Expression of potassium channels is relevant for cell survival and migration in a murine bone marrow stromal cell line

2019 ◽  
Vol 234 (10) ◽  
pp. 18086-18097
Author(s):  
Henrique B. Silva ◽  
Deivid C. Rodrigues ◽  
Raiana Andrade ◽  
Gabriel H. G. S. F. Teixeira ◽  
Mariana P. Stelling ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Potassium Channels ◽  
Cell Survival ◽  
Cell Line ◽  
Stromal Cell ◽  
Bone Marrow Stromal Cell ◽  
Marrow Stromal Cell ◽  
Murine Bone Marrow ◽  
Stromal Cell Line ◽  
And Migration

scholarly journals Improved hematopoiesis in anemic Sl/Sld mice by splenectomy and therapeutic transplantation of a hematopoietic microenvironment

Blood ◽  
1989 ◽  
Vol 74 (3) ◽  
pp. 1144-1151
Author(s):  
P Anklesaria ◽  
TJ FitzGerald ◽  
K Kase ◽  
A Ohara ◽  
JS Greenberger
Keyword(s):  
Bone Marrow ◽  
Cell Line ◽  
Hind Limb ◽  
Stromal Cell ◽  
Bone Marrow Stromal Cell ◽  
Volume Distribution ◽  
Distribution Analysis ◽  
Marrow Stromal Cell ◽  
Stromal Cell Line ◽  
The Right

The ability of a clonal hematopoiesis-supportive bone-marrow stromal cell line GBlneor to engraft and alter the microenvironment-induced anemia of Sl/Sld mice was studied. Prior to stromal cell transplantation, Sl/Sld mice received 1 Gy total body irradiation (TBI) and 13 Gy to the right hind limb. Two months after intravenous (IV) injection of 5 x 10(5) GBlneor cells, 54.4% +/- 17.0% donor origin (G418r) colony-forming cells were recovered from the right hind limb of Sl/Sld mice. Long-term bone marrow cultures (LTBMCs) established from GBlneor-transplanted mice produced 189.5 CFU-GEMM-forming progenitors/flask over 10 weeks compared with 52.7 +/- 6.2 CFU-GEMM forming progenitors/flask from irradiated nontransplanted Sl/Sld mice. A partial correction of macrocytic anemia was detected 2 months after GBlneor transplantation in splenectomized, irradiated Sl/Sld mice (HgB 7.2 +/- 0.4 g/dL; MCV 68.3 +/- 7.0 fL) compared to splenectomized, irradiated, nontransplanted Sl/Sld mice (HgB 5.5 +/- 1.1 g/dL; MCV 76 +/- 8.5 fL) or control Sl/Sld mice (HgB 5.4 +/- 0.5 g/dL; MCV 82.4 +/- 1.3 fL). Mean RBC volume distribution analysis showed a 2.5-fold increase in percentage of peripheral blood RBCs with MCV less than or equal to 45 fL and confirmed reduction of the MCV in splenectomized- GBlneor-transplanted mice compared to control Sl/Sld mice. A hematopoiesis-suppressive clonal stromal cell line derived from LTBMCs of Sl/Sld mice (Sldneor) engrafted as effectively (43.5% +/- 1.2% G418r CFU-F/limb) as did GBlneor cells (38.3% +/- 0.16% G418r CFU-F/limb) to the irradiated right hind limbs of C57Bl/6 mice. LTBMCs established after 2 or 6 months from Sldneor-transplanted mice showed decreased hematopoiesis (182 +/- 12 [2 months] and 3494.3 +/- 408.1 [6 months] CFU-GEMM forming progenitors/flask over 10 weeks) compared to those established from GBlneor-transplanted mice (5980 +/- 530 [2 months] and 7728 +/- 607, [6 months] CFU-GEMM progenitors forming/flask). Thus, transplantation of clonal bone-marrow stromal cell lines in vivo can stably transfer their physiologic properties to normal or mutant mice.

A mouse bone marrow stromal cell line, TBR-B, shows inducible expression of smooth muscle-specific genes

FEBS Letters ◽  
2000 ◽  
Vol 481 (2) ◽  
pp. 193-196 ◽  
Author(s):  
Emi Arakawa ◽  
Kazuhide Hasegawa ◽  
Nobuaki Yanai ◽  
Masuo Obinata ◽  
Yuzuru Matsuda
Keyword(s):  
Bone Marrow ◽  
Smooth Muscle ◽  
Cell Line ◽  
Stromal Cell ◽  
Bone Marrow Stromal Cell ◽  
Inducible Expression ◽  
Mouse Bone Marrow ◽  
Marrow Stromal Cell ◽  
Stromal Cell Line

Multipotency of a Bone Marrow Stromal Cell Line, TBR31-2, Established from ts-SV40 T Antigen Gene Transgenic Mice

2000 ◽  
Vol 268 (2) ◽  
pp. 450-455 ◽  
Author(s):  
Yoichi Negishi ◽  
Akihiko Kudo ◽  
Akiko Obinata ◽  
Kohtaro Kawashima ◽  
Hiroshi Hirano ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Transgenic Mice ◽  
Cell Line ◽  
Stromal Cell ◽  
Bone Marrow Stromal Cell ◽  
T Antigen ◽  
Sv40 T Antigen ◽  
Marrow Stromal Cell ◽  
Antigen Gene ◽  
Stromal Cell Line

Two Cellular Components of Bone Marrow Origin Contribute to Pulmonary Irradiation Fibrosis.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 1401-1401
Author(s):  
Joel S. Greenberger ◽  
Yunyun Niu ◽  
Xichen Zhang ◽  
Shaonan Cao ◽  
Timothy Carlos ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Cell Line ◽  
Stromal Cell ◽  
Bone Marrow Stromal Cell ◽  
Cellular Migration ◽  
Stromal Fibroblasts ◽  
Marrow Stromal Cell ◽  
Stromal Cell Line ◽  
Lung Irradiation ◽  
Cellular Components

Abstract Following lung irradiation of C57BL/6NHsd mice, migration of marrow origin macrophages into the lungs is detectable at 100 days followed by migration of marrow origin fibroblasts into areas of developing organizing alveolitis/fibrosis. To demonstrate the importance of each cellular migration, mice were irradiated to 20 Gy to the pulmonary cavity. Beginning at day 80, the mice were injected (daily for 15 days I.P.) with antibodies to either mouse macrophages or to a pan-T-cell antigen (M1/70 or TIB-207, respectively). Groups of mice were injected at day 80 with DS-red labeled Smad3+/+ clonal bone marrow stromal cell line cells or a GFP+ Smad3−/− bone marrow stromal cell line. The mice were then followed for survival. Mice injected with the M1/70 antibody had increased survival compared to those receiving TIB-207 or control irradiated mice (percent survival at 180 days 90% and 60% compared to 40%, p=0.0225 and 0.8263). There was a decrease in the number of macrophages in the lungs of mice injected with M1/70 compared to TIB-207 injected or control irradiated mice. Mice injected with bone marrow stromal cells from the Smad3−/− line, which have abrogation of the TGF-β signaling pathway, showed decreased numbers of labeled cells in areas of organizing alveolitis/fibrosis compared to mice that were injected with Smad3+/+ cells at 120 days (5.4 ± 1.6% vs. 21.0 ± 3.3% cells per 100x high powered field in areas of organizing alveolitis/fibrosis, respectively, p = 0.0003). These results demonstrate a critical importance of both marrow hematopoietic (macrophage) and stromal fibroblasts to the development of irradiation-induced pulmonary fibrosis.

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