A prognostic 4-gene expression signature for squamous cell lung carcinoma

2017 ◽  
Vol 232 (12) ◽  
pp. 3702-3713 ◽  
Author(s):  
Jun Li ◽  
Jing Wang ◽  
Yanbin Chen ◽  
Lijie Yang ◽  
Sheng Chen
Keyword(s):  
Gene Expression ◽  
Squamous Cell ◽  
Lung Carcinoma ◽  
Gene Expression Signature ◽  
Squamous Cell Lung Carcinoma ◽  
Cell Lung Carcinoma ◽  
Expression Signature

scholarly journals Novel Candidate Key Drivers in the Integrative Network of Genes, MicroRNAs, Methylations, and Copy Number Variations in Squamous Cell Lung Carcinoma

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Tao Huang ◽  
Jing Yang ◽  
Yu-dong Cai
Keyword(s):  
Gene Expression ◽  
Lung Cancer ◽  
Dna Methylation ◽  
Squamous Cell ◽  
Lung Carcinoma ◽  
Copy Number ◽  
Squamous Cell Lung Carcinoma ◽  
Cell Lung Carcinoma ◽  
Key Drivers ◽  
Integrative Network

The mechanisms of lung cancer are highly complex. Not only mRNA gene expression but also microRNAs, DNA methylation, and copy number variation (CNV) play roles in tumorigenesis. It is difficult to incorporate so much information into a single model that can comprehensively reflect all these lung cancer mechanisms. In this study, we analyzed the 129 TCGA (The Cancer Genome Atlas) squamous cell lung carcinoma samples with gene expression, microRNA expression, DNA methylation, and CNV data. First, we used variance inflation factor (VIF) regression to build the whole genome integrative network. Then, we isolated the lung cancer subnetwork by identifying the known lung cancer genes and their direct regulators. This subnetwork was refined by the Bayesian method, and the directed regulations among mRNA genes, microRNAs, methylations, and CNVs were obtained. The novel candidate key drivers in this refined subnetwork, such as the methylation of ARHGDIB and HOXD3, microRNA let-7a and miR-31, and the CNV of AGAP2, were identified and analyzed. On three large public available lung cancer datasets, the key drivers ARHGDIB and HOXD3 demonstrated significant associations with the overall survival of lung cancer patients. Our results provide new insights into lung cancer mechanisms.

Anti-cancer gene expression profile of curcumin identifies new therapeutic targets in squamous cell lung carcinoma in vitro

2007 ◽  
Vol 25 (18_suppl) ◽  
pp. 18203-18203
Author(s):  
S. Sen ◽  
A. Singh ◽  
A. Pal ◽  
C. Sharma ◽  
R. Kar ◽  
...  
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Squamous Cell ◽  
Lung Carcinoma ◽  
Mtt Assay ◽  
Squamous Cell Lung Carcinoma ◽  
Rt Pcr ◽  
Curcumin Treatment ◽  
Cell Lung Carcinoma

18203 Background: Lung cancer is notorious for early metastasis and very high mortality, worldwide. Curcumin, a chemopreventive has also shown chemotherapeutic potential but its mechanisms are still not well understood. In this study, the genes targeted by Curcumin were investigated to identify new targets for the therapy of squamous cell lung carcinoma (SCC) in vitro. Methods: Lung squamous cell carcinoma cells (H520) were cultured in DMEM with 10% FCS. They were treated with Curcumin (25μM) for 24 hours. Apoptosis was detected by morphological examination, MTT assay, flowcytometry and TUNEL assay. Microarray analysis of gene expression profiles on curcumin treatment was done. Real time quantitative RT-PCR confirmed the results. Results: Curcumin (25μM for 24 hours) caused 29.8 ± 2.1% cytotoxicity (MTT assay). Apoptosis was corroborated by flowcytometry (23.7 ± 1.4%) and TUNEL (21.6 ± 1.8%). Using microarray analysis, 34 genes were seen to be upregulated and 31 genes downregulated after curcumin treatment. Several apoptosis related genes were upregulated including GADD45a (3.36 fold), transcription factor Egr-1 (2.2 fold) and Peroxiredoxin-I (2 fold). In addition, Angiopoietin-2 (Ang-2), an angiogenic factor that promotes angiogenesis and tumor invasion was downregulated (1.7 fold). Real time quantitative RT-PCR confirmed the results. Conclusions: This study helps to identify novel putative intervention sites as targets for curcumin in the therapy of squamous cell lung carcinoma (SCC) in vitro and can contribute to better understanding of lung tumorigenesis and anticancer therapy. No significant financial relationships to disclose.

O53. Multicenter validation of a lymph node metastasis gene expression signature for head and neck squamous cell carcinomas

Oral Oncology ◽  
2011 ◽  
Vol 47 ◽  
pp. S46
Author(s):  
F.K.J. Leusink ◽  
P.J. Slootweg ◽  
M.W.M. van den Brekel ◽  
R.J. Baatenburg de Jong ◽  
F.C.P. Holstege ◽  
...  
Keyword(s):  
Gene Expression ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Head And Neck ◽  
Squamous Cell ◽  
Gene Expression Signature ◽  
Squamous Cell Carcinomas ◽  
Expression Signature ◽  
Node Metastasis ◽  
Multicenter Validation
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