MicroRNA‐212 promotes the recovery function and vascular regeneration of endothelial progenitor cells in mice with ischemic stroke through inactivation of the notch signaling pathway via downregulating MMP9 expression

2018 ◽  
Author(s):  
Chen Hu ◽  
Zhi‐Ling Dong
Keyword(s):  
Ischemic Stroke ◽  
Notch Signaling ◽  
Progenitor Cells ◽  
Signaling Pathway ◽  
Endothelial Progenitor Cells ◽  
Notch Signaling Pathway ◽  
Endothelial Progenitor ◽  
Recovery Function ◽  
Vascular Regeneration ◽  
Mmp9 Expression

Abstract 1941: Level and Value of Circulating Endothelial Progenitor Cells in Patients after Acute Ischemic Stroke

Circulation ◽  
2007 ◽  
Vol 116 (suppl_16) ◽  
Author(s):  
Yip Hon-Kan
Keyword(s):  
At Risk ◽  
Ischemic Stroke ◽  
Acute Ischemic Stroke ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Neurological Impairment ◽  
Systemic Circulation ◽  
Endothelial Progenitor ◽  
Tissue Ischemia ◽  
Severe Neurological Impairment

Background and Purpose Endothelial progenitor cells (EPCs) migrate from bone marrow to systemic circulation in response to tissue ischemia where they differentiate into mature endothelial cells for in situ angiogenesis. This study tested the hypothesis that the level of circulating EPCs is substantially increased and predictive of prognostic outcomes after acute ischemic stroke. Methods The level of circulating EPCs [staining markers: CD31/CD34 (E 1 ), CD62E/CD34 (E 2 ) and KDR/CD34 (E 3 )] was examined using flow cytometry at 48 h after acute ischemic stroke in 138 consecutive patients. The EPC level was also evaluated once in twenty healthy volunteers and in forty at-risk controls. Results Level of circulating EPCs (E 1–3 ) was significantly higher in ischemic stroke patients than in at-risk control subjects ( p <0.05). Additionally, EPC (E 1–3 ) level was significantly lower in patients with severe neurological impairment [defined as a score ≥12 on the National Institute of Health Stroke Scale (NIHSS)] than in patients with less severe impairment (NIHSS < score 12) at 48 h after ischemic stroke ( p <0.0001). Moreover, the EPC (E 3 ) level was strongly correlated with improved NIHSS ≥ 4 on day 21 after ischemic stroke ( p =0.0004). Furthermore, low circulating EPC level was independently predictive of severe neurological impairment (NIHSS ≥ 12) at 48 h (E 1–3 ) and combined major adverse clinical outcomes (defined as recurrent ischemic stroke, any cause of death, or NIHSS of ≥ 12) on day 90 (E 1 ) following ischemic stroke ( p <0.001). Conclusions Level of circulating EPCs is independently predictive of prognosis after ischemic stroke.

Intra-carotid arterial transfusion of circulatory-derived autologous endothelial progenitor cells in rodent after ischemic stroke — evaluating the impact of therapeutic time points on prognostic outcomes

2020 ◽  
Author(s):  
Kun-Chen Lin ◽  
Han-Tan Chai ◽  
Kuan-Hung Chen ◽  
Pei‐Hsun Sung ◽  
John Y. Chiang ◽  
...  
Keyword(s):  
Ischemic Stroke ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Optimal Time ◽  
Control Group ◽  
Endothelial Progenitor ◽  
Group 2 ◽  
The Impact ◽  
Carotid Arterial ◽  
Group 1

Abstract Background: This study tested the optimal time point for left intra-carotid arterial (LICA) administration of circulatory-derived autologous endothelial progenitor cells (EPCs) for improving the outcome in rat after acute ischemic stroke (IS). Methods and Results: Adult-male SD rats (n=70) were equally categorized into group 1 (sham-operated control), group 2 (IS), group 3 (IS+EPCs/1.2x106 cells/by LICA administration 3h after IS), group 4 (IS+EPCs/LICA administration post-day-3 IS), group 5 (IS+EPCs/LICA administration post-day-7 IS), group 6 (IS+EPCs/LICA administration post-day-14 IS) and group 7 (IS+EPCs/LICA administration post-day-28 IS). The brain-infarct volume (BIV) (at day 60/MRI) was lowest in group 1, highest in group 2 and significantly progressively increased from groups 3 to 7, whereas among the IS animals, the neurological function was significantly preserved in groups 3 to 6 than in groups 2 and 7 post-day-60 IS (all p<0.0001). By day 60, the endothelial cell markers at protein and cellular levels, and number of small vessels exhibited an opposite pattern of BIV among the groups (all p<0.0001). The protein and cellular levels of inflammation, and protein levels of oxidative stress, autophagy and apoptosis, were highest in group 2, lowest in group 1 and progressively increased from groups 3 to 7 (all p<0.0001). The angiogenesis biomarkers at protein and cellular levels were significantly progressively increased from groups 1 to 3, then significantly progressively decreased from groups 4 to 7 (all p<0.0001). Conclusion: Early EPC administration provided better benefits on improving functional/image/molecular-cellular outcomes after acute IS in rat.

Potential implications of vascular wall resident endothelial progenitor cells

2007 ◽  
Vol 98 (11) ◽  
pp. 930-939 ◽  
Author(s):  
Derya Tilki ◽  
Hans-Peter Hohn ◽  
Ursula Gehling ◽  
Nerbil Kilic ◽  
Süleyman Ergün
Keyword(s):  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Peripheral Blood ◽  
Tumor Therapy ◽  
Vascular Wall ◽  
Endothelial Progenitor ◽  
Vascular Regeneration ◽  
Predictive Role ◽  
Vascular Formation

SummaryA rapidly increasing body of data suggests an essential role of endothelial progenitor cells (EPCs) in vascular regeneration, formation of new vessels in cardiovascular diseases and also in tumor vasculogenesis. Moreover, recent data obtained from clinical studies with anti-angiogenic drugs in tumor therapy or with pro-angiogenic stimuli in ischemic disorders implicate a predictive role of the number of EPCs circulating in the peripheral blood in monitoring of these diseases. However, there is still some controversial data regarding the relevance of the EPCs in vascular formation depending on models used and diseases studied. One of the essential prerequisites for a better understanding of the whole contribution of EPCs to vascular formation in adult, a process called postnatal vasculogenesis, is to identify their exact sources. We could recently discover the existence of EPCs in a distinct zone of the vascular wall of large and middle sized adult blood vessels and showed that these cells are capable to differentiate into mature endothelial cells, to form capillary sprouts in arterial ring assay and to build vasa vasorumlike structures within the vascular wall. They also can be mobilized very rapidly from the vascular wall by tumor cells. This review will discuss the functional implications of these vascular wall resident endothelial progenitor cells (VW-EPCs) in relation to those of EPCs circulating in peripheral blood or derived from the bone marrow in cardiovascular and neoplastic diseases.

scholarly journals Vascular Regeneration by Endothelial Progenitor Cells in Health and Diseases

10.5772/64529 ◽  
2016 ◽  
Author(s):  
Estefanía Nova-Lamperti ◽  
Felipe Zúñiga ◽  
Valeska Ormazábal ◽  
Carlos Escudero ◽  
Claudio Aguayo
Keyword(s):  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Endothelial Progenitor ◽  
Vascular Regeneration

scholarly journals The angiogenic gene profile of circulating endothelial progenitor cells from ischemic stroke patients

Vascular Cell ◽  
2013 ◽  
Vol 5 (1) ◽  
pp. 3 ◽  
Author(s):  
Míriam Navarro-Sobrino ◽  
Mar Hernández-Guillamon ◽  
Israel Fernandez-Cadenas ◽  
Marc Ribó ◽  
Ignacio A Romero ◽  
...  
Keyword(s):  
Ischemic Stroke ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Gene Profile ◽  
Stroke Patients ◽  
Endothelial Progenitor ◽  
Circulating Endothelial Progenitor Cells ◽  
Angiogenic Gene
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