Biomechanical properties of red blood cells infected by Plasmodium berghei ANKA

2019 ◽  
Vol 234 (11) ◽  
pp. 20546-20553 ◽  
Author(s):  
Sangwoo Kwon ◽  
Dong‐Hun Lee ◽  
Se‐Jik Han ◽  
Woochul Yang ◽  
Fu‐Shi Quan ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Plasmodium Berghei ◽  
Blood Cells ◽  
Biomechanical Properties ◽  
Plasmodium Berghei Anka

scholarly journals Comparative assessment of Chemosuppressive activity of three Chinese teas on Plasmodium berghei infected mice

2018 ◽  
Vol 7 (4) ◽  
pp. 376-383
Author(s):  
Bankole Olukayode Olusola ◽  
◽  
Oderinde Abdulganiyu Olumuyiwa ◽  
Keyword(s):  
Body Weight ◽  
Red Blood Cells ◽  
Plasmodium Berghei ◽  
Blood Cells ◽  
Haemoglobin Concentration ◽  
Treated Group ◽  
Antimalarial Drugs ◽  
Comparative Assessment ◽  
Haematological Parameters ◽  
Weight Changes

Malaria, a hazardous infirmity caused by a parasitic malady of the red blood cells, is without question harming to the wellbeing. In the present investigation, the chemosuppresive and haematopoietic activities of 200 mg/kg and 400 mg/kg body weight of unrefined ethanolic concentrates of three Chinese green teas (BIA 849, TD 570 and GB/T19598) were assessed using the 4-day suppressive anti-plasmodial assay in mice Plasmodium berghei (NK65 strain) pre-infected mice. The effect of the extracts on weight of the animals was evaluated. It was observed that 200 mg/kg bw (body weight) of BIA 849 and GB/T19598 were as potent as 5 mg/kg bw of chloroquine, with percentage suppressions of 58.97 ± 5.04, 57.63 ± 5.62 and 57.50 ± 4.5, respectively. TD570 at 200 mg/kg bw was more effective in suppressing plasmodium. 400 mg/kg body weight of TD570 and GB/T19598 extracts were more potent than 5 mg/kg bw of chloroquine having 100 % chemosuppression. The chemosuppression of BIA 849 did not change altogether at 400 mg/kg bw. The haematological parameters, WBC, RBC and MCV did not significantly change in the groups treated with the tea extracts utilizing suppressive model of malaria treatment contrasted with the uninfected group and were comparable to those treated with chloroquine. Haemoglobin concentration nonetheless, varied significantly with respect to the uninfected group. Weight changes were most significant with 200 mg/kg bw of TD 570 treated group (32 % increase) on suppression. All in all, the green teas displayed high chemosuppressive and haematopoietic possibilities and are thusly prescribed as contender for additionally screening as elective antimalarial drugs

Superoxide Dismutase (SOD) in Mouse Red Blood Cells Infected with Plasmodium berghei

1982 ◽  
Vol 68 (2) ◽  
pp. 337 ◽  
Author(s):  
Upsorn Suthipark ◽  
Jerapan Krungkrai ◽  
Amornrat Jearnpipatkul ◽  
Yongyuth Yuthavong ◽  
Bhinyo Panijpan
Keyword(s):  
Superoxide Dismutase ◽  
Red Blood Cells ◽  
Plasmodium Berghei ◽  
Blood Cells

Plasmodium berghei-infected red cells sorted according to DNA content

Parasitology ◽  
1979 ◽  
Vol 78 (3) ◽  
pp. 263-270 ◽  
Author(s):  
R. J. Howard ◽  
F. L. Battye
Keyword(s):  
Red Blood Cells ◽  
Dna Content ◽  
Cell Sorting ◽  
Plasmodium Berghei ◽  
Blood Cells ◽  
Dye Uptake ◽  
Direct Proportion ◽  
Mouse Blood ◽  
Infected Blood ◽  
A Cell

SUMMARYA cell-sorting method is described for the analysis and separation of red blood cells in Plasmodium berghei-infected mouse blood based on their DNA content. This method involves a selective uptake of the bis-benzimidazole dye 33258 Hoechst, a DNA-binding dye, by red blood cells containing parasites. Infected blood is incubated at 37 °C with the dye then washed at 4 °C to remove unbound dye. Uninfected cells are then non-fluorescent at the characteristic wavelengths for 33258 Hoechst excitation and emission, whereas parasitized cells display fluorescence intensities in approximately direct proportion to the number of parasite nuclei (i.e. amount of parasite DNA) within the cell and can be sorted accordingly. Providing cells were incubated in a complex nutrient medium during dye uptake at 37°C, the sorted parasite cells produced lethal P. berghei infections when injected into BALB/c mice. The dyelabelling technique is simple and sufficient red blood cells at various stages of infection can be collected for biochemical or immunochemical studies by cell sorting.

Establishment of a murine model of cerebral malaria in KunMing mice infected with Plasmodium berghei ANKA

Parasitology ◽  
2016 ◽  
Vol 143 (12) ◽  
pp. 1672-1680 ◽  
Author(s):  
YAN DING ◽  
WENYUE XU ◽  
TAOLI ZHOU ◽  
TAIPING LIU ◽  
HONG ZHENG ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Cerebral Malaria ◽  
Plasmodium Berghei ◽  
Blood Cells ◽  
Mononuclear Cells ◽  
Clinical Signs ◽  
Necrosis Factor Alpha ◽  
Experimental Cerebral Malaria ◽  
Km Mice

SUMMARYMalaria remains one of the most devastating diseases. Cerebral malaria (CM) is a severe complication of Plasmodium falciparum infection resulting in high mortality and morbidity worldwide. Analysis of precise mechanisms of CM in humans is difficult for ethical reasons and animal models of CM have been employed to study malaria pathogenesis. Here, we describe a new experimental cerebral malaria (ECM) model with Plasmodium berghei ANKA infection in KunMing (KM) mice. KM mice developed ECM after blood-stage or sporozoites infection, and the development of ECM in KM mice has a dose-dependent relationship with sporozoites inoculums. Histopathological findings revealed important features associated with ECM, including accumulation of mononuclear cells and red blood cells in brain microvascular, and brain parenchymal haemorrhages. Blood–brain barrier (BBB) examination showed that BBB disruption was present in infected KM mice when displaying clinical signs of CM. In vivo bioluminescent imaging experiment indicated that parasitized red blood cells accumulated in most vital organs including heart, lung, spleen, kidney, liver and brain. The levels of inflammatory cytokines interferon-gamma, tumour necrosis factor-alpha, interleukin (IL)-17, IL-12, IL-6 and IL-10 were all remarkably increased in KM mice infected with P. berghei ANKA. This study indicates that P. berghei ANKA infection in KM mice can be used as ECM model to extend further research on genetic, pharmacological and vaccine studies of CM.

scholarly journals Amodiaquine and Ciprofloxacin Combination in Plasmodiasis Therapy

2015 ◽  
Vol 2015 ◽  
pp. 1-4 ◽  
Author(s):  
Peace Mayen Edwin Ubulom ◽  
Chinweizu Ejikeme Udobi ◽  
Mark Iheukwumere Madu
Keyword(s):  
Body Weight ◽  
Combination Therapy ◽  
Red Blood Cells ◽  
Plasmodium Berghei ◽  
Blood Cells ◽  
Dosage Level ◽  
Recommended Dosage ◽  
Swiss Albino Mice ◽  
Better Than

Objective. The study was designed to determine the efficacy of combined Amodiaquine and Ciprofloxacin in plasmodiasis therapy.Method. The in vivo antiplasmodial effect of different dosage levels of Amodiaquine, Ciprofloxacin, and their combinations againstPlasmodium berghei bergheiwas evaluated using Swiss albino mice.Results. Amodiaquine (a known antiplasmodial agent) had a fairly significant antiplasmodial effect reducing the parasites for every 100 red blood cells (RBC) from 66 to 16 (75.75%) at the tolerable dosage level of 7.5 mg/kg body weight while Ciprofloxacin (an antibiotic known to have antimalarial effect) showed an insignificant antiplasmodial effect reducing the parasites for every 100 RBC from 65 to 64 (1.53%) at the tolerable dosage level of 10.7 mg/kg body weight. Conversely, the combination therapy of Amodiaquine and Ciprofloxacin had a significant antiplasmodial effect at all the doses administered. The combination of 7.5 mg/kg of Amodiaquine and 12.8 mg/kg of Ciprofloxacin, however, showed the most significant antiplasmodial effect of the doses used reducing the number of parasites per 100 RBC from 60 to 10 (83.33%).Conclusions. Appropriate Amodiaquine and Ciprofloxacin combination will be effective for the treatment of malaria and better than either Amodiaquine or Ciprofloxacin singly at their recommended dosage levels.

Lipid content of Plasmodium berghei-infected rat red blood cells

1969 ◽  
Vol 26 (2) ◽  
pp. 181-186 ◽  
Author(s):  
C.W. Lawrence ◽  
Richard J. Cenedella
Keyword(s):  
Red Blood Cells ◽  
Lipid Content ◽  
Plasmodium Berghei ◽  
Blood Cells

Flow cytometric enumeration of Plasmodium berghei-infected red blood cells stained with SYBR Green I

Acta Tropica ◽  
2012 ◽  
Vol 122 (1) ◽  
pp. 113-118 ◽  
Author(s):  
Voravuth Somsak ◽  
Somdet Srichairatanakool ◽  
Yongyuth Yuthavong ◽  
Sumalee Kamchonwongpaisan ◽  
Chairat Uthaipibull
Keyword(s):  
Red Blood Cells ◽  
Plasmodium Berghei ◽  
Blood Cells ◽  
Sybr Green ◽  
Sybr Green I ◽  
Flow Cytometric

scholarly journals Cytoadherence of Plasmodium berghei-Infected Red Blood Cells to Murine Brain and Lung Microvascular Endothelial CellsIn Vitro

2013 ◽  
Vol 81 (11) ◽  
pp. 3984-3991 ◽  
Author(s):  
Fatima El-Assaad ◽  
Julie Wheway ◽  
Andrew John Mitchell ◽  
Jinning Lou ◽  
Nicholas Henry Hunt ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Red Blood Cells ◽  
Plasmodium Berghei ◽  
Blood Cells ◽  
Content Type ◽  
Murine Brain ◽  
Microvascular Endothelial ◽  
Mixed Stage

ABSTRACTSequestration of infected red blood cells (iRBC) within the cerebral and pulmonary microvasculature is a hallmark of human cerebral malaria (hCM). The interaction between iRBC and the endothelium in hCM has been studied extensively and is linked to the severity of malaria. Experimental CM (eCM) caused byPlasmodium bergheiANKA reproduces most features of hCM, although the sequestration of RBC infected byP. bergheiANKA (PbA-iRBC) has not been completely delineated. The role of PbA-iRBC sequestration in the severity of eCM is not well characterized. Using static and flow cytoadherence assays, we provide the first directin vitroevidence for the binding of PbA-iRBC to murine brain and lung microvascular endothelial cells (MVEC). We found that basal PbA-iRBC cytoadherence to MVECs was significantly higher than that of normal red blood cells (NRBC) and of RBC infected withP. bergheiK173 (PbK173-iRBC), a strain that causes noncerebral malaria (NCM). MVEC prestimulation with tumor necrosis factor (TNF) failed to promote any further significant increase in mixed-stage iRBC adherence. Interestingly, enrichment of the blood for mature parasites significantly increased PbA-iRBC binding to the MVECs prestimulated with TNF, while blockade of VCAM-1 reduced this adhesion. Our study provides evidence for the firm, flow-resistant binding to endothelial cells of iRBC from strain ANKA-infected mice, which develop CM, and for less binding of iRBC from strain K173-infected mice, which develop NCM. An understanding ofP. bergheicytoadherence may help elucidate the importance of sequestration in the development of CM and aid the development of antibinding therapies to help reduce the burden of this syndrome.

Autoantibodies to Red Blood Cells in Rats Infected with Plasmodium berghei

1981 ◽  
Vol 67 (3) ◽  
pp. 351 ◽  
Author(s):  
Z. Ronai ◽  
H. Avraham ◽  
D. Sulitzeanu
Keyword(s):  
Red Blood Cells ◽  
Plasmodium Berghei ◽  
Blood Cells

scholarly journals Reduced CD36-dependent tissue sequestration of Plasmodium-infected erythrocytes is detrimental to malaria parasite growth in vivo

2011 ◽  
Vol 209 (1) ◽  
pp. 93-107 ◽  
Author(s):  
Jannik Fonager ◽  
Erica M. Pasini ◽  
Joanna A.M. Braks ◽  
Onny Klop ◽  
Jai Ramesar ◽  
...  
Keyword(s):  
Vascular Endothelium ◽  
Plasmodium Berghei ◽  
Blood Cells ◽  
Malaria Infection ◽  
Plasmodium Falciparum Malaria ◽  
Parasite Growth ◽  
Plasmodium Berghei Anka ◽  
Parasite Plasmodium ◽  
First Time

Adherence of parasite-infected red blood cells (irbc) to the vascular endothelium of organs plays a key role in the pathogenesis of Plasmodium falciparum malaria. The prevailing hypothesis of why irbc adhere and sequester in tissues is that this acts as a mechanism of avoiding spleen-mediated clearance. Irbc of the rodent parasite Plasmodium berghei ANKA sequester in a fashion analogous to P. falciparum by adhering to the host receptor CD36. To experimentally determine the significance of sequestration for parasite growth, we generated a mutant P. berghei ANKA parasite with a reduced CD36-mediated adherence. Although the cognate parasite ligand binding to CD36 is unknown, we show that nonsequestering parasites have reduced growth and we provide evidence that in addition to avoiding spleen removal, other factors related to CD36-mediated sequestration are beneficial for parasite growth. These results reveal for the first time the importance of sequestration to a malaria infection, with implications for the development of strategies aimed at reducing pathology by inhibiting tissue sequestration.

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