Cord Injury ◽  
Neural Stem Cells ◽  
In Vivo Model ◽  
Human Neural Stem Cells ◽  
Cord Injury

Targeted Treatment of Experimental Spinal Cord Glioma With Dual Gene-Engineered Human Neural Stem Cells

Neurosurgery ◽  
2015 ◽  
Vol 79 (3) ◽  
pp. 481-491 ◽  
Author(s):  
Alexander E. Ropper ◽  
Xiang Zeng ◽  
Hariprakash Haragopal ◽  
Jamie E. Anderson ◽  
Zaid Aljuboori ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Growth Rate ◽  
Neural Stem Cells ◽  
Tumor Growth ◽  
Rat Model ◽  
Weight Bearing ◽  
Tumor Growth Rate ◽  
Intramedullary Spinal Cord ◽  
Human Neural Stem Cells

Abstract BACKGROUND There are currently no satisfactory treatments or experimental models showing autonomic dysfunction for intramedullary spinal cord gliomas (ISCG). OBJECTIVE To develop a rat model of ISCG and investigate whether genetically engineered human neural stem cells (F3.hNSCs) could be developed into effective therapies for ISCG. METHODS Immunodeficient/Rowett Nude rats received C6 implantation of G55 human glioblastoma cells (10K/each). F3.hNSCs engineered to express either cytosine deaminase gene only (i.e., F3.CD) or dual genes of CD and thymidine kinase (i.e., F3.CD-TK) converted benign 5-fluorocytosine and ganciclovir into oncolytic 5-fluorouracil and ganciclovir-triphosphate, respectively. ISCG rats received injection of F3.CD-TK, F3.CD, or F3.CD-TK debris near the tumor epicenter 7 days after G55 seeding, followed with 5-FC (500 mg/kg/5 mL) and ganciclovir administrations (25 mg/kg/1 mL/day × 5/each repeat, intraperitoneal injection). Per humane standards for animals, loss of weight-bearing stepping in the hindlimb was used to determine post-tumor survival. Also evaluated were autonomic functions and tumor growth rate in vivo. RESULTS ISCG rats with F3.CD-TK treatment survived significantly longer (37.5 ± 4.78 days) than those receiving F3.CD (21.5 ± 1.75 days) or F3.CD-TK debris (19.3 ± 0.85 days; n = 4/group; P <.05, median rank test), with significantly improved autonomic function and reduced tumor growth rate. F3.DC-TK cells migrated diffusively into ISCG clusters to mediate oncolytic effect. CONCLUSION Dual gene-engineered human neural stem cell regimen markedly prolonged survival in a rat model that emulates somatomotor and autonomic dysfunctions of human cervical ISCG. F3.CD-TK may provide a novel approach to treating clinical ISCG.

Engraftment, migration and differentiation of neural stem cells in the rat spinal cord following contusion injury

Cytotherapy ◽  
2010 ◽  
Vol 12 (3) ◽  
pp. 313-325 ◽  
Author(s):  
Siobhan S. McMahon ◽  
Silke Albermann ◽  
Gemma E. Rooney ◽  
Georgina Shaw ◽  
Yolanda Garcia ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Neural Stem Cells ◽  
Rat Spinal Cord ◽  
Contusion Injury

Astrocytes in injured adult rat spinal cord may acquire the potential of neural stem cells

Neuroscience ◽  
2004 ◽  
Vol 128 (4) ◽  
pp. 775-783 ◽  
Author(s):  
B. Lang ◽  
H.L. Liu ◽  
R. Liu ◽  
G.D. Feng ◽  
X.Y. Jiao ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Neural Stem Cells ◽  
Rat Spinal Cord ◽  
Adult Rat ◽  
Adult Rat Spinal Cord
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