Acetylcholine Attenuates Hypoxia/Reoxygenation Injury by Inducing Mitophagy Through PINK1/Parkin Signal Pathway in H9c2 Cells

2015 ◽  
Vol 231 (5) ◽  
pp. 1171-1181 ◽  
Author(s):  
Lei Sun ◽  
Mei Zhao ◽  
Yang Yang ◽  
Run-Qing Xue ◽  
Xiao-Jiang Yu ◽  
...  
Keyword(s):  
Signal Pathway ◽  
H9c2 Cells ◽  
Reoxygenation Injury ◽  
Hypoxia Reoxygenation

scholarly journals Naringin Effectively Protects Cardiomyocytes Against Hypoxia/Reoxygenation Injury

2021 ◽  
Vol 37 (3) ◽  
Author(s):  
Vu Thi Thu ◽  
Ngo Thi Hai Yen
Keyword(s):  
Membrane Potential ◽  
Mitochondrial Membrane Potential ◽  
Mitochondrial Membrane ◽  
Cell Group ◽  
H9c2 Cell ◽  
H9c2 Cells ◽  
H9c2 Cardiomyocytes ◽  
Reoxygenation Injury ◽  
Hypoxia Reoxygenation

This study was conducted to evaluate the protective effect of Naringin (NAR) on H9C2 cardiomyocytes in hypoxia/reoxygenation (HR) injury in vitro induced by the hypoxia chamber. Methods: H9C2 cells were grown under normal (control) and HR conditions. The viability, cardiolipin content and mitochondrial membrane potential of H9C2 cells in experimental groups were analyzed by using suitable kits. Results: The obtained results showed that the addition of Naringin (16÷160 µM) significantly increased the survival rate of H9C2 cells under HR conditions. In particular, NAR had the highest efficiency in preserving mitochondrial function at concentrations of 80 µM and 160 µM. In HR-exposed H9C2 cell group, the cardiolipin content and mitochondrial membrane potential values of H9C2 cells were decreased sharply with that of control (71,64±1,37% and 68,12±2,78%, p<0,05). Interestingly, mitochondrial cardiolipin contents were signigicantly increased in H9C2 cells post-hypoxic treated wtih NAR at dose of 80 µM 160 µM to 87,76±1,89% and 81,09±1,21%. Additionally, post-hypoxic supplementation of NAR at concentration of 80 µM and 160 µM effectively increased mitochondrial membrane potential values. Conclusion: The obtained results are preliminary data on the effects of NAR in protecting mitochondrial-targeted cardiomyocytes against HR injury.

scholarly journals Knockdown of forkhead box protein P1 alleviates hypoxia reoxygenation injury in H9c2 cells through regulating Pik3ip1/Akt/eNOS and ROS/mPTP pathway

Bioengineered ◽  
2022 ◽  
Vol 13 (1) ◽  
pp. 1320-1334
Author(s):  
Xinming Liu ◽  
Yixing Yang ◽  
Jiawei Song ◽  
Dongjie Li ◽  
Xiaoyan Liu ◽  
...  
Keyword(s):  
H9c2 Cells ◽  
Forkhead Box ◽  
Reoxygenation Injury ◽  
Hypoxia Reoxygenation

scholarly journals Acetylcholine Mediates AMPK-Dependent Autophagic Cytoprotection in H9c2 Cells During Hypoxia/Reoxygenation Injury

2013 ◽  
Vol 32 (3) ◽  
pp. 601-613 ◽  
Author(s):  
Mei Zhao ◽  
Lei Sun ◽  
Xiao-Jiang Yu ◽  
Yi Miao ◽  
Jin-Jun Liu ◽  
...  
Keyword(s):  
H9c2 Cells ◽  
Reoxygenation Injury ◽  
Hypoxia Reoxygenation
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