High fat diet reduces the expression of miRNA‐29b in heart and increases susceptibility of myocardium to ischemia/reperfusion injury

2018 ◽  
Vol 234 (6) ◽  
pp. 9399-9407 ◽  
Author(s):  
Elaine Castilho Guedes ◽  
Ivson Bezerra da Silva ◽  
Vanessa Morais Lima ◽  
Juliane B. Miranda ◽  
Rudá P. Albuquerque ◽  
...  
Keyword(s):  
Reperfusion Injury ◽  
High Fat Diet ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
High Fat

scholarly journals Comparative evaluation of metformin and liraglutide cardioprotective effect in rats with impaired glucose tolerance

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Anna Simanenkova ◽  
Sarkis Minasian ◽  
Tatiana Karonova ◽  
Timur Vlasov ◽  
Natalya Timkina ◽  
...  
Keyword(s):  
Glucose Tolerance ◽  
Impaired Glucose Tolerance ◽  
Reperfusion Injury ◽  
High Fat Diet ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Control Group ◽  
Hemodynamic Parameters ◽  
High Fat ◽  
Once Daily

AbstractImpaired glucose tolerance (IGT) increases cardiovascular risk and can enlarge myocardial infarction (MI) incidence and severity. There is lack of information about cardioprotective potential of glucose-lowering drugs in IGT. We aimed to evaluate the sustainability of myocardium to ischemia–reperfusion injury in diabetic and IGT rats and to study cardioprotective action of metformin and liraglutide. Type 2 diabetes mellitus (DM) and IGT were modelled in Wistar rats by high-fat diet and streptozotocin + nicotinamide. 4 weeks after rats were divided into 4 groups: DM (without treatment) (n = 4), IGT (without treatment) (n = 4), IGT + MET (metformin 200 mg/kg per os once daily 8 weeks) (n = 4), IGT + LIRA (liraglutide 0.06 mg/kg s.c. once daily for 8 weeks) (n = 4). Control (n = 6) and high-fat diet (n = 8) groups were made for comparison. After 8 weeks ischemia–reperfusion injury in isolated hearts was performed. Hemodynamic parameters were evaluated and MI size was measured by staining of myocardium slices in triphenyltetrazolium chloride solution. Blood glucose level was measured during the study. Both IGT and DM led to similar worsening of hemodynamic parameters during ischemia–reperfusion period, in comparison with control group. MI size in IGT (56.76 (51.58; 69.07) %) and DM (57.26 (45.51; 70.08) %) groups was significantly larger than that in control group (42.98 (33.26; 61.84) %) and did not differ between each other. MI size in high-fat diet group (56.98 (47.11; 62.83) %) was as large as in IGT and DM groups (p > 0.05). MI size in IGT + MET (42.11 (38.08; 71.96) %) and IGT + LIRA (42.50 (31.37; 60.40) %) was smaller than in both DM and IGT groups (p < 0.05 for multiple comparison). Myocardium damage size did not differ in IGT + MET and IGT + LIRA groups (p >  0.05). Only LIRA, but not MET administration to IGT rats led to ischemic contracture reduction. Glycemic control was similarly satisfactory in IGT, IGT + MET, IGT + LIRA groups. Thus, IGT and DM have similarly pronounced negative influence on hemodynamics and MI size in rat transient global ischemia ex vivo. Obesity development also has negative impact on the MI size. Both MET and LIRA have infarct-limiting effect independent on their influence on glucose level. LIRA, but not MET, diminishes ischemic contracture in IGT rats.

scholarly journals Comparative Evaluation of Metformin and Liraglutide Cardioprotective Effect in Rats With Impaired Glucose Tolerance

2020 ◽  
Author(s):  
Anna Simanenkova ◽  
Sarkis Minasian ◽  
Tatiana Karonova ◽  
Timur Vlasov ◽  
Natalya Timkina ◽  
...  
Keyword(s):  
Glucose Tolerance ◽  
Impaired Glucose Tolerance ◽  
Reperfusion Injury ◽  
High Fat Diet ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Control Group ◽  
Hemodynamic Parameters ◽  
High Fat ◽  
Once Daily

Abstract Background: Impaired glucose tolerance (IGT) increases cardiovascular risk and can enlarge myocardial infarction (MI) incidence and severity. There is lack of information about cardioprotective potential of glucose-lowering drugs in IGT. We aimed to evaluate the sustainability of myocardium to ischemia-reperfusion injury in diabetic and IGT rats and to study cardioprotective action of metformin and liraglutide. Methods: Type 2 diabetes mellitus (DM) and IGT were modelled in Wistar rats by high-fat diet and streptozotocin+nicotinamide. 4 weeks after rats were divided into 4 groups: DM (without treatment), IGT (without treatment), IGT+MET (metformin 200 mg/kg per os once daily 8 weeks), IGT+LIRA (liraglutide 0.06 mg/kg s.c. once daily for 8 weeks). Control and high-fat diet groups were made for comparison. After 8 weeks ischemia-reperfusion injury of isolated hearts was performed. Hemodynamic parameters were evaluated and MI size was measured by staining of myocardium slices in triphenyltetrazolium chloride solution. Blood glucose level was measured during the study.Results: Both IGT and DM led to similar worsening of hemodynamic parameters during ischemia-reperfusion period, in comparison with control group. MI size in IGT (56.76 (51.58; 69.07) %) and DM (57.26 (45.51; 70.08) %) groups was significantly large than in control group (42.98 (33.26; 61.84) %) and did not differ between each other. MI size in high-diet group (56.98 (47.11; 62.83) %) was similarly large as in IGT and DM groups (p>0.05). MI size in IGT+MET (42.11 (38.08; 71.96) %) and IGT+LIRA (42.50 (31.37; 60.40) %) was smaller than in both DM and IGT groups (p<0.05 for multiple comparison). Myocardium damage size did not differ in IGT+MET and IGT+LIRA groups (p>0.05). Only LIRA, but not MET administration to IGT rats led to ischemic contracture reduction. Glycemic control was similar satisfactory in IGT, IGT+MET, IGT+LIRA groups. Conclusions: IGT and DM have similarly pronounced negative influence on hemodynamics and MI size in transient global rat ischemia ex vivo. Obesity development also has negative impact on the myocardial infarct size. Both MET and LIRA have infarct-limiting effect independent on their influence on glucose level. LIRA, but not MET, diminishes ischemic contracture in IGT rats.

scholarly journals Male and Female Rats Have Different Physiological Response to High-Fat High-Sucrose Diet but Similar Myocardial Sensitivity to Ischemia-Reperfusion Injury

Nutrients ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 2914
Author(s):  
Natacha Fourny ◽  
Carole Lan ◽  
Monique Bernard ◽  
Martine Desrois
Keyword(s):  
Reperfusion Injury ◽  
Glucose Intolerance ◽  
Physiological Response ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
High Fat ◽  
Male And Female ◽  
Sucrose Diet ◽  
High Sucrose Diet ◽  
High Sucrose

Prediabetes is a strong predictor of type 2 diabetes and its associated cardiovascular complications, but few studies explore sexual dimorphism in this context. Here, we aim to determine whether sex influences physiological response to high-fat high-sucrose diet (HFS) and myocardial tolerance to ischemia-reperfusion injury. Male and female Wistar rats were subjected to standard (CTRL) or HFS diet for 5 months. Then, ex-vivo experiments on isolated perfused heart model were performed to evaluate tolerance to ischemia-reperfusion injury. HFS diet induced fasting hyperglycemia and increased body fat percent to a similar level in both sexes. However, glucose intolerance was more pronounced in female HFS. Cholesterol was increased only in female while male displayed higher level of plasmatic leptin. We observed increased heart weight to tibia length ratio only in males, but we showed a similar decrease in tolerance to ischemia-reperfusion injury in female and male HFS compared with respective controls, characterized by impaired cardiac function, energy metabolism and coronary flow during reperfusion. In conclusion, as soon as glucose intolerance and hyperglycemia develop, we observe higher sensitivity of hearts to ischemia-reperfusion injury without difference between males and females.

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