Deglycosylation of epithelial cell adhesion molecule affects epithelial to mesenchymal transition in breast cancer cells

2018 ◽  
Vol 234 (4) ◽  
pp. 4504-4514 ◽  
Author(s):  
Xue Liu ◽  
Liu Yang ◽  
Dandan Zhang ◽  
Tingjiao Liu ◽  
Qiu Yan ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Adhesion ◽  
Epithelial Cell ◽  
Cancer Cells ◽  
Adhesion Molecule ◽  
Breast Cancer Cells ◽  
Cell Adhesion Molecule ◽  
Epithelial To Mesenchymal Transition ◽  
Epithelial Cell Adhesion Molecule ◽  
Mesenchymal Transition

scholarly journals The role of epithelial cell adhesion molecule N-glycosylation on apoptosis in breast cancer cells

Tumor Biology ◽  
2017 ◽  
Vol 39 (3) ◽  
pp. 101042831769597 ◽  
Author(s):  
Dandan Zhang ◽  
Xue Liu ◽  
Jiujiao Gao ◽  
Yan Sun ◽  
Tingjiao Liu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Proliferation ◽  
Cell Adhesion ◽  
Epithelial Cell ◽  
Cancer Cells ◽  
Adhesion Molecule ◽  
Breast Cancer Cells ◽  
Cell Adhesion Molecule ◽  
Mitogen Activated Protein Kinase ◽  
Epithelial Cell Adhesion Molecule

Glycosylation of cell surface proteins plays an important role in the regulation of apoptosis. It has been demonstrated that knockdown of epithelial cell adhesion molecule promoted apoptosis, inhibited cell proliferation, and caused cell-cycle arrest. In this study, we investigated whether and how N-glycosylation of epithelial cell adhesion molecule influenced the apoptosis in breast cancer cells. We applied the N-glycosylation mutation epithelial cell adhesion molecule plasmid to express deglycosylation of epithelial cell adhesion molecule and then to study its function. Our results showed that deglycosylation of epithelial cell adhesion molecule promoted apoptosis and inhibited cell proliferation. Deglycosylation of epithelial cell adhesion molecule enhanced the cytotoxic effect of 5-fluorouracil, promoting apoptosis by downregulating the expression of the anti-apoptotic protein Bcl-2 and upregulating the expression of the pro-apoptotic proteins Bax and Caspase 3 via the extracellular-signal-regulated kinase 1/2 and c-Jun N-terminal kinase mitogen-activated protein kinase signaling pathways in MCF-7 and MDA-MB-231 cells. These findings are important for a better understanding of epithelial cell adhesion molecule apoptosis regulation and suggest epithelial cell adhesion molecule as a potential target for the treatment of breast cancer.

scholarly journals The association between Annexin A2 and epithelial cell adhesion molecule in breast cancer cells

2021 ◽  
Author(s):  
Saad Misfer Al‐Qahtani ◽  
Salah Eldin Gadalla ◽  
Min Guo ◽  
Christer Ericsson ◽  
Daniel Hägerstrand ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Adhesion ◽  
Epithelial Cell ◽  
Cancer Cells ◽  
Adhesion Molecule ◽  
Breast Cancer Cells ◽  
Cell Adhesion Molecule ◽  
Annexin A2 ◽  
Epithelial Cell Adhesion Molecule

scholarly journals Epithelial Cell Adhesion Molecule: An Anchor to Isolate Clinically Relevant Circulating Tumor Cells

Cells ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 1836
Author(s):  
Zahra Eslami-S ◽  
Luis Enrique Cortés-Hernández ◽  
Catherine Alix-Panabières
Keyword(s):  
Cell Adhesion ◽  
Epithelial Cell ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Epithelial To Mesenchymal Transition ◽  
Epithelial Cell Adhesion Molecule ◽  
Mesenchymal Transition ◽  
Advantages And Disadvantages

In the last few decades, the epithelial cell adhesion molecule (EpCAM) has received increased attention as the main membrane marker used in many enrichment technologies to isolate circulating tumor cells (CTCs). Although there has been a great deal of progress in the implementation of EpCAM-based CTC detection technologies in medical settings, several issues continue to limit their clinical utility. The biology of EpCAM and its role are not completely understood but evidence suggests that the expression of this epithelial cell-surface protein is crucial for metastasis-competent CTCs and may not be lost completely during the epithelial-to-mesenchymal transition. In this review, we summarize the most significant advantages and disadvantages of using EpCAM as a marker for CTC enrichment and its potential biological role in the metastatic cascade.

scholarly journals Epithelial cell adhesion molecule (EpCAM) is overexpressed in breast cancer metastases

2009 ◽  
Vol 123 (3) ◽  
pp. 701-708 ◽  
Author(s):  
Ashley Cimino ◽  
Marc Halushka ◽  
Peter Illei ◽  
Xinyan Wu ◽  
Saraswati Sukumar ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Adhesion ◽  
Epithelial Cell ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Epithelial Cell Adhesion Molecule ◽  
Breast Cancer Metastases ◽  
Cancer Metastases

scholarly journals Pivotal role of epithelial cell adhesion molecule in the survival of lung cancer cells

2011 ◽  
Vol 102 (8) ◽  
pp. 1493-1500 ◽  
Author(s):  
Tetsunari Hase ◽  
Mitsuo Sato ◽  
Kenya Yoshida ◽  
Luc Girard ◽  
Yoshihiro Takeyama ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cell Adhesion ◽  
Epithelial Cell ◽  
Cancer Cells ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Pivotal Role ◽  
Lung Cancer Cells ◽  
Epithelial Cell Adhesion Molecule
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