Mesenchymal stromal cells from amniotic fluid are less prone to senescence compared to those obtained from bone marrow: An in vitro study

2018 ◽  
Vol 233 (11) ◽  
pp. 8996-9006 ◽  
Author(s):  
Nicola Alessio ◽  
Caterina Pipino ◽  
Domitilla Mandatori ◽  
Pamela Di Tomo ◽  
Angela Ferone ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Amniotic Fluid ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
In Vitro Study ◽  
Vitro Study

In Vitro Study of a Novel Oxysterol for Osteogenic Differentiation on Rabbit Bone Marrow Stromal Cells

2013 ◽  
Vol 132 (1) ◽  
pp. 70e-80e ◽  
Author(s):  
Akishige Hokugo ◽  
Sarah Sorice ◽  
Anisa Yalom ◽  
James C. Lee ◽  
Andrew Li ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Osteogenic Differentiation ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
In Vitro Study ◽  
Marrow Stromal Cells ◽  
Vitro Study ◽  
Rabbit Bone

scholarly journals Clumping and Viability of Bone Marrow Derived Mesenchymal Stromal Cells under Different Preparation Procedures: A Flow Cytometry-Based In Vitro Study

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Li-li Cui ◽  
Tuure Kinnunen ◽  
Johannes Boltze ◽  
Johanna Nystedt ◽  
Jukka Jolkkonen
Keyword(s):  
Bone Marrow ◽  
Flow Cytometry ◽  
Mesenchymal Stromal Cells ◽  
Normal Saline ◽  
Stromal Cells ◽  
Pulse Width ◽  
In Vitro Study ◽  
High Viability ◽  
Storage Solutions

Complications of microocclusions have been reported after intra-arterial delivery of mesenchymal stromal cells. Hence, quantification and efficient limitation of cell clumps in suspension before transplantation is important to reduce the risk. We used a flow cytometry-based pulse-width assay to assess the effects of different cell suspension concentrations (0.2–2.0 × 106/mL), storage solutions (complete growth medium, Dulbecco’s phosphate-buffered saline, and normal saline), storage time in suspension (0–9 h), and freeze-thawing procedure on the clumping of rat bone marrow derived mesenchymal stromal cells (BMMSCs) and also evaluated cell viability at the same time. Surprisingly, increasing the cell concentration did not result in more cell clumps in vitro. Freshly harvested (fresh) cells in normal saline had significantly fewer cell clumps and also displayed high viability (>90%). A time-dependent reduction in viability was observed for cells in all three storage solutions, without any significant change in the clumping tendency except for cells in medium. Fresh cells were more viable than their frozen-thawed counterparts, and fresh cells in normal saline had fewer cell clumps. In conclusion, cell clumping and viability could be affected by different cell preparation procedures, and quantification of cell clumping can be conducted using the flow cytometry-based pulse-width assay before intra-arterial cell delivery.

scholarly journals Meniscus regeneration combining meniscus and mesenchymal stromal cells in a degradable meniscus implant: an in vitro study

2019 ◽  
Vol 38 ◽  
pp. 51-62 ◽  
Author(s):  
MH Hagmeijer ◽  
◽  
LA Vonk ◽  
M Fenu ◽  
YWAM van Keep ◽  
...  
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
In Vitro Study ◽  
Vitro Study ◽  
Meniscus Regeneration

scholarly journals Modulation properties of factors released by bone marrow stromal cells on activated microglia: an in vitro study

2014 ◽  
Vol 4 (1) ◽  
Author(s):  
Dasa Cizkova ◽  
Stéphanie Devaux ◽  
Françoise Le Marrec-Croq ◽  
Julien Franck ◽  
Lucia Slovinska ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
In Vitro Study ◽  
Marrow Stromal Cells ◽  
Vitro Study ◽  
Activated Microglia
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