Characterization of Runx2 phosphorylation sites required for TGF-β1-mediated stimulation of matrix metalloproteinase-13 expression in osteoblastic cells

2017 ◽  
Vol 233 (2) ◽  
pp. 1082-1094 ◽  
Author(s):  
Balasubramanian Arumugam ◽  
Mariappanadar Vairamani ◽  
Nicola C. Partridge ◽  
Nagarajan Selvamurugan
Keyword(s):  
Matrix Metalloproteinase ◽  
Osteoblastic Cells ◽  
Phosphorylation Sites ◽  
Matrix Metalloproteinase 13 ◽  
Tgf Β1 ◽  
Stimulation Of

Regulation of transforming growth factor-β1-stimulation of Runx2 acetylation for matrix metalloproteinase 13 expression in osteoblastic cells

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Kanagaraj Gomathi ◽  
Muthukumar Rohini ◽  
Nicola C. Partridge ◽  
Nagarajan Selvamurugan
Keyword(s):  
Growth Factor ◽  
Matrix Metalloproteinase ◽  
Transforming Growth Factor Beta ◽  
Target Genes ◽  
Transforming Growth Factor ◽  
Bone Development ◽  
Osteoblastic Cells ◽  
Post Translational Modification ◽  
Matrix Metalloproteinase 13 ◽  
Tgf Β1

Abstract Transforming growth factor beta 1 (TGF-β1) functions as a coupling factor between bone development and resorption. Matrix metalloproteinase 13 (MMP13) is important in bone remodeling, and skeletal dysplasia is caused by a deficiency in MMP13 expre-ssion. Runx2, a transcription factor is essential for bone development, and MMP13 is one of its target genes. TGF-β1 promoted Runx2 phosphorylation, which was necessary for MMP13 production in osteoblastic cells, as we previously shown. Since the phosphorylation of some proteins causes them to be degraded by the ubiquitin/proteasome pathway, we hypothesized that TGF-β1 might stabilize the phosphorylated Runx2 protein for its activity by other post-translational modification (PTM). This study demonstrated that TGF-β1-stimulated Runx2 acetylation in rat osteoblastic cells. p300, a histone acetyltransferase interacted with Runx2, and it promoted Runx2 acetylation upon TGF-β1-treatment in these cells. Knockdown of p300 decreased the TGF-β1-stimulated Runx2 acetylation and MMP13 expression in rat osteoblastic cells. TGF-β1-treatment stimulated the acetylated Runx2 bound at the MMP13 promoter, and knockdown of p300 reduced this effect in these cells. Overall, our studies identified the transcriptional regulation of MMP13 by TGF-β1 via Runx2 acetylation in rat osteoblastic cells, and these findings contribute to the knowledge of events presiding bone metabolism.

scholarly journals Characterization of an exosite binding inhibitor of matrix metalloproteinase 13

2007 ◽  
Vol 17 (1) ◽  
pp. 66-71 ◽  
Author(s):  
L. T. Gooljarsingh ◽  
A. Lakdawala ◽  
F. Coppo ◽  
L. Luo ◽  
G. B. Fields ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Matrix Metalloproteinase 13

scholarly journals Characterization of Selective Exosite-Binding Inhibitors of Matrix Metalloproteinase 13 That Prevent Articular Cartilage Degradation in Vitro

2014 ◽  
Vol 57 (22) ◽  
pp. 9598-9611 ◽  
Author(s):  
Timothy P. Spicer ◽  
Jianwen Jiang ◽  
Alexander B. Taylor ◽  
Jun Yong Choi ◽  
P. John Hart ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Matrix Metalloproteinase ◽  
Cartilage Degradation ◽  
Matrix Metalloproteinase 13 ◽  
Binding Inhibitors

FK506 suppresses the stimulation of matrix metalloproteinase 13 synthesis by interleukin-1β in rheumatoid synovial fibroblasts

2005 ◽  
Vol 98 (2) ◽  
pp. 194-199 ◽  
Author(s):  
Kiyoshi Migita ◽  
Taichiro Miyashita ◽  
Yumi Maeda ◽  
Takahiko Aoyagi ◽  
Yojiro Kawabe ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Synovial Fibroblasts ◽  
Interleukin 1Β ◽  
Matrix Metalloproteinase 13 ◽  
Stimulation Of
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