scholarly journals Differential effects of antiepileptic drugs on human bone cells

2019 ◽  
Vol 234 (11) ◽  
pp. 19691-19701 ◽  
Author(s):  
Sara Rocha ◽  
Ricardo Ferraz ◽  
Cristina Prudêncio ◽  
Maria Helena Fernandes ◽  
João Costa‐Rodrigues
Keyword(s):  
Antiepileptic Drugs ◽  
Bone Cells ◽  
Human Bone ◽  
Differential Effects

scholarly journals Inhibition of glypican-1 expression induces an activated fibroblast phenotype in a human bone marrow-derived stromal cell-line

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sukhneeraj P. Kaur ◽  
Arti Verma ◽  
Hee. K. Lee ◽  
Lillie M. Barnett ◽  
Payaningal R. Somanath ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Bone Marrow ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Stromal Cell ◽  
Prostate Cancer Cell ◽  
Bone Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Prostate Cancer Cells

AbstractCancer-associated fibroblasts (CAFs) are the most abundant stromal cell type in the tumor microenvironment. CAFs orchestrate tumor-stromal interactions, and contribute to cancer cell growth, metastasis, extracellular matrix (ECM) remodeling, angiogenesis, immunomodulation, and chemoresistance. However, CAFs have not been successfully targeted for the treatment of cancer. The current study elucidates the significance of glypican-1 (GPC-1), a heparan sulfate proteoglycan, in regulating the activation of human bone marrow-derived stromal cells (BSCs) of fibroblast lineage (HS-5). GPC-1 inhibition changed HS-5 cellular and nuclear morphology, and increased cell migration and contractility. GPC-1 inhibition also increased pro-inflammatory signaling and CAF marker expression. GPC-1 induced an activated fibroblast phenotype when HS-5 cells were exposed to prostate cancer cell conditioned media (CCM). Further, treatment of human bone-derived prostate cancer cells (PC-3) with CCM from HS-5 cells exhibiting GPC-1 loss increased prostate cancer cell aggressiveness. Finally, GPC-1 was expressed in mouse tibia bone cells and present during bone loss induced by mouse prostate cancer cells in a murine prostate cancer bone model. These data demonstrate that GPC-1 partially regulates the intrinsic and extrinsic phenotype of human BSCs and transformation into activated fibroblasts, identify novel functions of GPC-1, and suggest that GPC-1 expression in BSCs exerts inhibitory paracrine effects on the prostate cancer cells. This supports the hypothesis that GPC-1 may be a novel pharmacological target for developing anti-CAF therapeutics to control cancer.

scholarly journals The novel endolysin XZ.700 effectively treats MRSA biofilms in two biofilm models without showing toxicity on human bone cells in vitro

Biofouling ◽  
2021 ◽  
pp. 1-10
Author(s):  
Jesse W. P Kuiper ◽  
Jolanda M. A Hogervorst ◽  
Bjorn L. Herpers ◽  
Astrid D. Bakker ◽  
Jenneke Klein-Nulend ◽  
...  
Keyword(s):  
Bone Cells ◽  
Human Bone ◽  
The Novel

Differential effects of antiepileptic drugs on sexual function and hormones in men with epilepsy

Neurology ◽  
2005 ◽  
Vol 65 (7) ◽  
pp. 1016-1020 ◽  
Author(s):  
A. G. Herzog ◽  
F. W. Drislane ◽  
D. L. Schomer ◽  
P. B. Pennell ◽  
E. B. Bromfield ◽  
...  
Keyword(s):  
Sexual Function ◽  
Antiepileptic Drugs ◽  
Differential Effects

Effects of fluoride on human bone cells in vitro: differences in responsiveness between stromal osteoblast precursors and mature osteoblasts

1994 ◽  
Vol 130 (4) ◽  
pp. 381-386 ◽  
Author(s):  
Moustapha Kassem ◽  
Leif Mosekilde ◽  
Erik F Eriksen
Keyword(s):  
Bone Marrow ◽  
Alkaline Phosphatase ◽  
Sodium Fluoride ◽  
Bone Cells ◽  
Human Bone ◽  
Type I ◽  
Procollagen Type ◽  
Procollagen Type I ◽  
Osteoblast Precursors

Kassem M, Mosekilde L, Eriksen EF. Effects of fluoride on human bone cells in vitro: differences in responsiveness between stromal osteoblast precursors and mature osteoblasts. Eur J Endocrinol 1994;130:381–6. ISSN 0804–4643 The cellular effects of sodium fluoride (NaF) on human bone cells in vitro have been variable and dependent on the culture system used. Variability could be attributed to differences in responsiveness to NaF among different populations of cells at various stages of differentiation in the osteoblastic lineage. In this study we compared the effects of NaF in serum-free medium on cultures of more differentiated human osteoblast-like (hOB) cells derived from trabecular bone explants and on osteoblast committed precursors derived from human bone marrow, i.e. human marrow stromal osteoblast-like (hMS(OB)) cells. Sodium fluoride (10−5 mol/l) increased proliferation of hMS(OB) cells (p<0.05, N = 10) but was not mitogenic to hOB cells (p>0.05, N= 10). Alkaline phosphatase (AP) production increased in both hMS(OB) (p<0.05, N=9) and hOB cells (p<0.05, N=9). No significant effects on procollagen type I propeptide production were obtained in either culture. In the presence of 1,25-dihydroxycholecalciferol (10−9 mol/l), NaF enhanced alkaline phosphatase (p<0.05, N=8), procollagen type I propeptide (p<0.05, N=7) and osteocalcin (p<0.05, N=7) production by hMS(OB) cells but not by hOB cells. Our results suggest that osteoblast precursors are more sensitive to NaF action than mature osteoblasts and that the in vivo effects of NaF on bone formation may be mediated by stimulating proliferation and differentiation of committed osteoblast precursors in bone marrow. M Kassem, Mayo Clinic, Endocrine Research Unit, W-Joseph 5-164, Rochester, MN 55904, USA

P27. Proliferative effect of vitamin D metabolites on human bone cells

Bone ◽  
1989 ◽  
Vol 10 (6) ◽  
pp. 483
Author(s):  
DB Evans ◽  
M Thavarajah ◽  
JA Kanis
Keyword(s):  
Vitamin D ◽  
Bone Cells ◽  
Human Bone ◽  
Vitamin D Metabolites ◽  
Proliferative Effect

Androgen receptor isoforms AR-A and AR-B display functional differences in cultured human bone cells and genital skin fibroblasts

Steroids ◽  
2003 ◽  
Vol 68 (14) ◽  
pp. 1179-1187 ◽  
Author(s):  
Ute M. Liegibel ◽  
Ulrike Sommer ◽  
Irma Boercsoek ◽  
Ulrike Hilscher ◽  
Angelika Bierhaus ◽  
...  
Keyword(s):  
Androgen Receptor ◽  
Bone Cells ◽  
Skin Fibroblasts ◽  
Human Bone ◽  
Functional Differences ◽  
Receptor Isoforms

Effect of Angelica sinensis on the proliferation of human bone cells

2002 ◽  
Vol 324 (1-2) ◽  
pp. 89-97 ◽  
Author(s):  
Qing Yang ◽  
Stephen M Populo ◽  
Jianying Zhang ◽  
Guoguang Yang ◽  
Hiroyuki Kodama
Keyword(s):  
Bone Cells ◽  
Human Bone ◽  
Angelica Sinensis
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