Antibodies reacting to mimotopes of Simian virus 40 large T antigen, the viral oncoprotein, in sera from children

2018 ◽  
Vol 234 (4) ◽  
pp. 3170-3179 ◽  
Author(s):  
Elisa Mazzoni ◽  
Francesca Frontini ◽  
John Charles Rotondo ◽  
Nunzia Zanotta ◽  
Arianna Fioravanti ◽  
...  
Keyword(s):  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
Large T Antigen ◽  
Viral Oncoprotein

scholarly journals 80DEVELOPMENTAL POTENTIAL OF BOVINE NUCLEAR TRANSFER EMBRYOS DERIVED FROM FETAL FIBROBLASTS TRANSDUCED WITH SIMIAN VIRUS 40 LARGE T ANTIGEN

2004 ◽  
Vol 16 (2) ◽  
pp. 162
Author(s):  
V. Zakhartchenko ◽  
F. Yang ◽  
B. Vogg ◽  
A. Pfeifer ◽  
E. Wolf
Keyword(s):  
Nuclear Transfer ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
Proliferative Potential ◽  
Large T Antigen ◽  
Viral Oncoprotein ◽  
Developmental Potential ◽  
Fetal Fibroblasts ◽  
Gfp Tag

Primary cultured cells with limited lifespan are generally used as donors for nuclear transfer (NT). Immortalization or even extension of their proliferative capacity would provide an additional time-window for various genetic manipulations prior to NT. Previously, we reported that a spontaneously immortalized mammary epithelial cell line failed to support development of reconstructed embryos into blastocysts [Zakhartchenko et al., 1999 Mol. Reprod. Dev. 54, 264–272]. In contrast, telomerase-immortalized sheep fibroblasts could be substantially reprogrammed, although they were not fully competent for NT since no fetuses survived beyond 40 days of development [Cui et al., 2003 Biol. Reprod. 69, 15–21]. Simian virus 40 large T antigen (SV40Tag), a viral oncoprotein, is known to immortalize human diploid fibroblasts by soaking up the cellular negative growth regulators, pRb, p53, and some related factors to enable cells to grow continuously [Kim et al., 2001 Exp. Mol. Med. 33, 293–298]. In this study we examined the developmental competence of bovine NT embryos derived from SV40Tag-transduced fetal fibroblasts. Primary fetal fibroblasts (BFF) obtained from a 49-day-old fetus were first transduced with the green fluorescent protein (GFP) gene and then with SV40Tag using a replication-defective retrovirus. GFP-SV40Tag-positive cells (BFF-GFP-Tag), which can proliferate over 50 passages, were cultured until confluence and then used for nuclear transfer at passages 27–30. As control, confluent GFP-positive cells (BFF-GFP) and BFF, which stopped proliferation after 28 passages, were used at passages 4–6 and 1–2, respectively. Nuclear transfer and embryo culture procedures were essentially as described previously [Shi et al., 2003 Biol. Reprod. 69, 301–309]. Data were compared using chi-square test; differences were considered significant for P<0.05. There were no significant differences in the fusion and cleavage rates between all three groups [BFF-GFP-Tag: 233/248 (94%) and 167/233 (72%); BFF-GFP: 274/294 (93%) and 216/274 (79%); BFF: 129/136 (96%) and 97/129 (75%)]. However, development to the blastocyst stage was significantly lower (P<0.05) in the BFF-GFP-Tag group [5/233 (2%)] than in the BFF-GFP and BFF groups [110/275 (40%) and 53/129 (41%), respectively)]. From our results we conclude that SV40Tag-transduced bovine fetal fibroblasts with high proliferative potential may have lost the ability to respond to the normal cell cycle controls and, as a consequence, have low developmental potential for nuclear transfer.

scholarly journals Serum IgG Antibodies from Pregnant Women Reacting to Mimotopes of Simian Virus 40 Large T Antigen, the Viral Oncoprotein

2017 ◽  
Vol 8 ◽  
Author(s):  
Elisa Mazzoni ◽  
Mariantonietta Di Stefano ◽  
Josè R. Fiore ◽  
Federica Destro ◽  
Marco Manfrini ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
Large T Antigen ◽  
Igg Antibodies ◽  
Viral Oncoprotein ◽  
Serum Igg

scholarly journals Serum Antibodies Against Simian Virus 40 Large T Antigen, the Viral Oncoprotein, in Osteosarcoma Patients

2018 ◽  
Vol 6 ◽  
Author(s):  
Elisa Mazzoni ◽  
Ilaria Bononi ◽  
Maria S. Benassi ◽  
Piero Picci ◽  
Elena Torreggiani ◽  
...  
Keyword(s):  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
Large T Antigen ◽  
Serum Antibodies ◽  
Viral Oncoprotein

scholarly journals Cul7/p185/p193 Binding to Simian Virus 40 Large T Antigen Has a Role in Cellular Transformation

2004 ◽  
Vol 78 (6) ◽  
pp. 2749-2757 ◽  
Author(s):  
Syed Hamid Ali ◽  
Jocelyn S. Kasper ◽  
Takehiro Arai ◽  
James A. DeCaprio
Keyword(s):  
Growth Control ◽  
Simian Virus 40 ◽  
Cellular Transformation ◽  
Soft Agar ◽  
Simian Virus ◽  
T Antigen ◽  
Large T Antigen ◽  
Independent Manner ◽  
Viral Oncoprotein ◽  
Primary Mouse

ABSTRACT Simian virus 40 large T antigen (TAg) is a viral oncoprotein that can promote cellular transformation. TAg's transforming activity results in part by binding and inactivating key tumor suppressors, including p53 and the retinoblastoma protein (pRb). We have identified a TAg-associated 185-kDa protein that has significant homology to the cullin family of E3 ubiquitin ligases. TAg binds to an SCF-like complex that contains p185/Cul7, Rbx1, and the F box protein Fbw6. This SCF-like complex binds to an N-terminal region of TAg. Several p185/Cul7-binding-deficient mutants of TAg were generated that retained binding to pRb and p53 and were capable of overcoming Rb-mediated repression of E2F transcription. Despite binding to pRb and p53, these p185/Cul7-binding-defective mutants of TAg were unable to transform primary mouse embryo fibroblasts. Cells expressing p185/Cul7-binding-defective mutants of TAg were unable to grow to high density or grow in an anchorage-independent manner as determined by growth in soft agar. Considering the significance of other TAg-interacting proteins in regulation of the cell cycle, p185/Cul7 may also regulate an important growth control pathway.

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