Myostatin induces tumor necrosis factor‐α expression in rheumatoid arthritis synovial fibroblasts through the PI3K–Akt signaling pathway

2018 ◽  
Vol 234 (6) ◽  
pp. 9793-9801 ◽  
Author(s):  
Chen‐Ming Su ◽  
Sung‐Lin Hu ◽  
Yi Sun ◽  
Jin Zhao ◽  
Chengqian Dai ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Tumor Necrosis Factor ◽  
Signaling Pathway ◽  
Tumor Necrosis ◽  
Tumor Necrosis Factor Α ◽  
Synovial Fibroblasts ◽  
Akt Signaling ◽  
Akt Signaling Pathway ◽  
Factor Α ◽  
Necrosis Factor

Inhibition of the PI3K-Akt Signaling Pathway Reduces Tumor Necrosis Factor-α Production in Response to Titanium Particles in Vitro

2007 ◽  
Vol 89 (5) ◽  
pp. 1019-1027 ◽  
Author(s):  
Matthew V. Smith ◽  
Michael J. Lee ◽  
Andrew S. Islam ◽  
Jacqueline L. Rohrer ◽  
Victor M. Goldberg ◽  
...  
Keyword(s):  
Tumor Necrosis Factor ◽  
Signaling Pathway ◽  
Tumor Necrosis ◽  
Tumor Necrosis Factor Α ◽  
Akt Signaling ◽  
Akt Signaling Pathway ◽  
Factor Α ◽  
Necrosis Factor

scholarly journals JAB1 Determines the Response of Rheumatoid Arthritis Synovial Fibroblasts to Tumor Necrosis Factor-α

2006 ◽  
Vol 169 (3) ◽  
pp. 889-902 ◽  
Author(s):  
Jianhua Wang ◽  
Chuanyu Li ◽  
Yuelong Liu ◽  
Wan Mei ◽  
Shaohua Yu ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Tumor Necrosis Factor ◽  
Tumor Necrosis ◽  
Tumor Necrosis Factor Α ◽  
Synovial Fibroblasts ◽  
Factor Α ◽  
Necrosis Factor

scholarly journals Tumor Necrosis Factor α-Induced MicroRNA-18a Activates Rheumatoid Arthritis Synovial Fibroblasts Through a Feedback Loop in NF-κB Signaling

2013 ◽  
Vol 65 (4) ◽  
pp. 916-927 ◽  
Author(s):  
Michelle Trenkmann ◽  
Matthias Brock ◽  
Renate E. Gay ◽  
Beat A. Michel ◽  
Steffen Gay ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Tumor Necrosis Factor ◽  
Tumor Necrosis ◽  
Feedback Loop ◽  
Tumor Necrosis Factor Α ◽  
Synovial Fibroblasts ◽  
Factor Α ◽  
Necrosis Factor

scholarly journals Kruppel-like factor 4 upregulates the resistance to apoptosis induced by tumor necrosis factor α in synovial fibroblasts with rheumatoid arthritis

2021 ◽  
Vol 19 ◽  
pp. 205873922110317
Author(s):  
Chenghong Ni ◽  
Shiyi Zeng ◽  
Chen Zhang ◽  
Kehan Lao ◽  
Jifeng Wang ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Cell Proliferation ◽  
Tumor Necrosis Factor ◽  
Cell Apoptosis ◽  
Tumor Necrosis ◽  
Tumor Necrosis Factor Α ◽  
Caspase 3 ◽  
Synovial Fibroblasts ◽  
Factor Α ◽  
Necrosis Factor

Objective The objective is to examine the effect of tumor necrosis factor α (TNFα) on apoptosis and proliferation of rheumatoid arthritis synovial fibroblasts (RASFs) and to elucidate the regulatory roles of Kruppel-like factor 4 (KLF4) in TNFα-induced RASF apoptosis. Methods Changes in cell proliferation were measured using an 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di- phenytetrazoliumromide (MTT) assay, and changes in cell apoptosis were detected by flow cytometry and Hoechst 33258 staining. Changes in the apoptosis-related protein caspase-3 and the apoptosis-related genes bcl-2/bax were measured by western blot and real-time PCR, respectively Results TNFα stimulation increased cell proliferation ( p < 0.05), decreased cell apoptosis ( p < 0.05), declined caspase-3 expression ( p < 0.05), and upregulated bcl-2/bax level ( p < 0.05) in RASFs. KLF4 gene silencing decreased cell proliferation ( p < 0.05), increased cell apoptosis ( p < 0.05), upregulated caspase-3 expression ( p < 0.05), and downregulated bcl-2/bax level ( p < 0.05) induced by TNFα in RASFs. Conclusions TNFα caused a decrease in RASF apoptosis, and KLF4 promoted resistance to TNFα-induced apoptosis and cell proliferation.

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