Atomic force microscopy: High resolution dynamic imaging of cellular and molecular structure in health and disease

2013 ◽  
Vol 228 (10) ◽  
pp. 1949-1955 ◽  
Author(s):  
Douglas J. Taatjes ◽  
Anthony S. Quinn ◽  
Jacob H. Rand ◽  
Bhanu P. Jena
Keyword(s):  
Molecular Structure ◽  
Atomic Force Microscopy ◽  
High Resolution ◽  
Dynamic Imaging ◽  
Force Microscopy ◽  
Atomic Force ◽  
Health And Disease

scholarly journals On the early stages of soot formation: Molecular structure elucidation by high-resolution atomic force microscopy

2019 ◽  
Vol 205 ◽  
pp. 154-164 ◽  
Author(s):  
Mario Commodo ◽  
Katharina Kaiser ◽  
Gianluigi De Falco ◽  
Patrizia Minutolo ◽  
Fabian Schulz ◽  
...  
Keyword(s):  
Molecular Structure ◽  
Atomic Force Microscopy ◽  
High Resolution ◽  
Structure Elucidation ◽  
Soot Formation ◽  
Force Microscopy ◽  
Early Stages ◽  
Atomic Force

scholarly journals Automated structure discovery in atomic force microscopy

2020 ◽  
Vol 6 (9) ◽  
pp. eaay6913 ◽  
Author(s):  
Benjamin Alldritt ◽  
Prokop Hapala ◽  
Niko Oinonen ◽  
Fedor Urtev ◽  
Ondrej Krejci ◽  
...  
Keyword(s):  
Molecular Structure ◽  
Atomic Force Microscopy ◽  
Deep Learning ◽  
High Resolution ◽  
Organic Molecules ◽  
Molecular Configuration ◽  
Force Microscopy ◽  
Aromatic Molecules ◽  
Atomic Force ◽  
Functionalized Tips

Atomic force microscopy (AFM) with molecule-functionalized tips has emerged as the primary experimental technique for probing the atomic structure of organic molecules on surfaces. Most experiments have been limited to nearly planar aromatic molecules due to difficulties with interpretation of highly distorted AFM images originating from nonplanar molecules. Here, we develop a deep learning infrastructure that matches a set of AFM images with a unique descriptor characterizing the molecular configuration, allowing us to predict the molecular structure directly. We apply this methodology to resolve several distinct adsorption configurations of 1S-camphor on Cu(111) based on low-temperature AFM measurements. This approach will open the door to applying high-resolution AFM to a large variety of systems, for which routine atomic and chemical structural resolution on the level of individual objects/molecules would be a major breakthrough.

scholarly journals Exploring Intramolecular Methyl–Methyl Coupling on a Metal Surface for Edge-Extended Graphene Nanoribbons

2021 ◽  
Vol 03 (02) ◽  
pp. 128-133
Author(s):  
Zijie Qiu ◽  
Qiang Sun ◽  
Shiyong Wang ◽  
Gabriela Borin Barin ◽  
Bastian Dumslaff ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Atomic Force Microscopy ◽  
High Resolution ◽  
Graphene Nanoribbons ◽  
Scanning Tunneling ◽  
Tunneling Microscopy ◽  
Force Microscopy ◽  
Methyl Methyl ◽  
Atomic Force ◽  
Edge Extension

Intramolecular methyl–methyl coupling on Au (111) is explored as a new on-surface protocol for edge extension in graphene nanoribbons (GNRs). Characterized by high-resolution scanning tunneling microscopy, noncontact atomic force microscopy, and Raman spectroscopy, the methyl–methyl coupling is proven to indeed proceed at the armchair edges of the GNRs, forming six-membered rings with sp3- or sp2-hybridized carbons.

Imaging of Xenopus laevis Oocyte Plasma Membrane in Physiological-Like Conditions by Atomic Force Microscopy

2013 ◽  
Vol 19 (5) ◽  
pp. 1358-1363 ◽  
Author(s):  
Massimo Santacroce ◽  
Federica Daniele ◽  
Andrea Cremona ◽  
Diletta Scaccabarozzi ◽  
Michela Castagna ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Plasma Membrane ◽  
High Resolution ◽  
Membrane Proteins ◽  
Xenopus Laevis ◽  
Functional Study ◽  
Xenopus Laevis Oocyte ◽  
Force Microscopy ◽  
Atomic Force ◽  
Afm Imaging

AbstractXenopus laevis oocytes are an interesting model for the study of many developmental mechanisms because of their dimensions and the ease with which they can be manipulated. In addition, they are widely employed systems for the expression and functional study of heterologous proteins, which can be expressed with high efficiency on their plasma membrane. Here we applied atomic force microscopy (AFM) to the study of the plasma membrane of X. laevis oocytes. In particular, we developed and optimized a new sample preparation protocol, based on the purification of plasma membranes by ultracentrifugation on a sucrose gradient, to perform a high-resolution AFM imaging of X. laevis oocyte plasma membrane in physiological-like conditions. Reproducible AFM topographs allowed visualization and dimensional characterization of membrane patches, whose height corresponds to a single lipid bilayer, as well as the presence of nanometer structures embedded in the plasma membrane and identified as native membrane proteins. The described method appears to be an applicable tool for performing high-resolution AFM imaging of X. laevis oocyte plasma membrane in a physiological-like environment, thus opening promising perspectives for studying in situ cloned membrane proteins of relevant biomedical/pharmacological interest expressed in this biological system.

High-Resolution Frequency-Modulation Atomic Force Microscopy in Liquids Using Electrostatic Excitation Method

2010 ◽  
Vol 3 (6) ◽  
pp. 065205 ◽  
Author(s):  
Ken-ichi Umeda ◽  
Noriaki Oyabu ◽  
Kei Kobayashi ◽  
Yoshiki Hirata ◽  
Kazumi Matsushige ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
High Resolution ◽  
Frequency Modulation ◽  
Force Microscopy ◽  
Atomic Force ◽  
Excitation Method

High-resolution noncontact atomic force microscopy

2009 ◽  
Vol 20 (26) ◽  
pp. 260201-260201 ◽  
Author(s):  
Rubén Pérez ◽  
Ricardo García ◽  
Udo Schwarz
Keyword(s):  
Atomic Force Microscopy ◽  
High Resolution ◽  
Force Microscopy ◽  
Atomic Force
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