Osteogenic differentiation of human gingival mesenchymal stem cells byAristolochia bracteolatasupplementation through enhanced Runx2 expression

2017 ◽  
Vol 232 (7) ◽  
pp. 1591-1595 ◽  
Author(s):  
Dinesh Murugan Girija ◽  
Suresh Y Ranga Rao ◽  
Mangathayaru Kalachaveedu ◽  
Rajasekaran Subbarayan
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Runx2 Expression

miRNA-126 Inhibits Osteogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells (BMSCs)

2022 ◽  
Vol 12 (4) ◽  
pp. 794-799
Author(s):  
Le Chang ◽  
Wei Duan ◽  
Chuang Wang ◽  
Jian Zhang
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Mrna Level ◽  
Alizarin Red ◽  
Runx2 Expression ◽  
Alp Activity ◽  
Smad4 Protein ◽  
Marrow Mesenchymal Stem Cells

This study was to determine whether microRNA (miRNA)-126 regulates osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). Rat BMSCs were extracted and stimulated for osteogenic differentiation. Functional experiments were conducted to assess miR-126’s impact on BMSCs differentiation. Western blot and RT-qPCR determined miR-126 expression. ALP activity detection and alizarin red staining detection were also performed. After osteogenic differentiation of BMSCs, miR-126 expression was gradually decreased over time. Overexpression of miR-26 decreased ALP activity, Notch signaling activity as well as declined Runx2 expression and calcium Salt nodules after treatment. Importantly, we found that Smad4 serves as a target of miR-126 while upregulation of the miRNA was accompanied with the decreased Smad4 protein expression without affecting the Smad4 mRNA level. In conclusion, miR-126 restrains osteogenic differentiation through inhibition of SMAD4 signaling, providing a novel insight into the mechanism.

Exosome-Derived miR21/SMAD7 Derived from Donor Osteoporosis Mesenchymal Stem Cells Inhibits Osteogenic Differentiation of Reconstituted Hosts

2019 ◽  
Vol 9 (10) ◽  
pp. 1346-1354
Author(s):  
Jie Chen ◽  
Yongsheng Luo ◽  
Ting Li ◽  
Wenbo Yang ◽  
Wen Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Runx2 Expression ◽  
Alp Activity ◽  
Marrow Mesenchymal Stem Cells ◽  
Smad7 Expression ◽  
Different Sources ◽  
Adult Mscs ◽  
Normal Adults

Exogenous bone marrow mesenchymal stem cells (MSCs) can regulate osteogenic differentiation. MicroRNA-21 has been shown to target SMAD7. This study explored the mechanism by which miR-21/SMAD7 inhibits osteogenic differentiation from exosomes secreted by osteoporosis patients-derived MSCs. Exosomes were obtained from MSCs and miR-21 expression was detected. Normal MSCs were treated with exosomes secreted by MSCs from different sources for osteogenic differentiation followed by detection of ALP, Bglap and Runx2 level and ALP activity. Normal MSCs were divided into three groups, which were treated with exosomes from normal adult MSCs, osteoporosis patients-derived MSCs and osteoporosis patients-derived MSCs + SMAD7 overexpression followed by analysis of the mRNA expression of ALP, Bglap and Runx2 by qRT-PCR and ALP activity. miR-21 expression in exosomes from osteoporosis patients-derived MSCs was significantly higher than that from normal adults MSCs. After treatment with exosomes from osteoporosis patients-derived MSCs, Runx2 expression and ALP activity was significantly decreased. SMAD7 expression in osteoporosis patients was significantly lower than that in normal adults. The expression of ALP, Bglap and Runx2 is significantly decreased after overexpression of SMAD7. SMAD7 is a target gene of miR-21 and plays a role in inhibiting osteogenic differentiation induced by exosomes from osteoporosis-derived MSCs.

scholarly journals Activation of transcriptional factor ZBTB16 expression during osteogenic differentiation of mesenchymal stem cells

2021 ◽  
Vol 10 (3) ◽  
pp. 44-55
Author(s):  
D. S. Semenova ◽  
A. M. Kiselev ◽  
A. B. Malashicheva
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Aortic Valve ◽  
Osteogenic Differentiation ◽  
Dental Pulp ◽  
Interstitial Cells ◽  
Transcriptional Factor ◽  
Runx2 Expression ◽  
Valve Interstitial Cells ◽  
Different Types

Aim. Calcified aortic valve stenosis is the third leading cause of cardiovascular disease. The mechanisms underlying this process remain unclear, however, it is known that they are largely similar to the formation of bone tissue during embryonic development, as well as in the postnatal period during regeneration. There is evidence for the             involvement of Zinc Finger and BTB Domain Containing 16 (ZBTB16) in skeletal development. At the same time, a number of studies carried out on different types of cell cultures indicate a contradictory and ambiguous effect of ZBTB16 on RUNX2 expression. Thus, the aim of this study was to investigate the dynamic variability of ZBTB16 expression, as well as its role in aortic valve calcification.Methods. The study used different types of mesenchymal cells cultures - aortic valve interstitial cells, umbilical cord mesenchymal stem cells, ligament stem cells and dental pulp stem cells. Changes in ZBTB16 and RUNX2 expression levels                under the influence of osteogenic stimuli, as well as during exogenous activation of ZBTB16, were analyzed using real-time PCR. Expression levels of some osteogenic markers - BMP2,4, COL1A1, IBSP, DLX2, PDK4 - were analyzed in the interstitial cells of the aortic valve.Results. The results of the study indicate that a significant increase in the expression of ZBTB16 is observed during the induction of osteogenic differentiation of various cell cultures - interstitial cells of the aortic valve, mesenchymal stem cells of           the umbilical cord, stem cells of the ligaments and dental pulp. Apparently, the processes of osteogenic differentiation of aortic valve interstitial cells, in the presence of dexamethasone in cultivation medium, are provided through RUNX2-dependent signaling for the further activation of osteogenic markers.Conclusion. The study of modulation of cellular signals by ZBTB16, when activating or suppressing the work of a transcriptional factor, in the future may bring us closer to the ability to enhance the regenerative abilities of bone tissue cells or, conversely, prevent calcification of the aortic valve tissues.

Exosome Secreted from Mesenchymal Stem Cells (MSCs) of Osteoporosis Inhibits the Osteogenic Differentiation

2022 ◽  
Vol 12 (5) ◽  
pp. 1022-1027
Author(s):  
Liangbang Wu ◽  
Xinqiang Wang ◽  
Yuehong Zhang ◽  
Zhenhai Hou ◽  
Longbao Zheng ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Target Gene ◽  
Runx2 Expression ◽  
Alp Activity

This study analyze the effect of exosome secreted from MSCs on osteogenic differentiation in OP rats. The exosome was obtained from cultivated MSCs isolated from OP rats with ultracentrifugation. OP rats were treated with exosome secreted from MSCs of normal rats, exosome secreted from MSCs of OP rats and exosome secreted from MSCs of OP rats with overexpression of ALP followed by analysis of the osteogenic differentiation, the expression of ALP, Bglap and Runx2 and the targeted correlation between miR-351 and ALP. The MSCs in normal rats and OP rats were able to adhere to wall. There was elongated. The level of miR-351 in OP rats was significantly higher than normal rats. The Runx2 expression and ALP activity in rats treated with exosome secreted from MSCs of OP rats was declined significantly compared to that from MSCs of normal rats. ALP was a target gene of miR-351. In conclusion, the exosome secreted from MSCs of OP rats inhibits the osteogenic differentiation possibly through restraining miR-351-ALP.

BMP9 Regulates Osteogenic Differentiation of Mesenchymal Stem Cells Through JNKs Kinase Pathway

2013 ◽  
Vol 40 (12) ◽  
pp. 1220
Author(s):  
Jing XU ◽  
Dan ZHAO ◽  
Jian WANG ◽  
WenJuan WANG ◽  
JinYong LUO
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Kinase Pathway

Wnt7a Promotes Osteogenic Differentiation of Human Mesenchymal Stem Cells

2019 ◽  
Author(s):  
Leiluo Yang ◽  
Qing Li ◽  
Junhong Zhang ◽  
Pengcheng Li ◽  
Chaoliang Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Human Mesenchymal Stem Cells
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