Regulation effects of melatonin on bone marrow mesenchymal stem cell differentiation

2018 ◽  
Vol 234 (2) ◽  
pp. 1008-1015 ◽  
Author(s):  
Biao Wang ◽  
Hao Wen ◽  
Wanli Smith ◽  
Dingjun Hao ◽  
Baorong He ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Cell Differentiation ◽  
Mesenchymal Stem Cell ◽  
Stem Cell Differentiation ◽  
Mesenchymal Stem Cell Differentiation

scholarly journals Ginsenoside Rg1 Improves Differentiation by Inhibiting Senescence of Human Bone Marrow Mesenchymal Stem Cell via GSK-3β and β-Catenin

2020 ◽  
Vol 2020 ◽  
pp. 1-16
Author(s):  
Ziling Wang ◽  
Rong Jiang ◽  
Lu Wang ◽  
Xiongbin Chen ◽  
Yue Xiang ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Cell Differentiation ◽  
Mesenchymal Stem Cell ◽  
Western Blot ◽  
Stem Cell Differentiation ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Ginsenoside Rg1 ◽  
Mesenchymal Stem Cell Differentiation

Objectives. To demonstrate the effect of Ginsenoside Rg1 on the differentiation of human bone marrow-derived mesenchymal stem cells (hBM-MSCs). Subsequently, a rational mechanism for the detection of Rg1 which affects mesenchymal stem cell differentiation was explored. Methods. Flow cytometry is used for cell identification. The differentiation ability of hBM-MSCs was studied by differentiation culture. SA-β-gal staining is used to detect cell senescence levels. Western blot and immunofluorescence were used to determine protein expression levels. RT-qPCR is used to detect mRNA expression levels. Results. Rg1 regulates the differentiation of hBM-MSCs. Differentiation culture analysis showed that Rg1 promoted cells to osteogenesis and chondrogenesis. Western blot results showed that Rg1 regulated the overactivation of the β-catenin signaling pathway and significantly adjusted the phosphorylation of GSK-3β. GSK-3β inhibitor (Licl) significantly increased Rg1-induced phosphorylation of GSK-3β, which in turn reduced Rg1-induced differentiation of hBM-MSCs. Conclusion. Ginsenoside Rg1 can reduce the excessive activation of the Wnt pathway in senescent cells by inhibiting the phosphorylation of GSK-3β and regulate the mesenchymal stem cell differentiation ability.

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