Effect of Mechanical Strain on the Collagen VI Pericellular Matrix in Anterior Cruciate Ligament Fibroblasts

2014 ◽  
Vol 229 (7) ◽  
pp. 878-886 ◽  
Author(s):  
Francesca Sardone ◽  
Francesco Traina ◽  
Francesca Tagliavini ◽  
Camilla Pellegrini ◽  
Luciano Merlini ◽  
...  
Keyword(s):  
Anterior Cruciate Ligament ◽  
Cruciate Ligament ◽  
Mechanical Strain ◽  
Pericellular Matrix ◽  
Collagen Vi ◽  
Anterior Cruciate

Fibroblasts From Common Anterior Cruciate Ligament Tendon Grafts Exhibit Different Biologic Responses to Mechanical Strain

2020 ◽  
Vol 49 (1) ◽  
pp. 215-225
Author(s):  
Sebastian Cardona-Ramirez ◽  
Aaron M. Stoker ◽  
James L. Cook ◽  
Richard Ma
Keyword(s):  
Anterior Cruciate Ligament ◽  
Acl Reconstruction ◽  
Metabolic Activity ◽  
Cruciate Ligament ◽  
Mechanical Strain ◽  
Advantages And Disadvantages ◽  
Relative Paucity ◽  
Tendon Grafts ◽  
Anterior Cruciate

Background: Different tendons are chosen for anterior cruciate ligament (ACL) reconstruction based on perceived advantages and disadvantages, yet there is a relative paucity of information regarding biologic responsiveness of commonly used tendon grafts to mechanical strain. Purpose: To evaluate the in vitro responses of graft fibroblasts derived from tendons used for ACL reconstruction to clinically relevant strain levels. Study Design: Controlled laboratory study. Methods: Twelve quadriceps tendons (QTs), 12 patellar tendons (PTs), and 9 hamstring tendons (HTs) were harvested from skeletally mature dogs (n = 16). Tendon fibroblasts were isolated and seeded onto BioFlex plates (1 × 105 cells/well). Cells were subjected to 3 strain conditions (stress deprivation, 0%; physiologic, 4%; high, 10%) for 5 days. Media were collected for proinflammatory and metabolic assays. RNA was extracted for gene expression analysis using real-time reverse transcription polymerase chain reaction. Results: Stress deprivation elicited significantly higher metabolic activity from HT and PT cells than from QT cells ( P < .001 and P = .001, respectively). There were no differences in metabolic activity among all 3 graft fibroblasts at physiologic and high strain. COL-1 expression was significantly higher in PT versus HT during physiologic strain ( P = .007). No significant differences with COL-3 expression were seen. TIMP-1 ( P = .01) expression was higher in PT versus HT under physiologic strain. Scleraxis expression was higher in PT versus HT ( P = .007) under physiologic strain. A strain-dependent increase in PGE2 levels occurred for all grafts. At physiologic strain conditions, HT produced significantly higher levels of PGE2 versus QT ( P < .001) and PT ( P = .005). Conclusion: Fibroblasts from common ACL graft tissues exhibited different metabolic responses to mechanical strain. On the basis of these data, we conclude that early production of extracellular matrix and proinflammatory responses from ACL grafts are dependent on mechanical loading and graft source. Clinical Relevance: Graft-specific differences in ACL reconstruction outcomes are known to exist. Our results suggest that there are differences in the biologic responsiveness of cells from the tendon grafts used in ACL reconstruction, which are dependent on strain levels and graft source. The biologic properties of the tissue used for ACL reconstruction should be considered when selecting graft source.

scholarly journals Do We have an Aimer to put the Tibial and Femoral Tunnels in the Same Line to reduce Mechanical Strain in Reconstructed Anterior Cruciate Ligament?

2017 ◽  
Vol 03 (02) ◽  
pp. 130-131
Author(s):  
Khalifa Elmajri
Keyword(s):  
Anterior Cruciate Ligament ◽  
Cruciate Ligament ◽  
Mechanical Strain ◽  
Entry Point ◽  
Acl Graft ◽  
Tibial Insertion ◽  
Graft Tension ◽  
Postoperative Changes ◽  
Graft Tissue ◽  
Anterior Cruciate

AbstractThe strain between the ACL graft tissue and the osteochondral tissue at the tunnel entrances would lead to postoperative deviation of the femoral and/or tibial insertion of the ACL graft. Postoperative changes of the 3D orientation of the ACL graft affect its physiometry leading to failure of graft tension. A conception of an aimer is suggested to insure straightening of the intraosseous (tibial and femoral) and intra-articular parts of the graft at the tunnel entry point to overcome the postoperative angulation of intraosseous and intra-articular graft parts

Fatigue Life of Superelastic Springs for an Anterior Cruciate Ligament Prosthesis

1995 ◽  
Vol 05 (C8) ◽  
pp. C8-1223-C8-1228
Author(s):  
N. Hagemeister ◽  
L'H. Yahia ◽  
E. Weynant ◽  
T. Lours
Keyword(s):  
Anterior Cruciate Ligament ◽  
Fatigue Life ◽  
Cruciate Ligament ◽  
Anterior Cruciate ◽  
Ligament Prosthesis

Anterior Cruciate Ligament Rupture Associated with Cardiomyopathy in Three Cats

1991 ◽  
Vol 4 (01) ◽  
pp. 35-37 ◽  
Author(s):  
G. O. Janssens ◽  
D. L. Janssens ◽  
L. A. A. Janssens
Keyword(s):  
Anterior Cruciate Ligament ◽  
Cruciate Ligament ◽  
Anterior Cruciate Ligament Rupture ◽  
Acl Rupture ◽  
Thoracic Radiography ◽  
Ligament Rupture ◽  
Cruciate Ligament Rupture ◽  
Previous Trauma ◽  
History Of ◽  
Anterior Cruciate

SummaryOver a period of 14 years, three cats with anterior cruciate ligament (ACL) rupture were seen in our practice. In all, the rupture had occurred without a history of previous trauma. All were treated surgically. All died within a period of 14 days. The reason of death was in all cases cardiomyopathy. We now suggest that cats with rupture of the anterior cruciate ligament undergo an electrocardiographic recording and eventually an thoracic radiography before surgery is considered. We also suggest that cats with ACL rupture should preferably be treated conservatively.

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