Ptn functions downstream of C/EBPβ to mediate the effects of cAMP on uterine stromal cell differentiation through targeting Hand2 in response to progesterone

2017 ◽  
Vol 233 (2) ◽  
pp. 1612-1626 ◽  
Author(s):  
Hai-Fan Yu ◽  
Ran Tao ◽  
Zhan-Qing Yang ◽  
Kai Wang ◽  
Zhan-Peng Yue ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Stromal Cell ◽  
Uterine Stromal Cell

scholarly journals HB-EGF Ameliorates Oxidative Stress-Mediated Uterine Decidualization Damage

2019 ◽  
Vol 2019 ◽  
pp. 1-15 ◽  
Author(s):  
Hai-Fan Yu ◽  
Cui-Cui Duan ◽  
Zhan-Qing Yang ◽  
Yu-Si Wang ◽  
Zhan-Peng Yue ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Differentiation ◽  
Stromal Cell ◽  
Chain Complex ◽  
Mitochondrial Respiratory Chain ◽  
Mtdna Copy Number ◽  
Mitochondrial Respiratory Chain Complex ◽  
Mitochondrial Superoxide ◽  
Antioxidant Function ◽  
Uterine Stromal Cell

HB-EGF is essential for uterine decidualization, but its antioxidant function remains largely unclear. Here, we found that HB-EGF promoted the proliferation of stromal cells followed by the accelerated transition of the cell cycle from G1 to S phase and enhanced the expression or activity of Prl8a2, Prl3c1, and ALP which were well-established markers for uterine stromal cell differentiation during decidualization. Under oxidative stress, stromal cell differentiation was impaired, but this impairment was abrogated by rHB-EGF accompanied with the reduced levels of ROS and MDA which were regarded as the biomarkers for oxidative stress, indicating an antioxidant role of HB-EGF. Further analysis revealed that HB-EGF enhanced the activities of antioxidant enzymes SOD, CAT, and GPX, where addition of GPX inhibitor MS attenuated the induction of rHB-EGF on Prl8a2, Prl3c1, and ALP. Meanwhile, HB-EGF rescued the content of GSH and restored the ratio of GSH/GSSG after exposure to H2O2 but did not alter NOX activity. Along with a decline for mitochondrial superoxide, exogenous rHB-EGF improved the damage of oxidative stress on mtDNA copy number, ATP level, mitochondrial membrane potential, and activities of mitochondrial respiratory chain complex I and III whose blockage by ROT and AA led to a failure of rHB-EGF in protecting stromal cell differentiation against injury. Moreover, HB-EGF prevented stromal cell apoptosis by inhibiting Caspase-3 activity and Bax expression and recovering the level of Bcl-2 mRNA. Collectively, HB-EGF might ameliorate oxidative stress-mediated uterine decidualization damage.

scholarly journals Stimulation of a rat uterine stromal cell line in culture reveals a molecular switch for endocrine-dependent differentiation

2005 ◽  
Vol 184 (1) ◽  
pp. 119-127 ◽  
Author(s):  
V Rider ◽  
T Potapova ◽  
G Dai ◽  
M J Soares
Keyword(s):  
Cell Differentiation ◽  
Stromal Cells ◽  
Stromal Cell ◽  
Cell Model ◽  
Molecular Switch ◽  
Luciferase Expression ◽  
Decidual Cell ◽  
Uterine Stromal Cell ◽  
Interleukin 11 ◽  
Low Serum

Differentiation of uterine stromal cells is critical for the establishment of pregnancy. This study had two purposes: (i) to validate the use of the UIII rat uterine stromal cell model for investigating mechanisms underlying decidual cell differentiation, and (ii) to use this cell model to identify a molecular switch for cellular entry into the decidual cell differentiation pathway. Quiescent rat uterine stromal cells were transfected with a 500 bp segment of the decidual prolactin-related protein (dPRP) promoter ligated to a luciferase reporter gene. Cells were incubated in low-serum medium, or in low-serum medium containing progesterone (1 μM), estradiol 17-β (10 nM), cholera toxin (10 ng/ml) and interleukin-11 (10 ng/ml). Protein extracts were collected 48 h later and luciferase was measured in the cellular lysates. Cholera toxin and interleukin-11 stimulated luciferase expression (P< 0.05) and addition of sex steroids further increased (P< 0.05) dPRP promoter activity. Stromal cells did not proliferate (P< 0.05) under differentiation conditions. Deletion analysis of the dPRP promoter revealed maximal luciferase expression between −250 and −500 bp relative to the transcription start site. Comparison of cyclin E/Cdk2 activity between proliferating and differentiating cells showed a 3-fold increase (P< 0.05) at 12 h in differentiating cells. The results suggest that cyclin E/Cdk2 serves as a molecular switch for uterine stromal cell entry into the decidual cell differentiation pathway.

The support of bone marrow stromal cell differentiation by airbrushed nanofiber scaffolds

Biomaterials ◽  
2013 ◽  
Vol 34 (10) ◽  
pp. 2389-2398 ◽  
Author(s):  
Wojtek Tutak ◽  
Sumona Sarkar ◽  
Sheng Lin-Gibson ◽  
Tanya M. Farooque ◽  
Giri Jyotsnendu ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Cell Differentiation ◽  
Stromal Cell ◽  
Bone Marrow Stromal Cell ◽  
Marrow Stromal Cell ◽  
Nanofiber Scaffolds

Microbioreactors for Raman Microscopy of Stromal Cell Differentiation

2010 ◽  
Vol 82 (5) ◽  
pp. 1844-1850 ◽  
Author(s):  
Vishnu Vardhan Pully ◽  
Aufried Lenferink ◽  
Henk-Jan van Manen ◽  
Vinod Subramaniam ◽  
Clemens A. van Blitterswijk ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Stromal Cell ◽  
Raman Microscopy

scholarly journals Tensile Loading Modulates Bone Marrow Stromal Cell Differentiation and the Development of Engineered Fibrocartilage Constructs

2010 ◽  
Vol 16 (6) ◽  
pp. 1913-1923 ◽  
Author(s):  
John T. Connelly ◽  
Eric J. Vanderploeg ◽  
Janna K. Mouw ◽  
Christopher G. Wilson ◽  
Marc E. Levenston
Keyword(s):  
Bone Marrow ◽  
Cell Differentiation ◽  
Stromal Cell ◽  
Bone Marrow Stromal Cell ◽  
Tensile Loading ◽  
Marrow Stromal Cell

scholarly journals Asporin Restricts Mesenchymal Stromal Cell Differentiation, Alters the Tumor Microenvironment, and Drives Metastatic Progression

2019 ◽  
Vol 79 (14) ◽  
pp. 3636-3650 ◽  
Author(s):  
Robert M. Hughes ◽  
Brian W. Simons ◽  
Hamda Khan ◽  
Rebecca Miller ◽  
Valentina Kugler ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Tumor Microenvironment ◽  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Metastatic Progression
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