Chronic high glucose and insulin stimulate bone-marrow stromal cells adipogenic differentiation in young spontaneously hypertensive rats

2018 ◽  
Vol 233 (9) ◽  
pp. 6853-6865 ◽  
Author(s):  
Antonio H. Chaves Neto ◽  
Victor G. B. Brito ◽  
Thamine Landim de Barros ◽  
Caril C. F. do Amaral ◽  
Dóris H. Sumida ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
High Glucose ◽  
Bone Marrow Stromal Cells ◽  
Spontaneously Hypertensive Rats ◽  
Adipogenic Differentiation ◽  
Marrow Stromal Cells ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Stimulate Bone Marrow

Asiatic Acid Inhibits Adipogenic Differentiation of Bone Marrow Stromal Cells

2013 ◽  
Vol 68 (2) ◽  
pp. 437-442 ◽  
Author(s):  
Zheng-Wei Li ◽  
Cheng-dong Piao ◽  
Hong-Hui Sun ◽  
Xian-Sheng Ren ◽  
Yun-Shen Bai
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Adipogenic Differentiation ◽  
Marrow Stromal Cells ◽  
Asiatic Acid

scholarly journals Effects of Transplanting Bone Marrow Stromal Cells Transfected with CXCL13 on Fracture Healing of Diabetic Rats

2018 ◽  
Vol 49 (1) ◽  
pp. 123-133 ◽  
Author(s):  
Hui Jiang ◽  
Yicun Wang ◽  
Jia Meng ◽  
Shuo Chen ◽  
Jun Wang ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Fracture Healing ◽  
Stromal Cells ◽  
High Glucose ◽  
Bone Marrow Stromal Cells ◽  
Diabetic Rats ◽  
Marrow Stromal Cells ◽  
Area Measurement ◽  
Mineral Density

Background/Aims: Diabetic fracture have poor treatment and serious complications. Therefore, how to treat diabetic fracture is receiving increasing attention. This study aimed to investigate the effects of transplanting CXCL13-stimulated bone marrow stromal cells (BMSCs) on the fracture healing in diabetic rats. Methods: In vitro, RT-PCR was employed to examine the expression of CXCL13 in BMSCs in high glucose environment. MTT assay and apoptosis assay were utilized to determine the effects of CXCL13 overexpression on the proliferation and apoptosis of BMSCs respectively. ALP staining was applied to detect the ALP activity. In vivo, CXCL13-stimulated BMSCs were transplanted into the fracture sites of diabetic rats. At the 1st week, 2nd weeks, 4th week and 6th week after the operation, bone mineral density (BMD) and callus area measurement, ELISA detection, and HE staining were performed to evaluate the fracture healing. Results: Low BMD and less area of callus in diabetic rats showed that the recovery after fracture was worse in diabetic rats than in non-diabetic rats. Meanwhile, the expression of CXCL13 in serum was lower in diabetic rats than in non-diabetic rats. Overexpression of CXCL13 promoted the proliferation of BMSCs in vitro high glucose environment. After BMSCs transfected with CXCL13 being transplanted into the fracture sites of diabetic rats, it was found that the fracture healing was enhanced and ALP expression in serum became higher. HE staining results further verified the effects of transplantation of BMSCs transfected with CXCL13 on fracture healing in diabetic rats. Conclusion: These finding indicated that CXCL13 may play a critical role in the process of fracture healing, which could provide a deeper insight into molecular targets for the fracture healing in diabetic people.

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