The Purα/Purβ Single-Strand DNA-Binding Proteins Attenuate Smooth-Muscle Actin Gene Transactivation in Myofibroblasts

2014 ◽  
Vol 229 (9) ◽  
pp. 1256-1271 ◽  
Author(s):  
Seethalakshmi Hariharan ◽  
Robert J. Kelm ◽  
Arthur Roger Strauch
Keyword(s):  
Smooth Muscle ◽  
Dna Binding ◽  
Binding Proteins ◽  
Smooth Muscle Actin ◽  
Dna Binding Proteins ◽  
Single Strand ◽  
Actin Gene ◽  
Muscle Actin ◽  
Muscle Actin Gene

Chromosomal mapping of the human smooth muscle actin gene (enteric type, ACTA3) to 2p13.1 and molecular nature of the HindIII polymorphism

Genomics ◽  
1995 ◽  
Vol 25 (3) ◽  
pp. 720-723 ◽  
Author(s):  
Hisao Ueyama ◽  
Johji Inazawa ◽  
Hoyoku Nishino ◽  
Deng Han-Xiang ◽  
Yukiko Ochiai ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Actin ◽  
Chromosomal Mapping ◽  
Actin Gene ◽  
Muscle Actin ◽  
Muscle Actin Gene ◽  
Hindiii Polymorphism ◽  
Molecular Nature

scholarly journals A 29-nucleotide DNA segment containing an evolutionarily conserved motif is required in cis for cell-type-restricted repression of the chicken alpha-smooth muscle actin gene core promoter.

1988 ◽  
Vol 8 (1) ◽  
pp. 241-250 ◽  
Author(s):  
S L Carroll ◽  
D J Bergsma ◽  
R J Schwartz
Keyword(s):  
Smooth Muscle ◽  
Core Promoter ◽  
Smooth Muscle Actin ◽  
Gene Promoter ◽  
Actin Gene ◽  
Muscle Actin ◽  
Alpha Smooth Muscle Actin ◽  
Actin Genes ◽  
Muscle Actin Gene ◽  
Ccaat Box

A series of 5' deletion mutations of the upstream flanking sequences of the chicken alpha-smooth muscle (aortic) actin gene was prepared and inserted into the chloramphenicol acetyltransferase expression vector pSV0CAT. Deletion recombinants were transfected into fibroblasts, which actively express the alpha-smooth muscle actin gene, and primary myoblast cultures, which accumulate much lower quantities of alpha-smooth muscle actin mRNAs. The first 122 nucleotides of 5'-flanking DNA were found to contain a "core" promoter capable of accurately directing high levels of transcription in both fibroblasts and myotubes. The activity of this core promoter is modulated in fibroblasts by a "governor" element(s) located at least in part between nucleotides -257 and -123. This region contains sequences potentially conserved between mammalian and avian alpha-smooth muscle actin genes as well as one of a pair of 16-base-pair inverted CCAAT box-associated repeats which are conserved among all chordate muscle actin genes examined to date. A smaller DNA segment (-151 to -123) containing these upstream CCAAT box-associated repeats was sufficient to suppress expression of the core promoter in muscle cultures, suggesting that the upstream CCAAT box-associated repeats play a negative role in the alpha-smooth muscle actin gene promoter.

A 29-nucleotide DNA segment containing an evolutionarily conserved motif is required in cis for cell-type-restricted repression of the chicken alpha-smooth muscle actin gene core promoter

1988 ◽  
Vol 8 (1) ◽  
pp. 241-250
Author(s):  
S L Carroll ◽  
D J Bergsma ◽  
R J Schwartz
Keyword(s):  
Smooth Muscle ◽  
Core Promoter ◽  
Smooth Muscle Actin ◽  
Gene Promoter ◽  
Actin Gene ◽  
Muscle Actin ◽  
Alpha Smooth Muscle Actin ◽  
Actin Genes ◽  
Muscle Actin Gene ◽  
Ccaat Box

A series of 5' deletion mutations of the upstream flanking sequences of the chicken alpha-smooth muscle (aortic) actin gene was prepared and inserted into the chloramphenicol acetyltransferase expression vector pSV0CAT. Deletion recombinants were transfected into fibroblasts, which actively express the alpha-smooth muscle actin gene, and primary myoblast cultures, which accumulate much lower quantities of alpha-smooth muscle actin mRNAs. The first 122 nucleotides of 5'-flanking DNA were found to contain a "core" promoter capable of accurately directing high levels of transcription in both fibroblasts and myotubes. The activity of this core promoter is modulated in fibroblasts by a "governor" element(s) located at least in part between nucleotides -257 and -123. This region contains sequences potentially conserved between mammalian and avian alpha-smooth muscle actin genes as well as one of a pair of 16-base-pair inverted CCAAT box-associated repeats which are conserved among all chordate muscle actin genes examined to date. A smaller DNA segment (-151 to -123) containing these upstream CCAAT box-associated repeats was sufficient to suppress expression of the core promoter in muscle cultures, suggesting that the upstream CCAAT box-associated repeats play a negative role in the alpha-smooth muscle actin gene promoter.

Structure of a human smooth muscle actin gene (aortic type) with a unique intron site

1984 ◽  
Vol 4 (6) ◽  
pp. 1073-1078
Author(s):  
H Ueyama ◽  
H Hamada ◽  
N Battula ◽  
T Kakunaga
Keyword(s):  
Smooth Muscle ◽  
Amino Acid ◽  
Smooth Muscle Actin ◽  
Amino Acid Sequences ◽  
Actin Gene ◽  
Muscle Actin ◽  
Aortic Smooth Muscle ◽  
Actin Genes ◽  
Muscle Actin Gene ◽  
Normal Human

A recombinant phage containing an actin gene (lambda Ha201) was isolated from a human DNA library and the structure of the actin gene was determined. The amino acid sequences deduced from the nucleotide sequences of lambda Ha201 were compared with those of six actin isoforms; they matched those of bovine aortic smooth muscle actin, except for codon 309, which was valine (GTC) in lambda Ha201 and alanine (GCN) in bovine aortic smooth muscle actin. Southern blot hybridization experiments showed that the gene of normal human cells did not have the TaqI-sensitive site around position 309, whereas half of the genes of HUT14 cells did. These results indicate that one allele of the aortic smooth muscle actin gene in HUT14 cells has a transition point mutation (C----T) at codon 309 and that the amino acid sequences of normal human aorta and bovine smooth muscle actins are probably identical. In addition to the five introns interrupting exons at codons 150, 204, and 267, and between codons 41 and 42 and 327 and 328, which are common to skeletal muscle and cardiac muscle actin genes, the smooth muscle actin gene has two more intron sites between codons 84 and 85 and 121 and 122. The previously unreported intron site between codons 84 and 85 is unique to the smooth muscle actin gene. The intron site between codons 121 and 122 is common to beta-actin genes but is not found in other muscle actin genes. A hypothesis is proposed for the evolutionary pathway of the actin gene family.

scholarly journals Structure of a human smooth muscle actin gene (aortic type) with a unique intron site.

1984 ◽  
Vol 4 (6) ◽  
pp. 1073-1078 ◽  
Author(s):  
H Ueyama ◽  
H Hamada ◽  
N Battula ◽  
T Kakunaga
Keyword(s):  
Smooth Muscle ◽  
Amino Acid ◽  
Smooth Muscle Actin ◽  
Amino Acid Sequences ◽  
Actin Gene ◽  
Muscle Actin ◽  
Aortic Smooth Muscle ◽  
Actin Genes ◽  
Muscle Actin Gene ◽  
Normal Human

A recombinant phage containing an actin gene (lambda Ha201) was isolated from a human DNA library and the structure of the actin gene was determined. The amino acid sequences deduced from the nucleotide sequences of lambda Ha201 were compared with those of six actin isoforms; they matched those of bovine aortic smooth muscle actin, except for codon 309, which was valine (GTC) in lambda Ha201 and alanine (GCN) in bovine aortic smooth muscle actin. Southern blot hybridization experiments showed that the gene of normal human cells did not have the TaqI-sensitive site around position 309, whereas half of the genes of HUT14 cells did. These results indicate that one allele of the aortic smooth muscle actin gene in HUT14 cells has a transition point mutation (C----T) at codon 309 and that the amino acid sequences of normal human aorta and bovine smooth muscle actins are probably identical. In addition to the five introns interrupting exons at codons 150, 204, and 267, and between codons 41 and 42 and 327 and 328, which are common to skeletal muscle and cardiac muscle actin genes, the smooth muscle actin gene has two more intron sites between codons 84 and 85 and 121 and 122. The previously unreported intron site between codons 84 and 85 is unique to the smooth muscle actin gene. The intron site between codons 121 and 122 is common to beta-actin genes but is not found in other muscle actin genes. A hypothesis is proposed for the evolutionary pathway of the actin gene family.

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