Preclinical three-dimensional colorectal cancer model: The next generation of in vitro drug efficacy evaluation

2018 ◽  
Vol 234 (1) ◽  
pp. 181-191 ◽  
Author(s):  
Francesca Sensi ◽  
Edoardo D’Angelo ◽  
Sara D’Aronco ◽  
Roberto Molinaro ◽  
Marco Agostini
Keyword(s):  
Colorectal Cancer ◽  
Three Dimensional ◽  
Drug Efficacy ◽  
Next Generation ◽  
Cancer Model ◽  
Efficacy Evaluation

scholarly journals A novel tissue engineered three-dimensional in vitro colorectal cancer model

2013 ◽  
Vol 9 (8) ◽  
pp. 7917-7926 ◽  
Author(s):  
Agata Nyga ◽  
Marilena Loizidou ◽  
Mark Emberton ◽  
Umber Cheema
Keyword(s):  
Colorectal Cancer ◽  
Three Dimensional ◽  
Cancer Model

scholarly journals Beyond 3D culture models of cancer

2015 ◽  
Vol 7 (283) ◽  
pp. 283ps9-283ps9 ◽  
Author(s):  
Kandice Tanner ◽  
Michael M. Gottesman
Keyword(s):  
In Vitro Culture ◽  
Three Dimensional ◽  
3D Culture ◽  
Drug Efficacy ◽  
Spatiotemporal Evolution ◽  
Culture Models ◽  
Dynamic Interplay

The mechanisms underlying the spatiotemporal evolution of tumor ecosystems present a challenge in evaluating drug efficacy. In this Perspective, we address the use of three-dimensional in vitro culture models to delineate the dynamic interplay between the tumor and the host microenvironment in an effort to attain realistic platforms for assessing pharmaceutical efficacy in patients.

scholarly journals Next Generation Lipophilic Bisphosphonate Shows Antitumor Effect in Colorectal Cancer In Vitro and In Vivo

2020 ◽  
Vol 26 (3) ◽  
pp. 1957-1969 ◽  
Author(s):  
Marcell Baranyi ◽  
Dominika Rittler ◽  
Eszter Molnár ◽  
Senji Shirasawa ◽  
István Jalsovszky ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Antitumor Effect ◽  
Next Generation

scholarly journals The efficacy of cetuximab in a tissue-engineered three-dimensional in vitro model of colorectal cancer

2014 ◽  
Vol 5 ◽  
pp. 204173141454418 ◽  
Author(s):  
Tarig Magdeldin ◽  
Víctor López-Dávila ◽  
Cecile Villemant ◽  
Grant Cameron ◽  
Rosemary Drake ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
In Vitro Model ◽  
Three Dimensional ◽  
Vitro Model

scholarly journals Epidemiology of Sheep Lice and Efficacy Evaluation of 60% Diazinon and 1% Ivermectin against Bovicola ovis in Sayint District, South Wollo, Ethiopia

2020 ◽  
Author(s):  
Simegn Legesse ◽  
Mussie Hailemelekot ◽  
Habtamu Tamrat ◽  
Yeshwas Ferede
Keyword(s):  
Drug Efficacy ◽  
In Vitro Test ◽  
Efficacy Evaluation ◽  
Cross Sectional ◽  
Randomized Design ◽  
Present Study Area ◽  
Multistage Sampling ◽  
Associated Risk Factors

Abstract Background Sheep lice, caused by Bovicola ovis are very common in Ethiopia. It can result decreased production and reproduction, downgrading and rejection of skins in tannery industries. A cross-sectional and experimental study were conducted in Sayint District, South Wollo, Ethiopia which aims to determine the prevalence of sheep lice with the associated risk factors, identify the major sheep lice species and evaluate the efficacy of 60% Diazinon and 1% Ivermectin against Bovicola ovis. Study kebeles and animals were selected using purposive and multistage sampling techniques. Accordingly, a total of 232 sheep were sampled to estimate the prevalence of sheep lice. Fifteen sheep for in-vivo and 80 Bovicola ovis lice for in-vitro test were used for drug efficacy trial, by using completely randomized design. Results The overall prevalence of sheep lice in this study was 48%. Hair length (OR = 2, P = 0.00), body condition (OR = 1.9, P = 0.02), agro ecology (OR = 1.19, P = 0.00) and season (OR = 2, P = 0.01) were significantly (P < 0.05) associated with sheep lice infestation. The dominant sheep lice species in the study area were Bovicola ovis with prevalence of 83% and mixed infestation of 17% Bovicola ovis with Linognatus ovillus. In this study, the efficacy of 60% Diazinon and 1% Ivermectin against Bovicola ovis were 97% and 81%, respectively. Conclusion The overall prevalence of sheep lice infestation in the present study area was higher, in which Bovicola ovis was predominant and resistant against 1% Ivermectin. Therefore, tailor made intervention is required with a view to reducing the prevalence of sheep lice infestation and addressing drug resistance in the study area.

Abstract 3261: Visualizing treatment response dynamics of an in vitro three-dimensional ovarian cancer model

2010 ◽  
Author(s):  
Conor L. Evans ◽  
Imran Rizvi ◽  
Jonathan Celli ◽  
Adnan Abu-Yousif ◽  
Johannes F. de Boer ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Treatment Response ◽  
Three Dimensional ◽  
Cancer Model ◽  
Response Dynamics

scholarly journals In vitro ovarian cancer model based on three-dimensional agarose hydrogel

2014 ◽  
Vol 5 ◽  
pp. 204173141352043 ◽  
Author(s):  
Guojie Xu ◽  
Fuqiang Yin ◽  
Huayu Wu ◽  
Xuefeng Hu ◽  
Li Zheng ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Three Dimensional ◽  
Cancer Model ◽  
Model Based ◽  
Agarose Hydrogel

scholarly journals Natural Hypoxia is Not a Limiting Factor in Evaluating the Novel Arylidene Derivative MLT-401 Against an In Vitro Colorectal Cancer Model

2018 ◽  
Vol 46 (5) ◽  
pp. 2082-2089 ◽  
Author(s):  
Ismaeel  Bin-Jaliah
Keyword(s):  
Colorectal Cancer ◽  
Cell Cycle ◽  
Cell Proliferation ◽  
Apoptotic Cell ◽  
Limiting Factor ◽  
Cancer Model ◽  
Hypoxic Conditions ◽  
Hct 116 ◽  
Hct 116 Cells

Background/Aims: Cancer cells in vivo develop resistance to many anti-tumor drugs. One known factor to influence such drug resistance is hypoxia, which is an important component of the tumor microenvironment. Standard cancer lines mostly do not exhibit a cellular hypoxic microenvironment and there is a paucity of information on the efficacy of lead molecules in both cellular- and environment-induced hypoxic conditions. Therefore, in the present study, we have evaluated the efficacy of the arylidene derivative MLT-401, a lead molecule showing activity against colorectal cancer model using the HCT 116 cell line and CCD-80-C control cells in normoxic and natural (marginal) hypoxic conditions, which is usually observed in high-altitude regions. Methods: The efficacy of MLT-401 on HCT 116 and CCD-80-C cells were tested in both normoxia and marginal hypoxia conditions. MTT assay was used to evaluate cell proliferation, Annexin V binding assay for apoptotic cell quantification and PI staining for cell cycle were done by flow cytometry. Induction of pro-apoptotic marker BAX and anti-apoptotic Bcl-2 were assessed by western blot. Bcl-2/BAX ratio was calculated based on protein expression by western blotting and bands were quantified by Image J software. Results: Analysis of cell proliferation showed an average 10-fold reduction in the inhibition of HCT 116 cells in hypoxic conditions with approximately 500 nM MLT-401, while there was no significant change noted in marginal hypoxic conditions. A proportionate increase in the number of apoptotic cells and large M4 fraction of 10.5% and 26.7% of HCT116 against 6.3% of control cells in cell cycle assessment with MLT-401 concentrations ranging from 250 to 500 nM respectively clearly demonstrated anti-cancer activity. A Bcl-2/BAX ratio of < 1 showed that the induction of apoptosis was the gross mechanism underlying the inhibition of HCT 116 cells by MLT-401. Conclusion: Collectively, these results show MLT-401 as an effective anti-colorectal cancer lead molecule irrespective of normoxia or natural hypoxia.

A Human Corneal Equivalent Constructed from SV40-immortalised Corneal Cell Lines

2005 ◽  
Vol 33 (1) ◽  
pp. 37-45 ◽  
Author(s):  
Michaela Zorn-Kruppa ◽  
Svitlana Tykhonova ◽  
Gazanfer Belge ◽  
Jürgen Bednarz ◽  
Horst A. Diehl ◽  
...  
Keyword(s):  
Cell Lines ◽  
Cross Sections ◽  
Three Dimensional ◽  
Morphological Characteristics ◽  
Drug Efficacy ◽  
Collagen Matrix ◽  
Cell Types ◽  
In Vitro Models ◽  
Equivalent Model

Within the last decade, extensive research in the field of tissue and organ engineering has focused on the development of in vitro models of the cornea. The use of organotypic, three-dimensional corneal equivalents has several advantages over simple monolayer cultures. The aim of this study was to develop a corneal equivalent model composed of the same cell types as in the natural human tissue, but by using immortalised cell lines to ensure reproducibility and to minimise product variation. We report our success in the establishment of an SV40-immortalised human corneal keratocyte cell line (designated HCK). A collagen matrix, built up with these cells, displayed the morphological characteristics of the human stromal tissue and served as a biomatrix for the immortalised human corneal epithelial and endothelial cells. Histological cross-sections of the whole-cornea equivalents resemble human corneas in tissue structure. This organotypic in vitro model may serve as a research tool for the ophthalmic science community, as well as a model system for testing for eye irritancy and drug efficacy.

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