Effects of shRNA‐mediated silencing of PSMA7 on cell proliferation and vascular endothelial growth factor expression via the ubiquitin‐proteasome pathway in cervical cancer

2018 ◽  
Author(s):  
Chen‐Chen Ren ◽  
Li Yang ◽  
Ling Liu ◽  
Yan‐Nan Chen ◽  
Guo‐Mei Cheng ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Vascular Endothelial Growth Factor ◽  
Cell Proliferation ◽  
Vascular Endothelial ◽  
Ubiquitin Proteasome Pathway ◽  
Factor Expression ◽  
Ubiquitin Proteasome ◽  
Proteasome Pathway ◽  
Growth Factor Expression ◽  
Endothelial Growth Factor

scholarly journals Effects of Tetrahydrocurcumin on Hypoxia-Inducible Factor-1αand Vascular Endothelial Growth Factor Expression in Cervical Cancer Cell-Induced Angiogenesis in Nude Mice

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Bhornprom Yoysungnoen ◽  
Parvapan Bhattarakosol ◽  
Suthiluk Patumraj ◽  
Chatchawan Changtam
Keyword(s):  
Cervical Cancer ◽  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Nude Mice ◽  
Hypoxia Inducible Factor ◽  
Vehicle Group ◽  
Vascular Endothelial ◽  
Factor Expression ◽  
Growth Factor Expression ◽  
Endothelial Growth Factor

Tetrahydrocurcumin (THC), one of the importantin vivometabolites of curcumin, inhibits tumor angiogenesis. Its effects on angiogenesis in cervical cancer- (CaSki-) implanted nude mice and its mechanisms on hypoxia-inducible factor-1αand vascular endothelial growth factor expression were investigated. Female BALB/c nude mice were divided into control (CON) and CaSki-implanted groups (CaSki group). One month after the injection with cervical cancer cells, mice were orally administered vehicle or 100, 300, and 500 mg/kg of THC daily for 30 consecutive days. The microvascular density (MVD) was evaluated using the CD31 expression. VEGF, VEGFR-2, and HIF-1αexpression were also detected by immunohistochemistry. The MVD in CaSki + vehicle group was significantly increased compared to the CON + vehicle group. Interestingly, when treated with THC at all doses, the CaSki group showed a significant smaller number of the MVD. The CaSki + vehicle group also showed significantly increased VEGF, VEGFR-2, and HIF-1αexpressions, but they were downregulated when mice were treated with THC at all doses. THC demonstrated an inhibitory effect against tumor angiogenesis in CaSki-implanted nude mice model. This effect is likely to be mediated by the downregulation of HIF-1-α, VEGF expression, and its receptor. THC could be developed into a promising agent for cancer therapy in the future.

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